Biological activation of bone grafts using injectable platelet-rich fibrin

Shah, Radhika; Gowda, Triveni M; Thomas, Raison; Kumar, Tarun; Mehta, Dhoom Singh

doi:10.1016/j.prosdent.2018.03.027

Cited by 35 publications

(45 citation statements)

References 11 publications

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“…The i-PRF is collected from the upper liquid layer [ 17 ], which is not clearly separated into three layers as with a PRF membrane. A study collected the plasma 2 mm above the junction for i-PRF [ 5 ], while others used the plasma from as close as possible to the red blood cell layer [ 9 , 18 ]. Mourao et al found that there is the intermediate orange layer, which contained more platelets and white blood cells [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…Platelet-rich fibrin (PRF), introduced by Choukroun et al [ 4 ], is an autogenous fibrin product that, unlike platelet-rich plasma, can be obtained from blood using low-speed centrifugation without adding anticoagulants. The benefits of using PRF for bone regeneration include that PRF functions as a scaffold for endogenous cell homing and as a signaling protein reservoir for osteoinduction [ 5 ]. PRF was initially used as a membrane, which enhanced soft tissue healing rather than bone regeneration [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Red and Yellow Injectable Platelet-Rich Fibrin Demonstrated Differential Effects on Periodontal Ligament Stem Cell Proliferation, Migration, and Osteogenic Differentiation

Thanasrisuebwong

Kiattavorncharoen

Surarit

et al. 2020

IJMS

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The biological benefits of using two fractions derived from injectable platelet-rich fibrin (i-PRF) in bone regeneration remain unclear. Thus, the current study examined two fractionation protocols producing yellow i-PRF and red i-PRF on periodontal ligament stem cells (PDLSCs). The i-PRF samples from five donors were harvested from two different levels, with and without a buffy coat layer, to obtain red and yellow i-PRF, respectively. The PDLSCs were isolated and characterized before their experimental use. The culture medium in each assay was loaded with 20% of the conditioned medium containing the factors released from the red and yellow i-PRF. Cell proliferation and cell migration were determined with an MTT and trans-well assay, respectively. Osteogenic differentiation was investigated using alkaline phosphatase and Alizarin red staining. The efficiency of both i-PRFs was statistically compared. We found that the factors released from the red i-PRF had a greater effect on cell proliferation and cell migration. Moreover, the factors released from the yellow i-PRF stimulated PDLSC osteogenic differentiation earlier compared with the red i-PRF. These data suggest that the red i-PRF might be suitable for using in bone regeneration because it induced the mobilization and growth of bone regenerative cells without inducing premature mineralization.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Red and Yellow Injectable Platelet-Rich Fibrin Demonstrated Differential Effects on Periodontal Ligament Stem Cell Proliferation, Migration, and Osteogenic Differentiation

Thanasrisuebwong

Kiattavorncharoen

Surarit

et al. 2020

IJMS

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“…An initial review of the literature highlighted the great variety of platelet-derived products and the heterogeneity in their nomenclature and processing methods, with confusion over their therapeutic properties and suggested applications [18][19][20][21]. Most protocols of PRP preparation are very similar to each other: the withdrawal of autologous blood in the presence of an anticoagulant; its centrifugation with plasma separation; leukocyte inclusion or exclusion; and the activation of platelet with different stimuli.…”

Section: Discussionmentioning

confidence: 99%

The Number of Platelets in Patient’s Blood Influences the Mechanical and Morphological Properties of PRP-Clot and Lysophosphatidic Acid Quantity in PRP

Bosetti

Boffano

Marchetti

et al. 2019

IJMS

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The objectives of this study were to compare platelet-rich plasma (PRP) from patients with different concentrations of platelets and to assess the influence of these PRP preparations on human osteoblast (hOB) activity. In the literature, growth factors released by activated platelets have been considered responsible for the active role of PRP on bone regeneration but no specific role has been attributed to lysophosphatidic acid (LPA) as a possible effector of biological responses. In this study, patients were grouped into either group A (poor in platelets) or group B (rich in platelets). Clots from PRP fraction 2 (F2-clots), obtained with CaCl2 activation of PRP from the two groups, were compared macroscopically and microscopically and for their mechanical properties before testing their activity on the proliferation and migration of hOB. LPA was quantified before and after PRP fractioning and activation. The fibrin network of F2-clots from patients with a lower platelet concentration had an organized structure with large and distinct fibers while F2-clots from patients in group B revealed a similar structure to those in group A but with a slight increase in density. ELISA results showed a significantly higher plasma level of LPA in patients with a higher platelet concentration (group B) in comparison to those in group A (p < 0.05). This different concentration was evidenced in PRP but not in the clots. Depending on the number of platelets in patient’s blood, a PRP-clot with higher or lower mechanical properties can be obtained. The higher level of LPA in PRP from patients richer in platelets should be considered as responsible for the higher hOB activity in bone regeneration.

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“…The reasons for the excluded records are summarised in Table A4 [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39].…”

Section: Selection Of the Studiesmentioning

confidence: 99%

“…Alhasyimi et al [24] Animal study Che et al [25] Review article Dukka et al [26] Case Report Janssen et al [27] Evaluates PRP and not PRF Stasiak et al [28] Systematic review Shetty et al [29] Trial registration Subbalekha et al [30] Trial registration Avinash et al [31] Trial registration Tehranchi et al [32] Trial registration Mazzone et al [33] Not orthodontic field Shah et al [34] Technical note Iskenderoglu et al [35] Case Report Dimofte et al [36] Review article Nadon et al [37] Evaluate a derivate of PRF Aras et al [38] Case Report Findik et al [39] Case Report…”

Section: Reason For Exclusionmentioning

confidence: 99%

Platelet-Rich Fibrin in Bone Regenerative Strategies in Orthodontics: A Systematic Review

2020

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Preservation of the alveolar bone is a determinant in the outcome of orthodontic treatment. Alveolar bone defects or a decrease of their height and width may occur due to common reasons such as inflammation, tooth extraction, or cleft lip and palate. The aim of this systematic review was to investigate and appraise the quality of the most up to date available evidence regarding the applications and effects of platelet-rich fibrin (PRF) in orthodontics. This study was carried out according to preferred reporting items for systematic reviews and meta-analyses guidelines using the following databases: Medline via PubMed, Cochrane Library, Web of Science Core Collection and EMBASE. The qualitative assessment of the included studies was performed using Cochrane Risk of Bias tool and ROBINS-I guidelines. Results: From a total of 489 studies, nine studies were selected. The majority of the included studies demonstrate that autogenous anterior iliac graft with PRF had a higher amount of newly formed bone. Furthermore, this review also suggests that the application of platelet derivatives in the extraction socket can accelerate orthodontic tooth movement. Despite the limitations in the included studies, this systematic review suggested that PRF can improve alveolar cleft reconstruction and orthodontic tooth movement.

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Biological activation of bone grafts using injectable platelet-rich fibrin

Cited by 35 publications

References 11 publications

Red and Yellow Injectable Platelet-Rich Fibrin Demonstrated Differential Effects on Periodontal Ligament Stem Cell Proliferation, Migration, and Osteogenic Differentiation

Red and Yellow Injectable Platelet-Rich Fibrin Demonstrated Differential Effects on Periodontal Ligament Stem Cell Proliferation, Migration, and Osteogenic Differentiation

The Number of Platelets in Patient’s Blood Influences the Mechanical and Morphological Properties of PRP-Clot and Lysophosphatidic Acid Quantity in PRP

Platelet-Rich Fibrin in Bone Regenerative Strategies in Orthodontics: A Systematic Review

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