Bioinspired synthesis of hydroxyapatite nanocrystals in the presence of collagen and <scp>l</scp>‐arginine: Candidates for bone regeneration

Brasinika, D.; Tsigkou, Olga; Tsetsekou, Athena; Missirlis, Yannis F.

doi:10.1002/jbm.b.33413

Cited by 24 publications

(20 citation statements)

References 59 publications

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“…Hydroxyapatite is the major inorganic component of human bone mineral . The tHA synthesized in our study displayed a plate‐like structure, which shows greater similarity to the morphology of natural hydroxyapatite in bone tissue than to rod‐like traditional HA . X‐ray photoelectron spectrometry showed that tHA and traditional HA displayed the same main atomic elements (oxygen, carbon, calcium, and phosphorus), which indicated that they both have the basic chemical characteristic of hydroxyapatite.…”

Section: Discussionmentioning

confidence: 62%

Effect of metformin on human periodontal ligament stem cells cultured with polydopamine‐templated hydroxyapatite

Zhan

Gao

Zhou

et al. 2019

European J Oral Sciences

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Polydopamine‐templated hydroxyapatite (tHA) is a type of nano‐biomaterial that can promote osteogenesis in bone tissue engineering. However, high concentrations of tHA stimulate production of reactive oxygen species (ROS), resulting in cell injury and apoptosis. Metformin has been demonstrated to activate the adenosine monophosphate‐activated protein kinase (AMPK) signaling pathway, which induces autophagy and decreases ROS production to prevent apoptosis. The present study was performed to investigate the potential application of tHA in combination with metformin in periodontal bone tissue engineering. Human periodontal ligament stem cells (hPDLSCs) were exposed to tHA in the presence or absence of metformin, and cytocompatibility and osteogenesis were detected by related assays. Additionally, the autophagy signaling pathway was analyzed by western blotting. Polydopamine‐templated hydroxyapatite, in combination with metformin, substantially reduced ROS production and apoptosis, and enhanced proliferation and osteogenic differentiation of hPDLSCs. Enhanced levels of microtubule‐associated protein 1 light chain 3 II and Beclin‐1 were observed after exposure to tHA plus metformin. Expression of phosphorylated AMPK was increased and that of phosphorylated mammalian target of rapamycin (mTOR) was decreased after exposure to tHA plus metformin. Taken together, our results demonstrate that tHA, combined with metformin, increases the viability of hPDLSCs via the AMPK/mTOR signaling pathway by regulating autophagy and further improving the osteogenic effect.

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Section: Discussionmentioning

confidence: 62%

Effect of metformin on human periodontal ligament stem cells cultured with polydopamine‐templated hydroxyapatite

Zhan

Gao

Zhou

et al. 2019

European J Oral Sciences

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“…In comparison, M2-like macrophages did not significantly increase until a later stage of OTM (between days 21 and 28). As production of M2-phenotype markers such as Arg-1 can promote bone formation, this switch may be important for the cessation of bone resorption and initiation of tissue repair 29 , 30 . However, whether macrophage polarization directly influences OTM is unclear.…”

Section: Discussionmentioning

confidence: 99%

Macrophages mediate corticotomy-accelerated orthodontic tooth movement

Wang

Zhang

Sun

et al. 2018

Sci Rep

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Clinical evidence has suggested that surgical corticotomy of the alveolar bone can accelerate local orthodontic tooth movement (OTM), but the underlying cell and molecular mechanisms remain largely unclear. The present study examined the role of macrophages played in corticotomy-assisted OTM. Orthodontic nickel-titanium springs were applied to the left maxillary first molars of rats or mice to induce OTM with or without corticotomy. Corticotomy enhanced OTM distance by accelerating movement through induction of local osteoclastogenesis and macrophage infiltration during OTM. Further analysis showed that macrophages were polarized toward an M1-like phenotype immediately after corticotomy and then switched to an M2-like phenotype during OTM. The microenvironment of corticotomy induced macrophage infiltration and polarization through the production of TNF-α. More importantly, the amount of OTM induced by corticotomy was significantly decreased after mice were depleted of monocyte/macrophages by injection of liposome-encapsulated clodronate. Further experiments by incubating cultured macrophages with fresh tissue suspension obtained from post-corticotomy gingiva switched the cells to an M1 phenotype through activation of the nuclear factor-κB (NF-κB) signaling pathway, and to an M2 phenotype through activation of the JAK/STAT3 signaling pathway. Our results suggest that corticotomy induces macrophage polarization first by activating the NF-κB signaling pathway and later by activating the JAK/STAT3 signaling pathway, and that these processes contribute to OTM by triggering production of inflammatory cytokines and osteoclastogenesis.

