The search for more efficient alternatives to remediation of effluents, both economically and environmentally, are starting points for several studies of new materials for this purpose. Thus, biochar (BC), which has a large number of functional groups and high porosity, and zero valence iron (Fe 0 ), which has a great capacity to reduce other elements, are good alternatives for the remediation of liquid effluents, because advantageous physicochemical characteristics that are also environmentally safer without environmental toxicity. Thus, the objective of this study is to verify the efficiency of eucalyptus bark (BCEuc) and sugarcane bagasse (BCCana) biochars and Fe 0 in the retention of elements and neutralization of the pH of an acid mine drainage (DAM) of the mine Osamu Utsumi, of Nuclear Industries of Brazil located in the municipality of Caldas (MG). For this, a column experiment was performed, where DAM was pumped and the exit solutions were collected and chemically and physicochemically analyzed by ICP-OES (Al, As, Ca, K, Mg, Mn, Na, Zn), ICP-MS (ETR, Th and U), pHmeter and sensors of electrical conductivity and Eh. Column reactive materials (BCEuc, BCCana and Fe 0 ) were analyzed by FTIR, DRX and MEV. With the analysis of the results, both the BC´s and Fe 0 presented high rates of removal of U and As of the initial solution being highly efficient (up to 75.6% for U and 25% for As). These results indicates that these materials can be used for applications in the form of a permeable reactive barrier. It was also verified by the presence of crystals with rare earth elements (La, Ce and Nd) in the MEV analyzes that the bio-carbon can be used for the recovery of these elements of DAM