Biologic characteristics of esophageal epithelial dysplasia assessed by proliferating cell nuclear antigen

Abstract

PCNA expression may be a useful marker to reflect the biologic characteristics of esophageal dysplasia, suggesting that a high grade of dysplasia is as serious a lesion as esophageal carcinoma.

Expression of cell cycle regulatory proteins in the multistep process of oesophageal carcinogenesis: stepwise over‐expression of cyclin E and p53, reduction of p21WAF1/CIP1 and dysregulation of cyclin D1 and p27KIP1

“…For example, a long‐term follow‐up study of patients with oesophageal dysplasia showed that 30% of the cases progressed to squamous cell carcinoma over a follow‐up period of 2.5– 6.5 years 3 . Possible molecular bases of this process have been examined and several abnormalities have been described in dysplasia, including genetic instability, DNA aneuploidy, loss of heterozygosity, mutation of the tumour suppressor gene p53 , and a high proliferating cell nuclear antigen (PCNA) index 4–10 . However, much remains to be clarified about the dysplasia–carcinoma sequence of the oesophagus.…”

In situ hybridization for the detection of telomerase RNA in the progression from barrett's esophagus to esophageal adenocarcinoma

“…Autoradiographic studies utilizing tritiated thymidine reported an increase in the population of proliferative cells in some patients with Barrett's metaplasia. 14 In addition, immunohistochemical studies using antibodies against proliferation-associated antigens, such as proliferating cell nuclear antigen (PCNA) [15][16][17][18] and Ki-67, 19,20 reported a positive correlation between cell proliferation and increasing degrees of dysplasia in Barrett's epithelium. Other studies utilizing flow cytometric analysis to assess Ki-67 labeling, however, found no differences in Ki-67 expression among Barrett's without dysplasia, low grade dysplasia, high grade dysplasia, or adenocarcinoma.…”

Histological and biological characteristics of esophageal dysplasia

“…In this study, we estimated the cell proliferative activity and carcinogenesis of esophageal dysplastic lesions using PCNA analysis [10]. PCNA was first reported as one of the nuclear antigens reacting with an autoantibody in the sera of patients with systemic lupus erythematosus.…”

“…The PCNA LI is expressed as a percentage of the positive cells [10]. In normal epithelium, the PCNA LI was low (7.5% ± 4.1%) in the basal layer, and about 17.3% ± 7.3% in the parabasal layer (Table 3).…”

“…Multiplicity of squamous cell carcinoma and intraepithelial spread of main lesions were also frequently observed [7,8]. We performed a cytophotometric DNA analysis on chemically induced esophageal carcinoma in rats [9] and used immunohistochemical detection of proliferating cell nuclear antigen (PCNA) for resected human esophageal specimens [10]. Results indicated that the biological nature of the dysplasia was similar to a cancer lesion.…”