Biological Analysis of a Candidate Stem Cell-KUSA/A1 cell-for Bone Tissue Engineering

Rodrı́guez, Augusto; Tsujigiwa, Hidetsugu; Borkosky, Silvina; Missana, Liliana Raquel; Sfer, Ana María; Gulsan, Sathi Ara; Gündüz, Esra; Lefeuvre, Mathieu; Takagi, Tohru; Nagai, Noriyuki

doi:10.2485/jhtb.16.1

Cited by 10 publications

(7 citation statements)

References 31 publications

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“…Because two moles of calcium ions quantitatively bind to a mole of Alizarin red S reagent,26 this staining method is useful in the identification of in vitro calcification with a high sensitivity. Previously, we have reported that KUSA/A1 cells are not capable of producing mineralized nodules cultured in alpha medium 23. Our observations demonstrated that CaTiO 3 –CaCO 3 , CaTiO 3 – a C, and HA induced calcification by KUSA/A1 cells cultured in alpha medium (Fig.…”

Section: Discussionsupporting

confidence: 59%

“…Previously, we have reported that KUSA/A1 cells are not capable of producing mineralized nodules cultured in alpha medium. 23 Our observations demonstrated that CaTiO 3 -CaCO 3 , CaTiO 3 -aC, and HA induced calcification by KUSA/ A1 cells cultured in alpha medium (Fig. 6).…”

Section: Discussionsupporting

confidence: 57%

“…To investigate the potential use of CaTiO 3 –CaCO 3 as a biomedical and dental material, we studied its influence on cell proliferation, osteoblastic differentiation, and mineralization on in vitro . In this study, we used bone marrow stromal cell line (KUSA/A1 cells),22 which is capable of maintaining an immature stage with high cell proliferation in alpha medium and shows good ability for osteogenesis/mineralization only in osteogenic medium 23. To maintain KUSA/A1 cells in an immature stage for evaluation of cell proliferation induced by different preparation of CaTiO 3 , the cells were cultured in alpha medium together with the particles (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Effect of CaTiO₃–CaCO₃ prepared by alkoxide method on cell response

Rodrı́guez¹,

Inoue²,

Tanaka³

et al. 2009

J Biomedical Materials Res

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In recent years, calcium titanate (CaTiO(3)) and carbon-containing materials have gained much attention in a number of biomedical material researches. To maximize the advantages of both materials, we developed a novel alkoxide method to get "calcium titanate with calcium carbonate" (CaTiO(3)-CaCO(3)). The objective was to evaluate the crystallinity and elemental composition of CaTiO(3)-CaCO(3) prepared by alkoxide method, CaTiO(3)-aC elaborated by modified thermal decomposition method, commercially-prepared CaTiO(3), and the effect of these materials on the bone marrow stromal cell. Hydroxyapatite was used as positive control material. We examined the cellular proliferation, osteoblastic differentiation, and mineralization of KUSA/A1 cells cultured with the materials. The results showed that CaTiO(3)-CaCO(3) and CaTiO(3)-aC contained evidence of calcium carbonate enhancing cell proliferation, osteoblastic differentiation, and mineralization. On the contrary, the commercially-prepared CaTiO(3) revealed absence of calcium carbonate with lower cell response than the other groups. The results indicated that calcium carbonate could play a key role in the cell response of CaTiO(3) material. In conclusion, our findings suggest that CaTiO(3)-CaCO(3) could be considered an important candidate as a biomaterial for medical and dental applications.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

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Effect of CaTiO₃–CaCO₃ prepared by alkoxide method on cell response

Rodrı́guez¹,

Inoue²,

Tanaka³

et al. 2009

J Biomedical Materials Res

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“…The KUSA-A1 mesenchymal cells were acquired from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan). KUSA-A1 is a bone marrow stromal stem cell line capable of differentiating into osteoblasts, chondrocytes, and myotubes under inducing conditions. , The KUSA-A1 cells were first cultured and incubated in an osteoblast-inducer medium consisting of 4.5 g/L of glucose DMEM ( d -glucose, l -glutamine, phenol red, and sodium pyruvate) supplemented with 10% fetal bovine serum. Cells were allowed to proliferate within Petri dishes for 24 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Silicon Nitride: A Bioceramic with a Gift

