Biological activities of new secondary metabolite produced by <em>Streptomyces badius</em>

Abstract

Aim of the study  Isolation and purification of a new metabolite fromStreptomyces badius ATCC19888 fermentation broth. Determination of chemical structure of isolated compound. Evaluation of its biological activities. Materials and MethodsStrain: Streptomyces badius ATCC 19888 was isolated from the soil in Kaukasus (Russia).Fermentation and purification: fermenter (Sartorius Biostat A, Germany), HPLC (Knauer). NMR analysis:The 1D 1 H and 13C NMR spectra as well as 2D homo-and heteronuclear spectra were collected on a 700MHz Bruker AVANCE III spectrometer, equipped with a QCI CryoProbe Experiments were performed at 25°C. Spectra were processed and prepared with TopSpin 3.0 Bruker Software. HR MS analysis: MaldiSYNAPT G2-S HDMS (Waters) coupled with ACQUITY UPLC I-Class System (Waters).