Biolistic Transformation of Japonica Rice Varieties

Banakar, Raviraj; Wang, Kan

doi:10.1007/978-1-0716-0356-7_8

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…Although several delivery systems have been used, including those based on biologicals [e.g., agrobacteria (30)], biolistic particles (31)(32)(33) [e.g., tungsten particles (34)], and nanomaterials (e.g., singlewall carbon nanotubes) (35)(36)(37)(38)(39)(40)(41)(42), some challenges, such as low transformation or delivery efficiency and potential damage or perturbation of the plant (43, 44), remain. For example, a broccoli riboswitch RNA aptamer was introduced into plant cells with the help of Agrobacterium tumefaciens GV3101 and was used to detect mRNA transcripts for transgene identification and track an RNA virus invasion in plant cells (45).…”

Section: Introductionmentioning

confidence: 99%

Efficient delivery of a DNA aptamer-based biosensor into plant cells for glucose sensing through thiol-mediated uptake

Mou

Xue

et al. 2022

Sci. Adv.

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DNA aptamers have been widely used as biosensors for detecting a variety of targets. Despite decades of success, they have not been applied to monitor any targets in plants, even though plants are a major platform for providing oxygen, food, and sustainable products ranging from energy fuels to chemicals, and high-value products such as pharmaceuticals. A major barrier to progress is a lack of efficient methods to deliver DNA into plant cells. We herein report a thiol-mediated uptake method that more efficiently delivers DNA into Arabidopsis and tobacco leaf cells than another state-of-the-art method, DNA nanostructures. Such a method allowed efficient delivery of a glucose DNA aptamer sensor into Arabidopsis for sensing glucose. This demonstration opens a new avenue to apply DNA aptamer sensors for functional studies of various targets, including metabolites, plant hormones, metal ions, and proteins in plants for a better understanding of the biodistribution and regulation of these species and their functions.

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Section: Introductionmentioning

confidence: 99%

Efficient delivery of a DNA aptamer-based biosensor into plant cells for glucose sensing through thiol-mediated uptake

Mou

Xue

et al. 2022

Sci. Adv.

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“…Compared to bulking up one single callus line, the collection of multiple callus line materials would allow us to conduct bombardment experiments on newly established callus lines sooner, within a few weeks of the callus initiation. This is similar to a protocol of biolistic-mediated transformation of rice ( Banakar and Wang, 2020 ). Most importantly, this practice helps to avoid the uncertainty of using one particular callus line in obtaining transgenic events.…”

Section: Discussionmentioning

confidence: 99%

Transformation of Teosinte (Zea mays ssp. parviglumis) via Biolistic Bombardment of Seedling-Derived Callus Tissues

et al. 2021

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Modern maize exhibits a significantly different phenotype than its wild progenitor teosinte despite many genetic similarities. Of the many subspecies of Zea mays identified as teosinte, Zea mays ssp. parviglumis is the most closely related to domesticated maize. Understanding teosinte genes and their regulations can provide great insights into the maize domestication process and facilitate breeding for future crop improvement. However, a protocol of genetic transformation, which is essential for gene functional analyses, is not available in teosinte. In this study, we report the establishment of a robust callus induction and regeneration protocol using whorl segments of seedlings germinated from mature seeds of Zea parviglumis. We also report, for the first time, the production of fertile, transgenic teosinte plants using the particle bombardment. Using herbicide resistance genes such as mutant acetolactate synthase (Als) or bialaphos resistance (bar) as selectable markers, we achieved an average transformation frequency of 4.17% (percentage of independent transgenic events in total bombarded explants that produced callus). Expression of visual marker genes of red fluorescent protein tdTomato and β-glucuronidase (gus) could be detected in bombarded callus culture and in T1 and T2 progeny plants. The protocol established in this work provides a major enabling technology for research toward the understanding of this important plant in crop domestication.

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“…The rice cultivar Kitaake was used for biolistic-mediated transformation based on the protocol described previously ( Frame et al 2000 ; Banakar and Wang 2020 ). Briefly, Kitaake callus cells were induced on callus induction medium (Murashige and Skoog [MS] salts) (Cat.…”

Section: Methodsmentioning

confidence: 99%

Efficient protein tagging and cis-regulatory element engineering via precise and directional oligonucleotide-based targeted insertion in plants

et al. 2023

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Efficient and precise targeted insertion holds great promise but remains challenging in plant genome editing. An efficient non-homologous end-joining-mediated targeted insertion method was recently developed by combining CRISPR/SpCas9 gene editing with phosphorothioate modified double-stranded oligodeoxynucleotides (dsODNs). Yet this approach often leads to imprecise insertions with no control over the insertion direction. Here, we compared the influence of chemical protection of dsODNs on efficiency of targeted insertion. We observed that CRISPR/SpCas9 frequently induced staggered cleavages with 1-nucleotide 5´overhangs; we also evaluated the effect of donor end structures on the direction and precision of targeted insertions. We demonstrate that chemically protected dsODNs with 1-nucleotide 5´ overhangs significantly improved the precision and direction control of target insertions in all tested CRISPR targeted sites. We applied this method to endogenous gene tagging in green foxtail (Setaria viridis), and engineering of cis-regulatory elements for disease resistance in rice (Oryza sativa). We directionally inserted two distinct transcription activator-like effector binding elements into the promoter region of a recessive rice bacterial blight resistance gene with up to 24.4% efficiency. The resulting rice lines harboring heritable insertions exhibited strong resistance to infection by the pathogen Xanthomonas oryzae pv. oryzae in an inducible and strain-specific manner.

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Biolistic Transformation of Japonica Rice Varieties

Cited by 7 publications

References 18 publications

Efficient delivery of a DNA aptamer-based biosensor into plant cells for glucose sensing through thiol-mediated uptake

Efficient delivery of a DNA aptamer-based biosensor into plant cells for glucose sensing through thiol-mediated uptake

Transformation of Teosinte (Zea mays ssp. parviglumis) via Biolistic Bombardment of Seedling-Derived Callus Tissues

Efficient protein tagging and cis-regulatory element engineering via precise and directional oligonucleotide-based targeted insertion in plants

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