Pezzotti

2019

ACS Appl. Mater. Interfaces

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In the closing decades of the 20th century, silicon nitride (Si 3 N 4 ) was extensively developed for high-temperature gas turbine applications. Technologists attempted to take advantage of its superior thermal and mechanical properties to improve engine reliability and fuel economy. Yet, this promise was never realized in spite of the worldwide research, which was conducted at that time. Notwithstanding this disappointment, its use in medical applications in the early 21st century has been an unexpected gift. While retaining all of its engineered mechanical properties, it is now recognized for its peculiar surface chemistry. When immersed in an aqueous environment, the slow elution of silicon and nitrogen from its surface enhances healing of soft and osseous tissue, inhibits bacterial proliferation, and eradicates viruses. These benefits permit it to be used in a wide array of different disciplines inside and outside of the human body including orthopedics, dentistry, virology, agronomy, and environmental remediation. Given the global public health threat posed by mutating viruses and bacteria, silicon nitride offers a valid and straightforward alternative approach to fighting these pathogens. However, there is a conundrum behind these recent discoveries: How can this unique bioceramic be both friendly to mammalian cells while concurrently lysing invasive pathogens? This unparalleled characteristic can be explained by the pH-dependent kinetics of two ammonia speciesNH 4 + and NH 3 both of which are leached from the wet Si 3 N 4 surface.

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“…KUSA/A1 cells, a bone marrow stromal cell line, are capable of maintaining an immature stage when cultured in α-MEM even at high cell proliferation rates. Such a cell line solely induces mineralized nodules when cultured in specifi c osteogenic medium 29,30) . Thus, to determine CaTiO 3 -CaCO 3 effect on KUSA/A1 response, the cells were cultured in α-MEM together with the different compounds synthesized.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Efficacy of CaCO3 Content in CaTiO3– CaCO3 Composites for Bone Growth

Rodrı́guez

Sánchez

Felice

et al. 2018

J. Hard Tissue Biology.

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The eff ect of CaTiO 3 compounded with diff erent amounts of CaCO 3 on osteoblastic KUSA/A1 cells was evaluated. CaTiO 3 -CaCO 3 composites were obtained by alkoxide method, a simple, low-cost and reproducible technique used for largescale production of material. The content of CaCO 3 in our samples was controlled by varying the sintering time of the overall process. Composite morphology was assessed by scanning electron microscopy (SEM) showing particles with sizes ranging from100 to 500 nm. The presence of CaCO 3 was revealed by XRD and thermogravimetric analyses, which suggested that samples treated at 650ºC for 30 min contained higher amounts of CaCO 3 than samples treated for 2 and 10 h. Additionally, in vitro studies demonstrated that CaTiO 3 -CaCO 3 composites sintered for 30 min induced augmented cell proliferation and mineralization in comparison to composites sintered for longer periods of time. Hence, our fi ndings clearly suggest that the amount of CaCO 3 within CaTiO 3 -CaCO 3 composites exerts a critical eff ect on osteoblastic cells response. Enhanced bone regeneration could be achieved by increasing the content of CaCO 3 within the composites, thus establishing CaTiO 3 -CaCO 3 as a promising material for bone augmentation procedures in dental fi eld.

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Biological Analysis of a Candidate Stem Cell-KUSA/A1 cell-for Bone Tissue Engineering

Cited by 10 publications

References 31 publications

Effect of CaTiO₃–CaCO₃ prepared by alkoxide method on cell response

Effect of CaTiO₃–CaCO₃ prepared by alkoxide method on cell response

Silicon Nitride: A Bioceramic with a Gift

<i>In Vitro</i> Efficacy of CaCO<sub>3</sub> Content in CaTiO<sub>3</sub>– CaCO<sub>3</sub> Composites for Bone Growth

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Biological Analysis of a Candidate Stem Cell-KUSA/A1 cell-for Bone Tissue Engineering

Cited by 10 publications

References 31 publications

Effect of CaTiO3–CaCO3 prepared by alkoxide method on cell response

Effect of CaTiO3–CaCO3 prepared by alkoxide method on cell response

Silicon Nitride: A Bioceramic with a Gift

<i>In Vitro</i> Efficacy of CaCO<sub>3</sub> Content in CaTiO<sub>3</sub>– CaCO<sub>3</sub> Composites for Bone Growth

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Effect of CaTiO₃–CaCO₃ prepared by alkoxide method on cell response

Effect of CaTiO₃–CaCO₃ prepared by alkoxide method on cell response