Biological activities of ribosome-inactivating proteins and their possible applications as antimicrobial, anticancer, and anti-pest agents and in neuroscience research

Akkouh, Ouafae; Ng, Tzi Bun; Cheung, Randy Chi Fai; Wong, Jack Ho; Pan, Wenliang; Ng, Charlene Cheuk Wing; Sha, Ou; Shaw, Pang Chui; Chan, Wai‐Yee

doi:10.1007/s00253-015-6941-2

Cited by 28 publications

(23 citation statements)

References 190 publications

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“…In agriculture, It is demonstrated in vitro and in transgenic plants that RIPs have been connected to defense by antifungal, antibacterial, antiviral and insecticidal activities ( Stevens et al, 1981 ; Wang and Tumer, 2000 ; Choudhary N.L. et al, 2008 ; Akkouh et al, 2015 ). These studies have conferred a wide knowledge base for comprehending the medicinal and biochemical properties of RIPs.…”

Section: Introductionmentioning

confidence: 99%

“…As shown in Figure 2 , the proposed model for the role of RIPs in defense against pathogens showed that various RIPs have shown unique bioactive properties, including antibacterial, antifungal, antiviral, and insecticidal activity ( Stirpe and Battelli, 2006 ; Shu et al, 2009 ; Akkouh et al, 2015 ). A large number of studies suggest that the expression of RIPs could be activated by some factors, such as viral infection, phytohormones, senescence, development, and environmental stress ( Reinbothe et al, 1994 ; Girbés et al, 1996 ; Stirpe et al, 1996 ; Rippmann et al, 1997 ; Wang et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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The Plant Ribosome-Inactivating Proteins Play Important Roles in Defense against Pathogens and Insect Pest Attacks

et al. 2018

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Ribosome-inactivating proteins (RIPs) are toxic N-glycosidases that depurinate eukaryotic and prokaryotic rRNAs, thereby arresting protein synthesis during translation. RIPs are widely found in various plant species and within different tissues. It is demonstrated in vitro and in transgenic plants that RIPs have been connected to defense by antifungal, antibacterial, antiviral, and insecticidal activities. However, the mechanism of these effects is still not completely clear. There are a number of reviews of RIPs. However, there are no reviews on the biological functions of RIPs in defense against pathogens and insect pests. Therefore, in this report, we focused on the effect of RIPs from plants in defense against pathogens and insect pest attacks. First, we summarize the three different types of RIPs based on their physical properties. RIPs are generally distributed in plants. Then, we discuss the distribution of RIPs that are found in various plant species and in fungi, bacteria, algae, and animals. Various RIPs have shown unique bioactive properties including antibacterial, antifungal, antiviral, and insecticidal activity. Finally, we divided the discussion into the biological roles of RIPs in defense against bacteria, fungi, viruses, and insects. This review is focused on the role of plant RIPs in defense against bacteria, fungi, viruses, and insect attacks. The role of plant RIPs in defense against pathogens and insects is being comprehended currently. Future study utilizing transgenic technology approaches to study the mechanisms of RIPs will undoubtedly generate a better comprehending of the role of plant RIPs in defense against pathogens and insects. Discovering additional crosstalk mechanisms between RIPs and phytohormones or reactive oxygen species (ROS) against pathogen and insect infections will be a significant subject in the field of biotic stress study. These studies are helpful in revealing significance of genetic control that can be beneficial to engineer crops tolerance to biotic stress.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Plant Ribosome-Inactivating Proteins Play Important Roles in Defense against Pathogens and Insect Pest Attacks

et al. 2018

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“…However, unlike plant lectins , none of the mushroom lectins isolated to date displays antifungal activity. Fungal ribosome-inactivating proteins and their plant counterparts (Stirpe andBattelli, 2006, Stirpe 2013) have dissimilar N-terminal sequences but have biological activities in common like antimicrobial, anticancer and antiviral activities (Akkouh et al 2015). Fungal ribonucleases display anticancer and HIV-1 reverse transcriptase activities like ribonucleases of other origins (Fiorini et al, 2014(Fiorini et al, , 2015.…”