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“…It was proved that the best results in the integration with the bone tissue just demonstrated those materials that are comprised of the analogue of the mineral compound in the bone human tissue -calcium hydroxyapatite (HAP) and organic matrix used for the improvement of the reparative processes, increase in the resorption rate and strength [1,3,[7][8][9]. Complex materialsbiocomposites, elaborated on the basis of calcium hydroxyapatite, collagen, chitosan or any other biodegradable organic matrix have a lot of advantages for dental applications implying the control of their bioactivity and resorption rate [2,8,10].…”

Section: Introductionmentioning

confidence: 99%

“…The generally accepted trend for reducing the risks of rejection of the xenogenic samples involves the incorporation of the medical preparations or some organic agents [7,9,13]. However, the rate of biodegradation and osseointegration of synthetic samples can be determined based on their surface characteristics as well as performed preliminary treatment including the side action effect of introduced agents.…”

Section: Introductionmentioning

confidence: 99%

Comparative characteristics of the xenogenic synthetic biomimetic composite and spongy bone tissue for the goals of osteoplasty in dentistry

Seredin¹,

Goloshchapov²,

Gushchin³

et al. 2019

Russ Open Med J

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Background -The structural and morphological features in the natural and synthetic biomimetic composites should be defined and compared to determine the optimal artificial material for osteoplastic tasks. Methods -The sample of osteoplastic material of the xenogenic group representing the treated bone blocks of cattle, sample of a spongy bone tissue of a human lower jaw and a sample of biomimetic carbonate-substituted calcium hydroxyapatite synthesized with the use of birds' egg-shells were studied using X-ray diffraction (XRD) and Fourier Transmission Infrared spectroscopy (FTIR) for the structure, phase and molecular composition analysis, X-ray spectral microanalysis (XMA) was applied for chemical element composition, optical and scanning electron microscopy (SEM) were used for a surface features analysis of the materials with a porous structure.Results -The analysis of the xenogenic material as well as the bone tissue sample by XRD and FTIR reveal that the samples are comprised of a single crystalline phase, i.e. a carbonate-substituted calcium hydroxyapatite (CHAP) with distortions in the crystalline structure. The calculations of the hydroxyapatite crystallites size in the samples of the bone tissue and the xenogenic materials by the XRD data are 35.2±1.5 nm and 11.1±0.6 nm respectively. A different content of the amide and carboxyl groups in the bone tissue and in the osteoplastic material were found using IR-spectroscopy in the 1,800-1,200 cm -1 indicates the presence of protein structures in the latter which are different in the molecular structure from those presented in the native human bone tissue. The redistribution of the vibration modes in the spectral ranges 3000-2800 and 800-500 cm -1 also confirm this suggestion. Scanning electron microscopy demonstrated that xenogenic materials possess the morphology and a system of conjugated pores similar to those of the human bone tissue. At the micrometer level the pores that are present in the xenograft coincide in their size with those in the native bone matrix (3-6 m). Optical microscopy investigations shows that in the osteoplastic xenogenic materials at the macrolevel there are no pores with the size of 0.25-1.5 mm, which are observed in the samples of the native bone tissue. Conclusion -Based on the results of comparison of the structural, molecular and morphological properties of the studied samples, it is possible to conclude that the samples of the osteoplastic materials do not completely correspond to the spongy human bone tissue, which imposes certain constraints on their practical applications. Comparison of the structure, phase and molecular composition as well as the morphology of the samples of native porous bone tissue and xenogenic materials allowed us to identify the principal differences between the two materials as well as to design the requirements and guidelines for manufacturers on extra processing of a xenogenic material for eliminating the factors affecting the quality of implantation and resorption of a biomimetic composite: macrop...

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Bioinspired synthesis of hydroxyapatite nanocrystals in the presence of collagen and l‐arginine: Candidates for bone regeneration

Cited by 24 publications

References 59 publications

Effect of metformin on human periodontal ligament stem cells cultured with polydopamine‐templated hydroxyapatite

Effect of metformin on human periodontal ligament stem cells cultured with polydopamine‐templated hydroxyapatite

Macrophages mediate corticotomy-accelerated orthodontic tooth movement

Comparative characteristics of the xenogenic synthetic biomimetic composite and spongy bone tissue for the goals of osteoplasty in dentistry

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