Biolistic and other non-Agrobacterium technologies of plant transformation

Matsumoto, Tracie K.

doi:10.1016/b978-0-12-381466-1.00008-0

Cited by 16 publications

(18 citation statements)

References 67 publications

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“…One of the advantages of the biolistic method over AMT is related to the variety of species transformed by the former (Matsumoto and Gonsalves, 2012;Cunningham et al, 2018). In general, this method is preferred for rapid assays using transient expressions, such as protein localization, the functional analysis of promoters, and transcription factor characterization (Lenka et al, 2015(Lenka et al, , 2018Wang et al, 2018).…”

Section: Conventional Plant Biomolecule Delivery Approaches and Their Limitationsmentioning

confidence: 99%

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

et al. 2021

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Agriculture is an important source of human food. However, current agricultural practices need modernizing and strengthening to fulfill the increasing food requirements of the growing worldwide population. Genome editing (GE) technology has been used to produce plants with improved yields and nutritional value as well as with higher resilience to herbicides, insects, and diseases. Several GE tools have been developed recently, including clustered regularly interspaced short palindromic repeats (CRISPR) with nucleases, a customizable and successful method. The main steps of the GE process involve introducing transgenes or CRISPR into plants via specific gene delivery systems. However, GE tools have certain limitations, including time-consuming and complicated protocols, potential tissue damage, DNA incorporation in the host genome, and low transformation efficiency. To overcome these issues, nanotechnology has emerged as a groundbreaking and modern technique. Nanoparticle-mediated gene delivery is superior to conventional biomolecular approaches because it enhances the transformation efficiency for both temporal (transient) and permanent (stable) genetic modifications in various plant species. However, with the discoveries of various advanced technologies, certain challenges in developing a short-term breeding strategy in plants remain. Thus, in this review, nanobased delivery systems and plant genetic engineering challenges are discussed in detail. Moreover, we have suggested an effective method to hasten crop improvement programs by combining current technologies, such as speed breeding and CRISPR/Cas, with nanotechnology. The overall aim of this review is to provide a detailed overview of nanotechnology-based CRISPR techniques for plant transformation and suggest applications for possible crop enhancement.

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Section: Conventional Plant Biomolecule Delivery Approaches and Their Limitationsmentioning

confidence: 99%

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

et al. 2021

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“…The biolistic method is not something entirely new in the world of genetic engineering. It was first identified and introduced to the field in the late 1980s, where it was initially tested on plants for transformation studies [ 23 , 38 ]. The word “biolistic” originated from the term biology, and ballistics refers to its mode of action that is similar to a gun [ 9 , 23 , 38 ].…”

Section: Main Textmentioning

confidence: 99%

“…It was first identified and introduced to the field in the late 1980s, where it was initially tested on plants for transformation studies [ 23 , 38 ]. The word “biolistic” originated from the term biology, and ballistics refers to its mode of action that is similar to a gun [ 9 , 23 , 38 ]. The biolistic approach is one of the commonly used techniques in plant transformation, and other methods will only be considered when the biolistic approach is not suitable for the transformation process [ 38 ].…”

Section: Main Textmentioning

confidence: 99%

“…The word “biolistic” originated from the term biology, and ballistics refers to its mode of action that is similar to a gun [ 9 , 23 , 38 ]. The biolistic approach is one of the commonly used techniques in plant transformation, and other methods will only be considered when the biolistic approach is not suitable for the transformation process [ 38 ]. The whole concept of biolistic is depending on high pressure, whereby the desired DNA construct will be projected into the host using a gene gun at high speed with the help of pressurized gas [ 6 , 9 , 23 , 38 , 63 ].…”

Section: Main Textmentioning

confidence: 99%

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Gene introduction approaches in chloroplast transformation and its applications

Kumar

Ling

2021

Journal of Genetic Engineering and Biotechnology

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Background Chloroplast is a type of plastid that is believed to be originated from ancestral cyanobacteria. Chloroplast besides being a major component for photosynthesis, also takes part in another major plant metabolism, making it one of the major components of plants. Main body Chloroplast transformation is an alternative and better genetic engineering approach compared to the nuclear transformation that has been widely applied in plant genetic engineering. Chloroplast transformation has exhibited various positive effects as compared to nuclear transformation. This is a more preferred technique by researchers. To carry out chloroplast transformation, the vector design must be performed, and a selectable marker needs to be incorporated before the chloroplast could uptake the construct. The common way of introducing a gene into the host, which is the chloroplast, involves the biolistic, PEG-mediated, carbon nanotubes carriers, UV-laser microbeam, and Agrobacterium-mediated transformation approaches. Apart from discussing the processes involved in introducing the gene into the chloroplast, this review also focuses on the various applications brought about by chloroplast transformation, particularly in the field of agriculture and environmental science. Conclusion Chloroplast transformation has shown a lot of advantages and proven to be a better alternative compared to nuclear genome transformation. Further studies must be conducted to uncover new knowledge regarding chloroplast transformation as well as to discover its additional applications in the fields of biotechnology.

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“…1987'de Cornell Üniversitesi'nde tahıllarda gen aktarımının üstesinden gelebilmek için geliştirilmiş (Sanford et al, 1987) olsa da hemen hemen tüm bitkilerin tüm doku tipleri için uygundur (Hansen and Wright, 1999). Agrobacterium aracılığıyla transformasyon dışında en çok kullanılan transformasyon yöntemidir (Matsumoto and Gonsalves, 2012). Doğrudan transformasyon yöntemleri içinde oldukça popüler olmasının sebebi; biyolistik yöntemin birçok tür, organel, bakteri, fungus ve hatta hayvansal hücrelerde kullanılabilmesi (Rakoczy-Trojanowska, 2002); sürecin kısa sürede tamamlanması, transgenik bitki üretiminin düşük maliyet gerektirmesi; çoklu genlerin veya kimerik DNA'nın (farklı organizmalardan ve homolog olmayan kaynaklardan gelen DNA'ların birleşmesi) aktarım kolaylığıdır (Rivera et al, 2012).…”

Section: Biyolistik (Gen Tabancası)unclassified

Gene Technology and Plant Genetic Transformation Methods

Macar¹,

Macar²,

Yalçın³

et al. 2017

AKU-J. Sci. Eng.

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Genetik mühendisliği uygulamaları tarla bitkilerinin modern moleküler ıslahında önemli biyoteknolojik araçlar haline gelmiştir. 1990lardan itibaren, bitki hücrelerine yabancı DNA'yı aktarmak için çeşitli yeni ve etkili genetik transformasyon yöntemleri geliştirilmiştir. Yaygın gen aktarma metodları genellikle doğrudan ve dolaylı transformasyon diye ayrılmaktadır. Her tekniğin belli avantajları ve dezavantajları olsa da, günümüzde transgenik bitkilerin üretimi birçok bitki türü için rutin bir süreç haline gelmiştir. Yine de, tekrarlanabilir bir transformasyon metodu elde edebilmek için ihmal edilmemesi gereken birçok önemli etken vardır. Bu çalışma transgen teknolojisini kısaca gözden geçirmeyi ve bitki genetik transformasyonu için günümüzde kullanılmakta olan metodları yeniden derlemeyi amaçlamaktadır.

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Biolistic and other non-Agrobacterium technologies of plant transformation

Cited by 16 publications

References 67 publications

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

Gene introduction approaches in chloroplast transformation and its applications

Gene Technology and Plant Genetic Transformation Methods

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