Biological activity of surfactins - a case of a biosurfactant produced by Bacillus subtilis PCM 1949

Siwak, Emilia; Jewgiński, Michał; Kustrzeba−Wójcicka, Irena

doi:10.18388/abp.2015_1149

Cited by 3 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, the I‐TASSER analysis clearly confirmed that the sequenced NRPS PCR products was more resemble to SRFA‐C gene which indicates the presence of SRFA‐C gene in the Nocardiopsis sp AJ1 genome, which responsible for the production of surfactin. Surfactin is formed by non‐ribosomal peptide synthetases (NRPSs), which govern all necessary steps in this process .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial potential of haloalkaliphilic Nocardiopsis sp. AJ1 isolated from solar salterns in India

et al. 2019

View full text Add to dashboard Cite

Antagonistic haloalkaliphilic Nocardiopsis sp. AJ1 (GenBank JX575136.1), isolated and identified from the saline soil of Kovalam solar salterns was able to produce antimicrobial secondary metabolites and effectively suppressed several bacterial and fungal pathogens. The metabolite extracted from ethyl acetate precipitation suppressed the bacterial and fungal pathogens to the range between 2.14 and 20.14 mm and also controlled the shrimp killer virus WSSV by 83% than the control and significantly (p < 0.05) differed. GC-MS analysis revealed that, the ethyl acetate precipitation contains pyrrolo (1,2-A(pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-) and actinomycin C2. Non ribosomal peptide synthetase (NRPS) was amplified by PCR with the amplicon size of 750-800 bp length and further predicted the secondary structure by Iterative Threading Assembly Refinement (I-TASSER) bioinformatics approach. I-TASSER prediction helped to find out the secondary, 3-D structure, and ligand binding sites. The top ten modelling concluded that, the NRPS gene is closely similar to surfactin synthesizing gene, surfactin A synthetase C (SRFA-C). The findings revealed that, the active compounds from the secondary metabolites effectively suppressed the pathogenic bacteria, fungi, and virus and useful to develop antimicrobials. K E Y W O R D S actinobacteria, antimicrobials, haloalkaliphils, Nocardiopsis sp. AJ1, non ribosomal peptide synthetase (NRPS)

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial potential of haloalkaliphilic Nocardiopsis sp. AJ1 isolated from solar salterns in India

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The ability to produce surfactin is a property of bacteria from the genus Bacillus, and since the discovery of surfactin in 1968 by Arima, this amphipathic lipopeptide has been found to possess several properties (66). Within these properties are those general to all lipopeptides and antibacterial proteins which act upon Gram-positive and Gram-negative bacteria (67,68), and other anti-inflammatory (69) and anti-viral effects (70).…”

Section: Differences In Prokaryote Interaction Pathwaysmentioning

confidence: 99%

Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics

et al. 2020

View full text Add to dashboard Cite

Purpose: To analyze human and bacteria proteomic profiles in bile, exposed to a tumor vs. non-tumor microenvironment, in order to identify differences between these conditions, which may contribute to a better understanding of pancreatic carcinogenesis. Patients and Methods: Using liquid chromatography and mass spectrometry, human and bacterial proteomic profiles of a total of 20 bile samples (7 from gallstone (GS) patients, and 13 from pancreatic head ductal adenocarcinoma (PDAC) patients) that were collected during surgery and taken directly from the gallbladder, were compared. g:Profiler and KEGG (Kyoto Encyclopedia of Genes and Genomes) Mapper Reconstruct Pathway were used as the main comparative platform focusing on over-represented biological pathways among human proteins and interaction pathways among bacterial proteins. Results: Three bacterial infection pathways were over-represented in the human PDAC group of proteins. IL-8 is the only human protein that coincides in the three pathways and this protein is only present in the PDAC group. Quantitative and qualitative differences in bacterial proteins suggest a dysbiotic microenvironment in the PDAC group, supported by significant participation of antibiotic biosynthesis enzymes. Prokaryotes interaction signaling pathways highlight the presence of zeatin in the GS group and surfactin in the PDAC group, the former in the metabolism of terpenoids and polyketides, and the latter in both metabolisms of terpenoids, polyketides and quorum sensing. Based on our findings, we propose a bacterial-induced carcinogenesis model for the biliary tract. Conclusion: To the best of our knowledge this is the first study with the aim of comparing human and bacterial bile proteins in a tumor vs. non-tumor microenvironment. We proposed a new carcinogenesis model for the biliary tract based on bile metaproteomic findings. Our results suggest that bacteria may be key players in biliary tract carcinogenesis, in a long-lasting dysbiotic and epithelially harmful microenvironment, in which specific bacterial species' biofilm formation is of utmost importance. Our finding should be further explored in future using in vitro and in vivo investigations.

show abstract

“…Thus, because pesticide use becomes less socially and ecologically acceptable [ 8 ], the development of biological methods of crop protection, like the use of beneficial microorganisms (biopesticides), will be a benefit and one of the most promising methods for more rational and safe crop management practices [ 9 ]. Biopesticides are important for reducing the risk of resistance to chemical pesticides [ 10 ], and the majority of them are biodegradable and less toxic to humans [ 11 ] and decompose faster, resulting in lower exposure [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Two Novel Bacillus Strains (subtilis and simplex Species) with Promising Potential for the Biocontrol of Zymoseptoria tritici, the Causal Agent of Septoria Tritici Blotch of Wheat

Allioui

Driss

Dhouib

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

Two novel Algerian field-collected isolates were selected for their antifungal activity against Zymoseptoria tritici (teleomorph Mycosphaerella graminicola). The novel strains, termed Alg.24B1 and Alg.24B2, were identified as Bacillus subtilis and Bacillus simplex since their respective nucleotide sequences of the 16S rRNA gene were 100% and 99.93% identical to those of B. subtilis and B. simplex, respectively. The antifungal activities of Alg.24B1 and Alg.24B2 were evaluated by the well diffusion method and compared to those of other Bacillus species. The maximum activity was obtained after two days of confrontation of the bacterial strain supernatants with the fungus for Alg.24B1 and three days for Alg.24B2. Furthermore, the metabolites responsible for the antifungal activity of both strains were detected by the investigation of either gene presence (PCR) or molecule production (activity detection of lytic enzymes and HPLC detection of lipopeptides). Overall, this study showed that in addition to their ability to produce lytic enzymes (protease and β-glucanase), both strains coproduce three types of lipopeptides viz. surfactin, iturin, and fengycin. Thus, the biofungicide activity of both strains may be a result of a combination of different mechanisms. Therefore, they had a great potential to be used as biocontrol agents to effectively manage septoria tritici blotch of wheat (STB).

show abstract

Biological activity of surfactins - a case of a biosurfactant produced by Bacillus subtilis PCM 1949

Cited by 3 publications

References 14 publications

Antimicrobial potential of haloalkaliphilic Nocardiopsis sp. AJ1 isolated from solar salterns in India

Antimicrobial potential of haloalkaliphilic Nocardiopsis sp. AJ1 isolated from solar salterns in India

Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics

Two Novel Bacillus Strains (subtilis and simplex Species) with Promising Potential for the Biocontrol of Zymoseptoria tritici, the Causal Agent of Septoria Tritici Blotch of Wheat

Contact Info

Product

Resources

About