Section: Resultsmentioning

confidence: 99%

Fungal proteinaceous compounds with multiple biological activities

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Wong

et al. 2016

Appl Microbiol Biotechnol

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Fungi comprise organisms like molds, yeasts and mushrooms. They have been used as food or medicine for a long time. A large number of fungal proteins or peptides with diverse biological activities are considered as antibacterial, antifungal, antiviral and anticancer agents. They encompass proteases, ribosome inactivating proteins, defensins, hemolysins, lectins, laccases, ribonucleases, immunomodulatory proteins, and polysaccharopeptides. The target of the present review is to update the status of the various bioactivities of these fungal proteins and peptides and discuss their therapeutic potential.

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“…MAP30 has been shown to inhibit HIV; it also inhibits the cell proliferation of a panel of tumor cells in vitro and in vivo (Fang and Ng 2011; Fang et al 2012a, b). Recent studies have also shown that MAP30 can inhibit the herpes simplex virus and pathogenic fungus (Akkouh et al 2015; Wang et al 2016), and could induce cell death (Fan et al 2015). Taken together, the results of previous studies have suggested that the anti-viral activities are the most characterized of MAP30, especially activities against HIV, HBV, and tumor cell proliferation (Lee-Huang et al 1995; Fan et al 2008, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The results demonstrated that non-glycosylated MAP30 protein is as active as the glycosylated natural protein, and recombinant MAP30 possesses anti-HIV, anti-HBV, anti-tumor and apoptosis induction activities (Lee-Huang et al 1995; Fan et al 2008, 2009). Recent studies have also revealed that MAP30 exerts activities against the herpes simplex virus and pathogenic fungus (Akkouh et al 2015; Wang et al 2016), and can induce cell-cycle arrest and apoptosis in human lung carcinoma cells (Fan et al 2015). However, the effects of recombinant MAP30 in terms of antibacterial activities and possible replacement of antibiotics are unknown, and the toxicity and immunogenicity of MAP30 have also never been studied.…”

Section: Introductionmentioning

confidence: 99%

Novel purification method and antibiotic activity of recombinant Momordica charantia MAP30

et al. 2017

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Ribosome-inactivating proteins (RIPs) are a group of enzymes originally isolated from plants that possess the ability to damage ribosomes in an irreversible manner, leading to inhibition of protein synthesis in eukaryotic cells. In this study, we aimed to purify recombinant RIPs, investigate their function in the treatment of bacterial infection, and determine their toxicity in mice. We employed a pMAL protein fusion and purification system using E. coli transformed with a plasmid containing MBP-tagged MAP30 cDNA. MBP-tagged MAP30 was purified using a modified novel protocol to effectively produce highly active MAP30 of high purity. In an acute toxicity study in mice, no mortality occurred at doses lower than 1.25 mg/kg. MAP30 at both 0.42 and 0.14 mg/kg induced anti-MAP30 IgG, which reached a maximum titer at week 3. In conclusion, recombinant MAP30 prepared using our purification method possesses bioactivity, and has a synergistic bacteria-killing effect that can significantly reduce the required dosages of chloramphenicol and erythromycin. Therefore, when MAP30 is used in combination with chloramphenicol or erythromycin, it may of benefit in terms of reducing the side effects of the antibiotics, as lower concentrations of antibiotics are required.

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Biological activities of ribosome-inactivating proteins and their possible applications as antimicrobial, anticancer, and anti-pest agents and in neuroscience research

Cited by 28 publications

References 190 publications

The Plant Ribosome-Inactivating Proteins Play Important Roles in Defense against Pathogens and Insect Pest Attacks

The Plant Ribosome-Inactivating Proteins Play Important Roles in Defense against Pathogens and Insect Pest Attacks

Fungal proteinaceous compounds with multiple biological activities

Novel purification method and antibiotic activity of recombinant Momordica charantia MAP30

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