Bioinspired porous microspheres for sustained hypoxic exosomes release and vascularized bone regeneration

Gao, Yike; Yuan, Zuoying; Yuan, Xiaojing; Wan, Zhuo; Yu, Yingjie; Zhan, Qiang; Zhao, Yuming; Han, Jianmin; Huang, Jianyong; Xiong, Chunyang; Cai, Qing

doi:10.1016/j.bioactmat.2022.01.041

Cited by 55 publications

(75 citation statements)

References 59 publications

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“…Lastly, the results of the in vitro degradation (Figure 6e-g) showed that the weight loss of PCL porous microspheres began at four weeks when the dissolution of the microsphere surface (marked with red arrows) was also observed under SEM. Compared with PLGA and PLA porous microspheres in other research [4,40], PCL porous microspheres had a slower degradation, but it was much faster than the degradation of solid PCL. The pH of the degradation solution did not change significantly over time, which indicated that PCL porous microspheres would not produce acidic degradation products, different from the porous microspheres made of PLGA and PLA.…”

Section: Characterization Of Pcl Porous Microspheresmentioning

confidence: 74%

Precise Fabrication of Porous Microspheres by Iso-Density Emulsion Combined with Microfluidics

Shi

Zhang

et al. 2022

Polymers

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Polymer porous microspheres with large specific surface areas and good fluidity have promising important applications in the biomedical field. However, controllable fabrication of porous microspheres with precise size, morphology, and pore structure is still a challenge, and phase separation caused by the instability of the emulsion is the main factor affecting the precise preparation of porous microspheres. Herein, a method combining the iso-density emulsion (IDE) template and microfluidics was proposed to realize the controllable preparation of polymer porous microspheres. The IDE exhibited excellent stability with minimal phase separation within 4 h, thus showing potential advantages in the large-scale preparation of porous microspheres. With the IDE template combined microfluidics technique and the use of a customized amphoteric copolymer, PEG-b-polycaprolactone, polycaprolactone (PCL) porous microspheres with porosity higher than 90% were successfully prepared. Afterwards, the main factors, including polymer concentration, water–oil ratio and homogenization time were investigated to regulate the pore structure of microspheres, and microspheres with different pore sizes (1–30 μm) were obtained. PCL porous microspheres exhibited comparable cell viability relative to the control group and good potential as cell microcarriers after surface modification with polydopamine. The modified PCL porous microspheres implanted subcutaneously in rats underwent rapid in vivo degradation and tissue ingrowth. Overall, this study demonstrated an efficient strategy for the precise preparation of porous microspheres and investigated the potential of the as-prepared PCL porous microspheres as cell microcarriers and micro-scaffolds.

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Section: Characterization Of Pcl Porous Microspheresmentioning

confidence: 74%

Precise Fabrication of Porous Microspheres by Iso-Density Emulsion Combined with Microfluidics

Shi

Zhang

et al. 2022

Polymers

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“…The continuously released Tβ4-sEVs (until about 21 days) improved the angiogenesis activity of coronary endothelial cells (CAECs) via the miR-17-5p/PHD3/Hif-1α pathway ( Chen et al, 2022b ). Recently, by electrostatic interaction, Cai et al immobilized hypoxic sEVs (H-sEVs) on PDA-coated injectable porous poly (lactic acid-co-glycolic acid) (PLGA) microspheres, achieving sustained release of H-sEVs until 21 days without decayed bioactivity and promoting vascularized bone regeneration ( Gao et al, 2022 ).…”

Section: Application Avenues Of Msc-sevs On Woundsmentioning

confidence: 99%

Small extracellular vesicles from mesenchymal stem cells: A potential Weapon for chronic non-healing wound treatment

Wei

Liu

et al. 2023

Front. Bioeng. Biotechnol.

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Chronic non-healing wounds have posed a severe threat to patients mentally and physically. Behavior dysregulation of remaining cells at wound sites is recognized as the chief culprit to destroy healing process and hinders wound healing. Therefore, regulating and restoring normal cellular behavior is the core of chronic non-healing wound treatment. In recent years, the therapy with mesenchymal stem cells (MSCs) has become a promising option for chronic wound healing and the efficacy has increasingly been attributed to their exocrine functions. Small extracellular vesicles derived from MSCs (MSC-sEVs) are reported to benefit almost all stages of wound healing by regulating the cellular behavior to participate in the process of inflammatory response, angiogenesis, re-epithelization, and scarless healing. Here, we describe the characteristics of MSC-sEVs and discuss their therapeutic potential in chronic wound treatment. Additionally, we also provide an overview of the application avenues of MSC-sEVs in wound treatment. Finally, we summarize strategies for large-scale production and engineering of MSC-sEVs. This review may possibly provide meaningful guidance for chronic wound treatment with MSC-sEVs.

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“…Bioresorbable porous microspheres made from biodegradable aliphatic polyester (e.g., Poly-l-lactide (PLLA) and Poly-lactic-co-glycolic acid (PLGA)) is considered as a promising biomaterial suitable for minimally invasive drug delivery and tissue regeneration [18] . With surface affinity coatings, these microspheres exhibit desired absorption rate and sustained release of exosomes [19] . Herein, we first investigated the underlying mechanism by figuring out the function of HUVECs treated with GMSCs-Exo in vitro, and explored the role of Wnt/β-catenin signaling pathway for GMSCs-Exo-induced angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…18 With surface-affinity coatings, these microspheres have exhibited a desired absorption rate and sustained release of exosomes. 19 Herein, we rst investigated the underlying mechanism by guring out the function of HUVECs treated with GMSCs-Exo in vitro, and then explored the role of the Wnt/b-catenin signaling pathway for GMSCs-Exo-induced angiogenesis. To locally transplant GMSC-derived exosomes, a porous microsphere matrix was fabricated from PLGA and an amphiphilic block copolymer composed of a hydrophobic PLLA block and hydrophilic poly (ethyl glycol) (PEG) block through a double emulsion method, followed by alkali treatment.…”

Section: Introductionmentioning

confidence: 99%

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Local transplantation of GMSC-derived exosomes to promote vascularized diabetic wound healing by regulating the Wnt/β-catenin pathways

Liu

Yang

et al. 2023

Nanoscale Adv.

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Bioinspired porous microspheres for sustained hypoxic exosomes release and vascularized bone regeneration

Cited by 55 publications

References 59 publications

Precise Fabrication of Porous Microspheres by Iso-Density Emulsion Combined with Microfluidics

Precise Fabrication of Porous Microspheres by Iso-Density Emulsion Combined with Microfluidics

Small extracellular vesicles from mesenchymal stem cells: A potential Weapon for chronic non-healing wound treatment

Local transplantation of GMSC-derived exosomes to promote vascularized diabetic wound healing by regulating the Wnt/β-catenin pathways

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