Biologic variability of sperm DNA denaturation in infertile men

Zini, Armand; Kamal, Khaled; Phang, Donna; Willis, John; Jarvi, Keith

doi:10.1016/s0090-4295(01)01180-3

Cited by 120 publications

(91 citation statements)

References 13 publications

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“…That study, in which DNA fragmentation was assessed by TUNEL assay in swim-up selected spermatozoa of 35 samples, found a correlation between DNA fragmentation and vitality detected by HOST as well as sperm motility and morphology. DNA fragmentation has also reported to correlate to sperm concentration, motility and morphology [56], sperm concentration and motility [63], morphology only [10] and concentration only [31,52]. However, others have reported no correlation between DNA integrity and standard semen parameters [23,24,38,39].…”

Section: Discussionmentioning

confidence: 99%

Sperm Dna Integrity: Correlation With Sperm Plasma Membrane Integrity In Semen Evaluated For Male Infertility

Moskovtsev

Willis

Azad

et al. 2005

Archives of Andrology

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Section: Discussionmentioning

confidence: 99%

Sperm Dna Integrity: Correlation With Sperm Plasma Membrane Integrity In Semen Evaluated For Male Infertility

Moskovtsev

Willis

Azad

et al. 2005

Archives of Andrology

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“…15,22,29,53,[62][63][64][65][66][67][68][69] In addition, sperm DNA damage is a more reliable outcome measure than sperm concentration or motility because measures of sperm DNA damage exhibit a lower degree of biological variability than conventional semen parameters. [70][71][72] Treatment with oral antioxidants has generally been associated with improvement in sperm DNA integrity and in some cases pregnancy rates after assisted reproduction, although most of these studies are small and few are randomized placebo-controlled trials ( Table 3).…”

Section: Relationship Between Oxidative Stress and Sperm Dysfunctionmentioning

confidence: 99%

Antioxidant therapy in male infertility: fact or fiction?

Zini¹,

Alhathal²

2011

Asian J Androl

Self Cite

110

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Infertile men have higher levels of semen reactive oxygen species (ROS) than do fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This observation has led clinicians to treat infertile men with antioxidant supplements. The purpose of this article is to discuss the rationale for antioxidant therapy in infertile men and to evaluate the data on the efficacy of dietary and in vitro antioxidant preparations on sperm function and DNA damage. To date, most clinical studies suggest that dietary antioxidant supplements are beneficial in terms of improving sperm function and DNA integrity. However, the exact mechanism of action of dietary antioxidants and the optimal dietary supplement have not been established. Moreover, most of the clinical studies are small and few have evaluated pregnancy rates. A beneficial effect of in vitro antioxidant supplements in protecting spermatozoa from exogenous oxidants has been demonstrated in most studies; however, the effect of these antioxidants in protecting sperm from endogenous ROS, gentle sperm processing and cryopreservation has not been established conclusively. Keywords: antioxidant; male infertility; oxidative stress; sperm DNA fragmentation; vitamins RELATIONSHIP BETWEEN OXIDATIVE STRESS AND SPERM DYSFUNCTION Seminal oxidative stress (OS) results from an imbalance between reactive oxygen species (ROS) production and ROS scavenging by seminal antioxidants. Seminal OS is believed to be one of the main factors in the pathogenesis of sperm dysfunction and sperm DNA damage in male infertility.1-4 Indeed, it is estimated that 25% of infertile men possess high levels of semen ROS, whereas fertile men do not have high levels of semen ROS.1,4-6 Although a controlled production of these ROS is required for sperm physiology (sperm hyperactivation, capacitation and acrosome reaction) and for natural fertilization, [7][8][9] the excessive production of ROS by immature germ cells and leukocytes causes sperm dysfunction (lipid peroxidation, loss of motility and sperm DNA damage). 9,10 Spermatozoa are particularly susceptible to oxidative injury due to the abundance of plasma membrane polyunsaturated fatty acids. [10][11][12] These unsaturated fatty acids provide fluidity that is necessary for membrane fusion events (e.g., the acrosome reaction and sperm-egg interaction) and for sperm motility. However, the unsaturated nature of these molecules predisposes them to free radical attack and ongoing lipid peroxidation throughout the sperm plasma membrane. Once this process has been initiated, accumulation of lipid peroxides occurs on the sperm surface (this results in loss of sperm motility) and oxidative damage to DNA can ensue. 13,14SEMINAL ANTIOXIDANT CAPACITY AND SPERM DYSFUNCTION Seminal plasma and spermatozoa themselves are well endowed with an array of protective antioxidants to protect spermatozoa from OS, especially, at the post-testicular level. 6,15,16 Seminal plasma contains a number of high-molecular weigh...

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“…There is evidence that sperm of infertile men contain more DNA damage than fertile men and that this sperm DNA damage may have a negative effect on fertility potential of these patients [40,55,71,114,127,130]. While high levels of sperm DNA damage often correlate with poor seminal parameters such as reduced count and motility or abnormal morphology [65,80,92], sperm DNA damage is also found in 8% of men with normal seminal parameters [127,130]. Additionally, there have been concerns about potential consequences of the use of DNA damaged sperm in intracytoplasmic sperm injection (ICSI) as this technique overrides the process of natural selection [109].…”

Section: Introductionmentioning

confidence: 99%

Sperm DNA damage in male infertility: etiologies, assays, and outcomes

Schulte

Ohl

Sigman³

et al. 2009

J Assist Reprod Genet

227

159

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Male factor infertility is the sole cause of infertility in approximately 20% of infertile couples, with an additional 30% to 40% secondary to both male and female factors. Current means of evaluation of male factor infertility remains routine semen analysis including seminal volume, pH, sperm concentration, motility, and morphology. However, approximately 15% of patients with male factor infertility have a normal semen analysis and a definitive diagnosis of male infertility often cannot be made as a result of routine semen analysis. Attention has focused on the role of sperm nuclear DNA integrity in male factor infertility. Here we review the structure of human sperm chromatin, the etiology and mechanisms of sperm DNA damage, current tests available to assess sperm DNA integrity, and effect of sperm DNA integrity on reproductive outcomes.

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Biologic variability of sperm DNA denaturation in infertile men

Cited by 120 publications

References 13 publications

Sperm Dna Integrity: Correlation With Sperm Plasma Membrane Integrity In Semen Evaluated For Male Infertility

Sperm Dna Integrity: Correlation With Sperm Plasma Membrane Integrity In Semen Evaluated For Male Infertility

Antioxidant therapy in male infertility: fact or fiction?

Sperm DNA damage in male infertility: etiologies, assays, and outcomes

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