This study aimed to evaluate the anti-adipogenic and anti-inflammation effects of
extract from Maclura tricuspidata twig fermented with
Ganoderma lucidum mycelium (EMFG) in 3T3-L1 preadipocytes.
3T3-L1 adipocytes were treated with 100, 200, 300 μg/mL of EMFG. The
result showed that EMFG dose-dependently inhibited the accumulation of
intracellular lipid content in differentiated 3T3-L1 adipocytes and enhanced
increase of adiponectin release and inhibition of leptin release. EMFG treatment
reduced expression of adipogenic transcriptional factor such as peroxisome
proliferator-activated receptor γ (PPARγ), CCAAT-enhancer-binding
protein α (C/EBPα). EMFG also decreased production of
lipopolysaccharide (LPS)-induced inflammatory cytokine [tumor necrosis
factor-α (TNF-α), interleukin-6 (IL-6) and monocyte
chemoattractant protein-1 (MCP-1)] and the protein expression of
cyclooxygenase-2 (COX-2) and inducible NOS (iNOS) in differentiated 3T3-L1
adipocytes. The study demonstrated that EMFG inhibited adipogenesis and
inflammation in a dose-dependent manner. These findings suggest that EMFG may
have potential as an anti-obesity and anti-metabolic disease agent that works by
inhibiting adipogenesis and inflammation.