Biological and chemical detection of fumonisins produced on agar medium by Fusarium verticillioides isolates collected from corn in Sohag, Egypt

Abstract

Fusarium verticillioides (Sacc.) Nirenberg is among the most common Fusarium species corn pathogens worldwide, and has been recognized as a fumonisin B 1 (FB 1 ) and fumonisin B 2 (FB 2 ) producer. In the present work, extracts of 58 F. verticillioides isolates from corn samples collected from Sohag Governorate, Egypt, were tested for their biotoxicity and production of fumonisin toxins. Forty-four Fusarium verticillioides isolates out of 58 tested produced FB 1 or FB 1 and FB 2 (15 and 29 isolates, respectively) on potato-sucrose agar medium, detected by TLC, whereas the other 14 isolates did not produce fumonisin toxins. HPLC crude extract analysis confirmed the results from TLC plates. Brine shrimp larvae as well as the Gram-negative bacteria Pseudomonas aeuroginosa showed low bio-sensitivity towards the F. verticillioides crude extract toxicity, whereas the Gram-positive bacteria Bacillus cereus and Bacillus subtilis, especially B. subtilis, showed higher sensitivity towards the tested Fusarium crude extracts. These results enabled us to bio-evaluate and chemically detect fumonisin mycotoxins using a simple agar medium technique.

Enzymatic and toxigenic ability of opportunistic fungi contaminating intensive care units and operation rooms at Assiut University Hospitals, Egypt

“…At the end of incubation periods, an agar plug technique was employed for the extraction of mycotoxins in culture materials, whereby, all the agar medium with fungal mycelia were cut into small pieces, transferred into a 250 ml Erlenmeyer flask containing 50 ml 96% methanol. The content was shaken on a rotary shaker (200 r.p.m., 24 h) and filtered through filter paper (Aboul-Nasr; Obied-Allah, 2013). …”

Identification of Pathogenic Fusarium spp. Causing Maize Ear Rot and Potential Mycotoxin Production in China

“…They are very often detected in cereal crops such as maize, wheat, and rice and have drawn wide attention owing to their toxicity and carcinogenicity. In 1973, Fusarium toxin was listed among the 16 research priorities of the World Health Organization and the Food and Agriculture Organization [ 7 , 8 , 9 , 10 ]. With improvements in living standards, higher requirements for food safety have been proposed.…”

Encapsulation of Apium graveolens essential oil into chitosan nanobiopolymer for protection of stored rice against Fusarium verticillioides and fumonisins contamination

“…The plates were viewed after spraying with p -anisaldehyde (0.5 g p -anisaldehyde in methanol-acetic acid-sulfuric acid; 8:10:5 v/v/v) under UV-trans-illuminator (Zenith Engineers, India) under short-wave (254 nm) and long-wave (365 nm) UV irradiation. The FB 1 and FB 2 appeared as slight bluish green spots with Rf value 0.25 and 0.30, respectively [ 24 , 25 ].…”