Biologic Properties of Cell Lines Derived From Canine Mammary Carcinomas23

Abstract

Six canine mammary tumor cell lines (5 epithelial, 1 myoepithelial) were established from dogs with spontaneous mammary carcinomas. The cells were characterized for features of morphology and growth patterns on plastic, ultrastructure, cloning efficiency in soft agar, tumorigenicity in athymic nude mice, and hormonal receptor status. Biologic properties of the cell lines were distinct and preserved during long-term culture in vitro. All cell lines were clonogenic, 5 of 6 were tumorigenic, and 3 of 5 were receptor positive for estrogen and/or progesterone.

Alterations in CDK1 expression and nuclear/nucleolar localization following induction in a spontaneous canine mammary cancer model

“…Because regulation of cell cycle progression is so well conserved and controlled, we hypothesized that the most likely explanation for such different responses may be found in the characteristics of different cell/tumor types or the specific mutations responsible for transformation/immortality in different cell lines. To test this hypothesis, six CMT-derived cell lines with well-characterized biological properties [Wolfe et al, 1986] were evaluated for CDK1 expression and phase of cell cycle arrest in response to okadaic acid treatment. Five of the CMT cell lines were derived from spontaneous epithelial cell tumors and a sixth (CMT47) was derived from a spontaneous myoepithelial cell tumor [Wolfe et al, 1986].…”

“…To test this hypothesis, six CMT-derived cell lines with well-characterized biological properties [Wolfe et al, 1986] were evaluated for CDK1 expression and phase of cell cycle arrest in response to okadaic acid treatment. Five of the CMT cell lines were derived from spontaneous epithelial cell tumors and a sixth (CMT47) was derived from a spontaneous myoepithelial cell tumor [Wolfe et al, 1986]. Although these cell lines are all immortal and transformed, they were all independently derived from different animals and thus likely represent a range of different transforming mutations.…”

“…CMTderived cell lines (CMT9, CMT12, CMT25, CMT27, CMT28, CMT47) were obtained from Dr. L.G. Wolfe and cultured as described [Wolfe et al, 1986]. Cells were treated with 19 nM okadaic acid (Moana Bioproducts, Hawaii), from a 2.0 mM stock solution, in growth medium for 12-48 h.…”

“…Mammary cancers in both species develop spontaneously and are due to somatic mutations [MacEwen et al, 1982;Wolfe et al, 1986;MacEwen, 1990]. Mammary carcinomas are the most common malignancy of female dogs although the incidence of mammary tumors is three times higher than in women [Fidler and Brody, 1967;MacEwen et al, 1982;Wolfe et al, 1986;MacEwen, 1990].…”

“…Mammary cancers in both species develop spontaneously and are due to somatic mutations [MacEwen et al, 1982;Wolfe et al, 1986;MacEwen, 1990]. Mammary carcinomas are the most common malignancy of female dogs although the incidence of mammary tumors is three times higher than in women [Fidler and Brody, 1967;MacEwen et al, 1982;Wolfe et al, 1986;MacEwen, 1990]. Other similarities between mammary cancers of women and dogs include adenocarcinoma as the malignant cell type, metastasis and rapid evolution, hormonal dependency and presence of hormonal receptors, and an age-dependent occurrence [Weije et al, 1972;MacEwen, 1990;MacEwen andWithrow, 1996, 2001].…”