Biological and chemical diversity of cytotoxin-producing symbiotic marine fungi in intertidal zone of Dalian

Zhang, Yi; Mu, Jun; Feng, Yan; Dong, Xiaoli

doi:10.1007/s11434-012-5442-2

Cited by 8 publications

(5 citation statements)

References 37 publications

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“…Generally, microbial insecticides are considered as alternative to chemical insecticides, because of their selective toxicity, decomposability, and eco-friendly nature ( Misato, 1983 ; Shaalan et al, 2005 ). Mycelial extract of various fungi have been reported for their larvicidal, cellulolytic and cytotoxic activity ( Valentin Bhimba et al, 2011 ; Zhang et al, 2013 ; Fahd, 2018 ). The most commonly known fungal species which possess significant larvicidal activity are; Penicillium sp.…”

Section: Introductionmentioning

confidence: 99%

Larvicidal, Histopathological, Antibacterial Activity of Indigenous Fungus Penicillium sp. Against Aedes aegypti L and Culex quinquefasciatus (Say) (Diptera: Culicidae) and Its Acetylcholinesterase Inhibition and Toxicity Assessment of Zebrafish (Danio rerio)

et al. 2019

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Fungal metabolites are considered to be most efficient tools to overcome the issues related to insecticide resistance and environmental pollution. The present study focus on the evaluation of the mosquito larvicidal efficacy of metabolites of seven indigenous fungal isolates (Penicillium sp. Aspergillus niger, A. flavus, A. parasiticus, Rhizopus sp. Mucor sp. and Aspergillus sp.) on the larvae of Aedes aegypti and Culex quinquefasciatus under the laboratory condition. The preliminary screening of the isolate, Penicillium sp. showed better larvicidal effect when compared to other fungi. The fungus was grown on Potato Dextrose Broth (PDB) in the laboratory (at 25°C) and maintained in the relative humidity (at 76 ± 4% for 15 days). Larvicidal potency of mycelial ethyl acetate extract (MEAE) of Penicillium sp. was performed against 1st to 4th instars larvae of Ae. aegypti and Cx. quinquefasciatus using four different concentrations (100, 200, 300, and 500 μg/ml) that showed better larval mortality values (μg/ml) of LC50 = 6.554, 5.487, 6.874, 6.892, and the LC90 = 11.486, 10.366, 12.879, 13.865 for Ae. aegypti and LC50 = 7.000, 13.943, 18.129, 25.212 and the LC90 = 12.541, 23.761, 30.923, 41.696 for Cx. quinquefasciatus. Exposure of metabolite to larvae resulted in behavior changes i.e., excitation, up and down with aggressive movement, anal papillae biting behavior. Further, the larvae treated with Penicillium sp. metabolite exhibited significant reduction in the levels of acetylcholinesterase. The 4th instar mosquito larvae treated with the 500 μg/ml mycelia extract showed severe histological damages. During the antibacterial analysis of Penicillium sp.- mycelium the maximum growth inhibition zone was recorded in Shigella dysenteriae (31.2 mm) and Klebsiella pneumoniae (31.1 mm) followed by others. In addition, to check the toxicity of Penicillium sp. MEAE against embryos of Zebrafish, a model system, using different concentrations of metabolites (1.0, 0.5, 0.125 mg/ml, 30, 3.0, and 0.5 μg/ml) and life-stage parameters were observed at 124 hpf. Furthermore, the Fourier Transformed Infrared and GCMS spectrum analysis of mycelium reflected several chemical compounds. The outcome of the study clearly shows that Penicillium sp. metabolites could serve as an ideal eco-friendly, single-step and inexpensive source for the control of Ae. aegypti and Cx. quinquefasciatus larvae.

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Section: Introductionmentioning

confidence: 99%

Larvicidal, Histopathological, Antibacterial Activity of Indigenous Fungus Penicillium sp. Against Aedes aegypti L and Culex quinquefasciatus (Say) (Diptera: Culicidae) and Its Acetylcholinesterase Inhibition and Toxicity Assessment of Zebrafish (Danio rerio)

et al. 2019

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“…The Fungal based products are biodegradable and exhibited high toxicity against mosquitoes, with relatively lesser toxicity towards nontarget organisms, these characteristic features make them ideal eco-friendly tools which can be exploited for mosquito vector control (Kirschbaum 1985; Shaalan et al 2005a, b). Mycelial extracts of various fungi have been reported for their larvicidal, cellulolytic, and cytotoxic activity (Valentin Bhimba et al 2011; Zhang et al 2013;Fahd, 2018).…”

