Biological activity of the essential oils from Cinnamodendron dinisii and Siparuna guianensis

Abstract

This study had analyzed the antibacterial, antifungal and trypanocidal activity of the essential oils from Cinnamodendron dinisii Schwacke (Canellaceae) and Siparuna guianensis Aublet (Siparunaceae). The essential oils were obtained from fresh leaves by hydrodistillation, using a modified Clevenger apparatus. Chemical analysis by gas-liquid chromatography coupled to mass spectrometry (GC-MS) showed that these essential oils are rich in monoterpene and sesquiterpene hydrocarbons. Activity against the pathogenic bacteria Escherichia coli , Listeria monocytogenes , Pseudomonas aeruginosa , Salmonella choleraesuis and Staphylococcus aureus was evaluated with the agar cavity diffusion method, while activity on the filamentous fungi Aspergillus flavus , Aspergillus niger , Aspergillus carbonarius and Penicillium commune was evaluated by the disk diffusion technique. Trypanocidal activity was tested against Trypanosoma cruzi epimastigotes, using the Tetrazolium salt (MTT) colorimetric assay. Both essential oils exhibited low inhibitory effect towards bacteria, showing high MIC values (125–500 μg mL −1 ), with Gram positive bacteria being more susceptible. Better inhibitory effect was obtained for the evaluated fungi, with lower MIC values (7.81–250 μg mL −1 ), being A. flavus the most susceptible species. Both essential oils presented low trypanocidal activity, with IC 50 /24 h values of 209.30 μg mL −1 for S. guianensis and 282.93 μg mL −1 for C. dinisii . Thus, the high values observed for the MIC of evaluated bacteria and for IC 50 /24 h of T. cruzi , suggest that the essential oils have a low inhibitory activity against these microorganisms. In addition, the low MIC values observed for the tested fungi species indicate good inhibitory activity on these microorganisms’s growth.

Essential oils: in vitro activity against Leishmania amazonensis, cytotoxicity and chemical composition

“…The percentage of viable cells was determined by the equation [25]:Where %V is the percentage of viable cells, Fc: control fluorescence units; Fa: fluorescence units emitted by the analyzed samples (with inhibitor). The selectivity index (SI) was calculated by dividing CC 50 for the IC 50 .…”

Influence of seasonality on the yield and composition of the essential oil of Siparuna guianensis Aublet

“…Other ethnobotanical studies have revealed the traditional use of this plant for combating sinuses, headaches, migraines, and body aches, acting as an analgesic and anti-inflammatory agent (Valentini et al, 2010a;Andrade et al, 2015). It has also been reported to be used for fluctuation of consciousness between hypoactive and hyperactive states, neck pains, muscle strains, fears, vomiting, and seizures (Pagania et al, 2017).…”

“…The plant has also been evaluated for its activity against pathological bacteria such as Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella choleraesuis and Staphylococcus aureus; against the filamentous fungi such as Aspergillus flavus, Aspergillus niger, Aspergillus carbonarius, and Penicillium commune; and against the epimastigotes of Trypanosoma cruzi (Andrade et al, 2015). It has also been evaluated for its activity against the promastigotes of Leishmania amazonensis (Andrade et al, 2016) and as a repellent (Aguiar et al, 2015).…”

Antimicrobial activity and medicinal biomass of Siparuna guianensis in Brazilian Cerrado forest, a global hotspot

“…It´s a shrub that grows in the Cerrado and the aromatic leaves of S. guianensis are its largest reservoirs of essential oils and other chemical compounds of biological interest, such as flavonoids, tannins and terpenoids. In addition, the medicinal biomass (raw material) of the species has not been quantified, and there are few reports in the literature (Montanari, 2010;Valentini et al, 2010;Andrade et al, 2015) related to its potential antimicrobial activity, and no records of this approach in Tocantins State (277,720.520 km²), Brazil. In a recent study by Aguiar et al (2015), demonstrated highly toxic of essential oil of the S. guianensis against Aedes aegypti and Culex quinquefasciatus, showing potential for use as a natural insecticide against mosquitoes.…”

“…The medicinal species richness of the Cerrado is explained by the morphological characteristics of plants, which includes xylopodia and thick barks that accumulate reserves and frequently have active substances of pharmacological interest (Silva et al, 2010), including antibacterial and antifungal action (Pinhol et al, 2012;Silva et al, 2012;Andrade et al, 2015).…”