Biological and ecological investigations on thiamineless dwarf-colony variants of Staphylococcus aureus of bovine udder origin

Ziv, G.; Sompolinsky, D

doi:10.1016/s0034-5288(18)33424-6

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…It is possible that S. aureus having the SCV phenotype contribute to chronic mastitis. SCVs have been isolated from cases of bovine mastitis [22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Identification of antimicrobial compounds active against intracellularStaphylococcus aureus

Malouin

Brouillette

Martínez

et al. 2005

FEMS Immunology &Amp; Medical Microbiology

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Small-colony variants (SCVs) of Staphylococcus aureus exhibit characteristics of bacteria that can penetrate mammalian cells and remain intracellular and innocuous for indefinite periods. These properties make SCVs a convenient tool that can be used to identify new antibacterial agents having activity against intracellular, quiescent bacteria. Agents active against SCVs could be useful in the treatment of chronic staphylococcal infections such as bovine mastitis. An hemB deletion mutant of S. aureus Newbould, a bovine mastitis isolate, having a stable, genetically defined SCV phenotype, was used in a screening program to identify compounds active against intracellular, gram-positive bacteria. Out of more than 260,000 compounds screened, nine compounds having the desired properties were identified. The range of MICs against gram-positive bacteria was < or = 0.12-32 microg ml-1. One of the compounds (no. 8) showed excellent activity against gram-positive (MICs < or = 0.12 microg ml-1) and gram-negative (MICs < or = 0.12-4 microg ml-1) bacteria. Each of the nine compounds demonstrated efficacy in a neutropenic mouse thigh infection model. Two compounds, including compound no. 8, reduced numbers of bacteria in a mouse mastitis model of infection. Application of a stepwise screening process has identified lead compounds that may be useful for treating persistent, intracellular infections.

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“…It is possible that S. aureus having the SCV phenotype contribute to chronic mastitis. SCVs have been isolated from cases of bovine mastitis [22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Identification of antimicrobial compounds active against intracellularStaphylococcus aureus

Malouin

Brouillette

Martínez

et al. 2005

FEMS Immunology &Amp; Medical Microbiology

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Chronisch rezidivierende Infektionen der Haut und Weichgewebe durch Staphylococcus aureus

et al. 2014

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Staphylococcus aureus is one of the major pathogens causing chronic skin and soft tissue infections. Particularly isolates producing Panton-Valentine leukocidin (PVL) comprising methicillin-susceptible and community-associated methicillin-resistant S. aureus (CA-MRSA) have been associated with more aggressive and persistent or relapsing courses. Beyond classical resistance mechanisms, functional resistance as shown by the small colony-variant (SCV) phenotype could be also responsible for treatment failures, despite the administration of antibiotics tested in vitro as susceptible. Also this phenotype has been associated with chronic courses of infections often with multiple exacerbations. Due to their ability to persist intracellularly, SCVs are protected from host defense and antibiotic treatment if only extracellularly active agents are administered. Reduced growth, abnormal colony morphology and changes in the metabolism of the SCVs aggravate drastically their identification, differentiation and susceptibility testing. The diagnostic and therapeutic challenges of PVL-positive and SCV isolates necessitate close collaboration with microbiological and infectious disease specialists.

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Studies on the pathogenicity of thiamineless dwarf-colony variants of Staphylococcus aureus isolated from the bovine udder

Ziv

Sompolinsky

1976

Research in Veterinary Science

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Biological and ecological investigations on thiamineless dwarf-colony variants of Staphylococcus aureus of bovine udder origin

Cited by 3 publications

References 19 publications

Identification of antimicrobial compounds active against intracellularStaphylococcus aureus

Identification of antimicrobial compounds active against intracellularStaphylococcus aureus

Chronisch rezidivierende Infektionen der Haut und Weichgewebe durch Staphylococcus aureus

Studies on the pathogenicity of thiamineless dwarf-colony variants of Staphylococcus aureus isolated from the bovine udder

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