Section: Introductionmentioning

confidence: 99%

Novel development of Lecanicillium lecanii-based granules as a platform against malarial vector Anopheles culicifacies

Sogan

Kala

Kapoor

et al. 2023

World J Microbiol Biotechnol

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Mosquitoes are infectious vectors of a wide range of pathogens and parasites thereby transmitting several diseases like Malaria, Dengue, Chikungunya, Zika, and Japanese Encephalitis posing a major public health concern. Mostly synthetic insecticides are usually applied as a primary control strategy to manage vector-borne diseases. However excessive and non-judicious usage of such chemically derived insecticides has led to serious environmental and health issues owing to their biomagni cation ability and increased toxicity towards non-target organisms. In this context, many such bioactive compounds originating from entomopathogenic microbes may serve as an alternative and environmentally benign tool for vector control. In the present paper, the entomopathogenic fungus Lecanicillium lecanii was processed to make the granules. Developed 4% LL granules have been characterized using the technique of Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). The developed formulation was also subjected to an accelerated temperature study at 40° C and was found to be stable for 3 months. Further, GCMS of the L. lecanii was also performed to screen the potential biomolecules present. The developed formulation was found to be lethal against Anopheles culicifacies with an LC 50 value of 11.836 µg/mL. The ndings from SEM and histopathology also substantiated the mortality effects. Further SEM EDX studies revealed that treated mosquitoes have lower nitrogen content which is correlated to a lower level of chitin whereas the control one has higher chitin content and healthy membranes. The developed LL granule formulation exhibited high toxicity against Anopheles mosquitoes due to its selective toxicity, thereby minimizing the toxicity toward non-target organisms. The granule formulations can be used as an effective biocontrol strategy against malaria-causing mosquitoes.

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Section: Introductionmentioning

confidence: 99%

Novel development of Lecanicillium lecanii-based granules as a platform against malarial vector Anopheles culicifacies

Sogan

Kala

Kapoor

et al. 2022

Preprint

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Mosquitoes are infectious vectors of a wide range of pathogens and parasites thereby transmitting several diseases like Malaria, Dengue, Chikungunya, Zika, and Japanese Encephalitis posing a major public health concern. Mostly synthetic insecticides are usually applied as a primary control strategy to manage vector-borne diseases. However excessive and non-judicious usage of such chemically derived insecticides has led to serious environmental and health issues owing to their biomagnification ability and increased toxicity towards non-target organisms. In this context, many such bioactive compounds originating from entomopathogenic microbes may serve as an alternative and environmentally benign tool for vector control. In the present paper, the entomopathogenic fungus Lecanicillium lecanii was processed to make the granules. Developed 4% LL granules have been characterized using the technique of Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). The developed formulation was also subjected to an accelerated temperature study at 40° C and was found to be stable for 3 months. Further, GCMS of the L. lecanii was also performed to screen the potential biomolecules present. The developed formulation was found to be lethal against Anopheles culicifacies with an LC50 value of 11.836 µg/mL. The findings from SEM and histopathology also substantiated the mortality effects. Further SEM EDX studies revealed that treated mosquitoes have lower nitrogen content which is correlated to a lower level of chitin whereas the control one has higher chitin content and healthy membranes. The developed LL granule formulation exhibited high toxicity against Anopheles mosquitoes due to its selective toxicity, thereby minimizing the toxicity toward non-target organisms. The granule formulations can be used as an effective biocontrol strategy against malaria-causing mosquitoes.

show abstract

Biological and chemical diversity of cytotoxin-producing symbiotic marine fungi in intertidal zone of Dalian

Cited by 8 publications

References 37 publications

Larvicidal, Histopathological, Antibacterial Activity of Indigenous Fungus Penicillium sp. Against Aedes aegypti L and Culex quinquefasciatus (Say) (Diptera: Culicidae) and Its Acetylcholinesterase Inhibition and Toxicity Assessment of Zebrafish (Danio rerio)

Larvicidal, Histopathological, Antibacterial Activity of Indigenous Fungus Penicillium sp. Against Aedes aegypti L and Culex quinquefasciatus (Say) (Diptera: Culicidae) and Its Acetylcholinesterase Inhibition and Toxicity Assessment of Zebrafish (Danio rerio)

Novel development of Lecanicillium lecanii-based granules as a platform against malarial vector Anopheles culicifacies

Novel development of Lecanicillium lecanii-based granules as a platform against malarial vector Anopheles culicifacies

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