Biological activation of zirconia surfaces by chemical modification with IGF‐1

Abstract

The purpose of this study was to improve the adhesion and extension of human gingival epithelial cells (HGECs) to the yttria-stabilized zirconia polycrystal (Y-TZP) surfaces by immobilization of insulin-like growth factor 1 (IGF-1). Surface analyses by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) showed that IGF-1 was successfully immobilized on the Y-TZP surfaces. There was no significant difference between the number of cells attached to the IGF-1-immobilized Y-TZP surfaces and on the as-polished Y-TZP surfaces either at 3 or 72 h. However, IGF-1-immobilized Y-TZP surfaces yielded a significantly higher expression of integrin β4 mRNA and laminin-5 mRNA, and enhanced adhesion strength of HGECs after 72 h of incubation. There was no difference between the amount of adhered Streptococcus gordonii (S. gordonii) found on the IGF-1-immobilized Y-TZP surfaces and on the as-polished Y-TZP surfaces. These results suggested that the IGF-1-immobilized Y-TZP surfaces developed using the method reported herein enhanced the adhesion and extension of HGECs to the Y-TZP surfaces without enhancing S. gordonii adhesion.

Self-assembling nanomaterial-based peptide surface for target cell adhesion

“…X-ray photoelectron spectroscopy (XPS) survey peaks to analyze the changes in the chemical and physical structure of the prepared bio-functional SPCE surfaces are given in Figure 1 [39][40][41][42][43].To obtain information about the morphological conditions of the prepared bio-functional SPCE surfaces, SEM images of prepared surfaces are given in Figure 2 for blank SPCE surface, SPCE surface after AuNP adsorption and SPCE surface after AuNP/GSH immobilization, SPCE surface after AuNP/Car immobilization, and SPCE surface after AuNP/IGF-1 immobilization.…”

“…Therefore, it suggests that the cysteine residues in its structure are attached not by adsorption but by its affinity for AuNP. It is a characteristic peak of 400 eV, and it has been stated that it is a peptide bond peak and is covalently bound to the surface [43].…”

Silk fibroin microgrooved zirconia surfaces improve connective tissue sealing through mediating glycolysis of fibroblasts

“…This technology facilitated the exact and programmable creation of patterned surfaces, thereby minimizing material waste [ 23 ]. The selection of bio-ink is vital for ensuring the biological effectiveness of the coated surface, with recent studies exploring the use of bioactive substances like platelet lysates and fibrinogen to enhance tissue compatibility of implant [ [24] , [25] , [26] , [27] , [28] ]. Despite their benefits, these materials often presented challenges related to immunogenicity and required extensive separation and purification processes, elevating costs and increasing the risk of infection [ 29 ].…”