Bioinspirierte Klebstoffe zur Anwendung in wässrigen Flüssigkeiten

Scheibel, Thomas; Trossmann, Vanessa T.; Lechner, Annika; Bargel, Hendrik; Humeník, Martin; Z̆urovec, Michal

doi:10.1007/s35145-022-0554-6

Cited by 1 publication

(1 citation statement)

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“…The building blocks of these materials are arranged in a bottom‐up manner into complex nano‐ to macroscale hierarchical architectures, which allow for preparation of sustainable, multifunctional materials with unique combinations of strength and toughness (Lendel & Solin, 2021 ; Ling et al, 2018 ; Lombardo et al, 2020 ; Olson et al, 2021 ; Percebom et al, 2018 ; Shen et al, 2021 ; Wegst et al, 2015 ). There is a growing focus on proteinaceous nanofibrils made of recombinant spider silk proteins or silk fibroin from Bombyx mori , which can be manufactured into gels and intensively applied in 3D printing and tissue engineering techniques (de Oliveira et al, 2023 ; Humenik et al, 2014 ; Kamada et al, 2023 ; Kim et al, 2021 ; Lamberger et al, 2022 ; Schacht et al, 2015 ; Scheibel et al, 2022 ; Steiner et al, 2021 ; Sun & Marelli, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Only kosmotrope anions trigger fibrillization of the recombinant core spidroin eADF4(C16) from Araneus diadematus

Hovanová,

Hovan,

Humenik

et al. 2023

Protein Science

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Recombinant core spidroin eADF4(C16) has received increasing attention due to its ability to form micro‐ and nano‐structured scaffolds, which are based on nanofibrils with great potential for biomedical and biotechnological applications. Phosphate anions have been demonstrated to trigger the eADF4(C16) self‐assembly into cross‐beta fibrils. In the present work, we systematically addressed the effect of nine sodium anions, namely SO42−, HPO42− (Pi), F−, Cl−, Br−, NO3−, I−, SCN−, and ClO4− from the Hofmeister series on the in vitro self‐assembly kinetics of eADF4(C16). We show that besides the phosphate anions, only kosmotropic anions such as sulfate and fluoride can initiate the eADF4(C16) fibril formation. Global analysis of the self‐assembly kinetics, utilizing the platform AmyloFit, showed the nucleation‐based mechanism with a major role of a secondary nucleation, surprisingly independent of the type of the kosmotropic anion. The rate constant of the fibril elongation in mixtures of phosphate anions with other studied anions correlated with their kosmotropic or chaotropic position in the Hofmeister series. Our findings suggest an important role of anion hydration in the eADF4(C16) fibrillization process.This article is protected by copyright. All rights reserved.

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