Biolistic introduction of a synthetic Bt gene into elite maize

Hill, Martha; Launis, Karen; Bowman, Cindy; McPherson, K.; Dawson, John F.; Watkins, J.; Koziel, Michael G.; Wright, Martha

doi:10.1007/978-94-011-0357-2_15

Cited by 8 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, the results of a 1-year feeding trial with a GM maize MON810 variety, its near-isogenic non-GM comparator and an additional conventional maize variety are presented. The transgenic trait MON810 consists of a Bacillus thuringiensis ( Bt )-derived gene, namely a truncated cry1Ab gene encoding an insecticidal protein (δ-endotoxin) (Schnepf et al 1998 ), for the control of some lepidopteran insect pests such as the European corn borer ( Ostrinia nubilalis ) (Hill et al 1995 ). The selected MON810 variety was one of the most widely used by farmers in Catalonia, Spain.…”

Section: Introductionmentioning

confidence: 99%

One-year oral toxicity study on a genetically modified maize MON810 variety in Wistar Han RCC rats (EU 7th Framework Programme project GRACE)

et al. 2016

View full text Add to dashboard Cite

The GRACE (GMO Risk Assessment and Communication of Evidence; www.grace-fp7.eu) project was funded by the European Commission within the 7th Framework Programme. A key objective of GRACE was to conduct 90-day animal feeding trials, animal studies with an extended time frame as well as analytical, in vitro and in silico studies on genetically modified (GM) maize in order to comparatively evaluate their use in GM plant risk assessment. In the present study, the results of a 1-year feeding trial with a GM maize MON810 variety, its near-isogenic non-GM comparator and an additional conventional maize variety are presented. The feeding trials were performed by taking into account the guidance for such studies published by the EFSA Scientific Committee in 2011 and the OECD Test Guideline 452. The results obtained show that the MON810 maize at a level of up to 33 % in the diet did not induce adverse effects in male and female Wistar Han RCC rats after a chronic exposure.Electronic supplementary materialThe online version of this article (doi:10.1007/s00204-016-1798-4) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

One-year oral toxicity study on a genetically modified maize MON810 variety in Wistar Han RCC rats (EU 7th Framework Programme project GRACE)

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In the present study, the results of two 90-day feeding trials with two different GM maize MON810 varieties performed by taking into account the guidance for such studies published by the EFSA Scientific Committee in 2011 and the OECD TG 408 are presented. The transgenic trait MON810 consists of a Bacillus thuringiensis ( Bt )-derived gene, namely a truncated cry1Ab gene encoding an insecticidal protein (δ-endotoxin; Schnepf et al 1998 ), for the control of some lepidopteran insect pests such as the European corn borer ( Ostrinia nubilalis ; Hill et al 1995 ). The two selected varieties were the two most widely used by farmers in Catalonia, Spain, and were chosen to explore whether the genetic background had an influence on the outcome of the feeding trials.…”

Section: Introductionmentioning

confidence: 99%

Ninety-day oral toxicity studies on two genetically modified maize MON810 varieties in Wistar Han RCC rats (EU 7th Framework Programme project GRACE)

et al. 2014

View full text Add to dashboard Cite

The GMO Risk Assessment and Communication of Evidence (GRACE; www.grace-fp7.eu) project is funded by the European Commission within the 7th Framework Programme. A key objective of GRACE is to conduct 90-day animal feeding trials, animal studies with an extended time frame as well as analytical, in vitro and in silico studies on genetically modified (GM) maize in order to comparatively evaluate their use in GM plant risk assessment. In the present study, the results of two 90-day feeding trials with two different GM maize MON810 varieties, their near-isogenic non-GM varieties and four additional conventional maize varieties are presented. The feeding trials were performed by taking into account the guidance for such studies published by the EFSA Scientific Committee in 2011 and the OECD Test Guideline 408. The results obtained show that the MON810 maize at a level of up to 33 % in the diet did not induce adverse effects in male and female Wistar Han RCC rats after subchronic exposure, independently of the two different genetic backgrounds of the event.Electronic supplementary materialThe online version of this article (doi:10.1007/s00204-014-1374-8) contains supplementary material, which is available to authorized users.

show abstract

“…This protein is produced in all plant tissues as a result of introduction of the bacterial cry1Ab transgene into the maize genome [6]. While the bacterial Cry1Ab protein has been extensively used as an organic insecticide [7], its expression in transgenic maize could potentially alter its structure which may render it allergenic or otherwise harmful upon ingestion [8].…”

Section: Introductionmentioning

confidence: 99%

Effects of Feeding Bt Maize to Sows during Gestation and Lactation on Maternal and Offspring Immunity and Fate of Transgenic Material

et al. 2012

View full text Add to dashboard Cite

BackgroundWe aimed to determine the effect of feeding transgenic maize to sows during gestation and lactation on maternal and offspring immunity and to assess the fate of transgenic material.Methodology/Principal FindingsOn the day of insemination, sows were assigned to one of two treatments (n = 12/treatment); 1) non-Bt control maize diet or 2) Bt-MON810 maize diet, which were fed for ∼143 days throughout gestation and lactation. Immune function was assessed by leukocyte phenotyping, haematology and Cry1Ab-specific antibody presence in blood on days 0, 28 and 110 of gestation and at the end of lactation. Peripheral-blood mononuclear cell cytokine production was investigated on days 28 and 110 of gestation. Haematological analysis was performed on offspring at birth (n = 12/treatment). Presence of the cry1Ab transgene was assessed in sows' blood and faeces on day 110 of gestation and in blood and tissues of offspring at birth. Cry1Ab protein presence was assessed in sows' blood during gestation and lactation and in tissues of offspring at birth. Blood monocyte count and percentage were higher (P<0.05), while granulocyte percentage was lower (P<0.05) in Bt maize-fed sows on day 110 of gestation. Leukocyte count and granulocyte count and percentage were lower (P<0.05), while lymphocyte percentage was higher (P<0.05) in offspring of Bt maize-fed sows. Bt maize-fed sows had a lower percentage of monocytes on day 28 of lactation and of CD4+CD8+ lymphocytes on day 110 of gestation, day 28 of lactation and overall (P<0.05). Cytokine production was similar between treatments. Transgenic material or Cry1Ab-specific antibodies were not detected in sows or offspring.Conclusions/SignificanceTreatment differences observed following feeding of Bt maize to sows did not indicate inflammation or allergy and are unlikely to be of major importance. These results provide additional data for Bt maize safety assessment.

show abstract

Biolistic introduction of a synthetic Bt gene into elite maize

Cited by 8 publications

References 13 publications

One-year oral toxicity study on a genetically modified maize MON810 variety in Wistar Han RCC rats (EU 7th Framework Programme project GRACE)

One-year oral toxicity study on a genetically modified maize MON810 variety in Wistar Han RCC rats (EU 7th Framework Programme project GRACE)

Ninety-day oral toxicity studies on two genetically modified maize MON810 varieties in Wistar Han RCC rats (EU 7th Framework Programme project GRACE)

Effects of Feeding Bt Maize to Sows during Gestation and Lactation on Maternal and Offspring Immunity and Fate of Transgenic Material

Contact Info

Product

Resources

About