Biological Age of Cloned Animals

Saeki, Kazuhiro; Hoshino, Yoichiro; Taniguchi, Shunji

doi:10.1016/b978-0-12-386541-0.00033-3

Cited by 1 publication

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“…In recent years, other spectacular achievements have been made, such as cloning of mice from cells deriving from the skin tissue of a dead individual frozen without a cryoprotectant at -20° C for 16 years (Wakayama et al, 2008) or of a bull from cells obtained from testicles collected from a dead animal and frozen without a cryoprotectant at -80° C for 10 years (Hoshino et al, 2009). In turn, Saeki et al (2014), managed to obtain live cells from tissue fragments taken from the heart, ear, liver, kidneys and spleen that were held for 1 to 15 days at 4° C. Even cloning with cells recovered from beef purchased in a butcher's shop several days after slaughter (Adams et al, 2004;Arat et al, 2005) turned out to be equally spectacular, which indicates that even if an animal is dead but the karyoplast has not been damaged, the cells can be used for cloning. It creates the possibility of reproduction even if an animal is dead or extinct but whose tissues have been preserved in a frozen state.…”

Section: Remarkable Achievements With Different Sources Of Donor Cellsmentioning

confidence: 99%

Technical, Biological and Molecular Aspects of Somatic Cell Nuclear Transfer – A Review

Mrowiec

Bugno‐Poniewierska

2022

Annals of Animal Science

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Since the announcement of the birth of the first cloned mammal in 1997, Dolly the Sheep, 24 animal species including laboratory, farm, and wild animals have been cloned. The technique for somatic cloning involves transfer of the donor nucleus of a somatic cell into an enucleated oocyte at the metaphase II (MII) stage for the generation of a new individual, genetically identical to the somatic cell donor. There is increasing interest in animal cloning for different purposes such as rescue of endangered animals, replication of superior farm animals, production of genetically engineered animals, creation of biomedical models, and basic research. However, the efficiency of cloning remains relatively low. High abortion, embryonic, and fetal mortality rates are frequently observed. Moreover, aberrant developmental patterns during or after birth are reported. Researchers attribute these abnormal phenotypes mainly to incomplete nuclear remodeling, resulting in incomplete reprogramming. Nevertheless, multiple factors influence the success of each step of the somatic cloning process. Various strategies have been used to improve the efficiency of nuclear transfer and most of the phenotypically normal born clones can survive, grow, and reproduce. This paper will present some technical, biological, and molecular aspects of somatic cloning, along with remarkable achievements and current improvements.

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