Although softwoods are widely distributed in Korea and used in various industries, studies are limited and comparative research on softwoods has been overlooked. Therefore, in this study, the physiological activity of four species of softwoods found in Korea was quantified. Needles and cones of Pinus densiflora, Chamaecyparis obtusa, Cryptomeria japonica, and Abies koreana were collected, and material was extracted using 70% methanol. We quantified the following physiological traits: total phenol and flavonoid content, antioxidant activity (DPPH, TEAC, FRAP), and inhibitory activity of the enzymes α-glucosidase, elastase, and tyrosinase. Total phenol and flavonoid contents and antioxidant activities were high in Chamaecyparis obtusa and Cryptomeria japonica, and α-glucosidase inhibition activity was highest in the leaves of Chamaecyparis obtusa. Elastase inhibitory activity was high in the leaves and cones of Pinus densiflora and the cones of Abies koreana, and tyrosinase inhibitory activity was highest in the leaves of Pinus densiflora. In Korea, softwoods are planted extensively on roadsides and as windbreaks, and they are highly competitive in supplying raw materials since they are pruned every year. Based on the results of this study, softwoods are considered suitable for use in healthcare products, diabetes products, wrinkles, and whitening cosmetics.