“…This ABTS free-radical-scavenging assay revealed similar results to those obtained from the DPPH free-radical-scavenging assay [ 30 ]. The finding that DT extracted with 50% ethanol exhibited better DPPH and ABTS free-radical-scavenging abilities than DT extracted with water was consistent with previous findings for Dendrobium flower extracts [ 31 ].…”
This study investigated the polyphenol content, antioxidant activity, and inhibition ability of mushroom tyrosinase and melanogenesis of Dendrobium tosaense (DT) extract. Ground DT was extracted using deionized water (W) or 50% ethanol (50E) at room temperature (RT) or 50 °C (50T) for 20 min. The 50T + 50E extract exhibited the highest total phenol content 47.0 ± 4.0 mg gallic acid equivalent/g DT extract, the highest level of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging 66.0 ± 3.0 mg Trolox equivalent/g DT extract, and the highest reducing power 12.00 ± 0.50 mg vitamin C equivalent/g DT extract. The RT + W extract had the highest total flavonoid content 110.0 ± 3.0 mg quercetin equivalent/g DT extract. The RT + 50E extract had the lowest half maximal inhibitory concentration 1.30 ± 0.00 mg/mL for 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging, and the lowest half maximal inhibitory concentration 6.40 ± 0.30 mg/mL for mushroom tyrosinase inhibition activity. DT extracts, especially RT + W and 50T + W, exhibited potent inhibitory effects on melanogenesis of B16/F10 cells. These results demonstrated the application potential of DT extract for skincare.
“…This ABTS free-radical-scavenging assay revealed similar results to those obtained from the DPPH free-radical-scavenging assay [ 30 ]. The finding that DT extracted with 50% ethanol exhibited better DPPH and ABTS free-radical-scavenging abilities than DT extracted with water was consistent with previous findings for Dendrobium flower extracts [ 31 ].…”
This study investigated the polyphenol content, antioxidant activity, and inhibition ability of mushroom tyrosinase and melanogenesis of Dendrobium tosaense (DT) extract. Ground DT was extracted using deionized water (W) or 50% ethanol (50E) at room temperature (RT) or 50 °C (50T) for 20 min. The 50T + 50E extract exhibited the highest total phenol content 47.0 ± 4.0 mg gallic acid equivalent/g DT extract, the highest level of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging 66.0 ± 3.0 mg Trolox equivalent/g DT extract, and the highest reducing power 12.00 ± 0.50 mg vitamin C equivalent/g DT extract. The RT + W extract had the highest total flavonoid content 110.0 ± 3.0 mg quercetin equivalent/g DT extract. The RT + 50E extract had the lowest half maximal inhibitory concentration 1.30 ± 0.00 mg/mL for 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging, and the lowest half maximal inhibitory concentration 6.40 ± 0.30 mg/mL for mushroom tyrosinase inhibition activity. DT extracts, especially RT + W and 50T + W, exhibited potent inhibitory effects on melanogenesis of B16/F10 cells. These results demonstrated the application potential of DT extract for skincare.
“…Dendrobiums species have been thought as one of the most valuable medicinal genus in folk medicine and industrial crop with excellent medicinal merits, such as clearing unhealthy heat, nourishing Yin, benefiting the stomach, enhancing the body’s immunity, resisting cancer, and prolonging life (Ge et al 2018; Zha et al 2017; Zhao et al 2017). And they have long been extensively used as the popular tonic and traditional Chinese medicines (TCMs) in many Asian countries (Chinese Pharmacopoeia Committee 2015), as well as a high-quality health food in South and Southeast Asia for hundreds of years (Kanlayavattanakul et al 2018; Zhu et al 2009).…”
Owing to their significant medicinal and edible values, the natural
Dendrobium
species have underdone over-collection and habitat destruction, and cultivated species emerged for candidates. However, these
Dendrobium
plants are similar in shape to be easily confused, leading to extreme difficulties for identification based on their morphological and chemical features. In this study, the rDNA ITS region sequence analysis was developed for rapid and accurate identification of thirteen wild and cultivated
Dendrobium
species belonging to two sections
Formosae
and
Chrysotoxae
. By cloning and sequencing the rDNA ITS region genes from 13
Dendrobium
species, the phylogenetic relationships among them were analyzed. Results showed that the variation of the ITS region, together with the lengths and Guanine and Cytosine contents of ITS, 5.8s rDNA, ITS1 and ITS2 sequences occurred in the tested
Dendrobium
species, and which from section
Chrysotoxae
was higher than that from section
Formsae
. Phylogenetic analysis based on neighbor-joining and maximum p-arsimony trees indicated that the
Dendrobium
species of sections
Formosae
and
Chrysotoxae
could be well divided into two groups. A majority of
Dendrobium
species exhibited distinctive ITS2 secondary structures, while for those with close genetic relationships were similar. Therefore, the ITS2 region sequence analysis is simple, quick, and highly reliable that can be used as an effective tool for molecular identification and classification, as well as the reconstruction of the phylogeny of wild and cultivated
Dendrobium
species belonging to different sections.
“…Statistically, ChPg extract also showed the greatest ability to neutralize DPPH free radicals among all of the extracts ( p < 0.05). Compounds with activity against free radicals and oxidative stress are a new strategy in combating aging and skin hyperpigmentation (Kanlayavattanakul et al., 2018). Figure 2 In vitro antioxidant activities (a) and tyrosinase inhibition activities (b) of I. javanica flower extracts obtained with various DESs.…”
The high demand for cosmetics has had a great impact on the development of innovative products in the cosmetic industry. The availability of raw materials has become a common problem in the cosmetic industry. Materials from nature can act as alternative sources, such as Ixora javanica. Several studies have shown the potential of I. javanica as an antioxidant and skin lightening agent. The objectives of the present study were to develop and optimize a green ultrasound-assisted deep eutectic solvent extraction of I. javanica. Eleven deep eutectic solvents were evaluated based on extraction efficiency parameters; that is, flavonoid and anthocyanin yields; the antioxidant and tyrosinase inhibitory activities of the extracts. The combination of choline chloride and propylene glycol (1:1) was shown to be the optimal deep eutectic solvent for I. javanica extraction. The extraction parameters of temperature, extraction time, and solid-to-liquid ratio were also optimized using response surface methodology. The total flavonoid compound obtained was 33 mg quercetin equivalent/g dried sample under the optimum extraction condition (extraction time of 5 min, temperature of 57 °C, solid-to-liquid ratio of 0.02 g/mL). In sum, this work demonstrates the potential of natural deep eutectic solvent as an organic solvent replacement to obtain high quality Ixora javanica extract, which is a potential new source of skin-lightening cosmetic materials.
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