Biological and Genomic Characteristics of MaMV-DH01, a Novel Freshwater
            <i>Myoviridae</i>
            Cyanophage Strain

Meng, Lihui; Fei, Ke; Zhang, Qi-Ya; Zhao, Zhe

doi:10.1128/spectrum.02888-22

Cited by 2 publications

(2 citation statements)

References 58 publications

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“…The disease is characterized by high fever, superficial lymphadenopathy, anemia, anorexia, lethargy, respiratory distress, and death, and Giemsa-stained blood smears showing polymorphic erythrocytes parasitized by Theileria [14,15]. In recent years, Theileria luwenshuni, Theileria uilenbergi, and Theileria ovis have been reported to infect sheep in China [16,17,18]. Anaplasma belongs to Rickettsiales Anaplasmataceae, which is an obligate and intracellular parasitic Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological and Molecular Characteristics of Piroplasmids and Anaplasma spp. in Tan Sheep, Ningxia, Northwest China

Zhou,

Li,

Zhou

et al. 2024

Transboundary and Emerging Diseases

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Piroplasmosis and anaplasmosis are important zoonotic diseases of animal origin, which can be transmitted by ticks to infect animals. However, there is limited information on the infection of piroplasmosis and anaplasmosis in Tan sheep in Ningxia, China. In order to understand the prevalence of piroplasmosis and anaplasmosis in Tan sheep in Ningxia, 150 blood samples of Tan sheep from farms in five urban areas of Ningxia were detected by PCR, and some positive samples were sequenced to establish a phylogenetic tree. PCR revealed that the prevalence of Anaplasma spp. in Tan sheep in Ningxia was 28.0%. The overall prevalence of Piroplasmids was 33.3%, of which Theileria spp. and Babesia spp. were 20.7% and 12.7%, respectively. Among the samples of different ages, the highest detection rates of Piroplasmids and Anaplasma spp. were found in Tan sheep aged 20–30 months, and the detection rate of Theileria spp., Babesia spp., and Anaplasma spp. were 25.4%, 23.6%, and 36.3%, respectively. In this study, one Theileria species was identified as Theileria uilenbergi, two Babesia species were identified as Babesia molasi and Babesia ovis, and two Anaplasma species were identified as Anaplasma ovis and Anaplasma phagocytophilum, and the dominant species were A. ovis and T. uilenbergi. To the best of our knowledge, this is the first report detailing the infection rate and genotype of Piroplasmids and Anaplasma spp. in Tan sheep in Ningxia, China. The results of this study provide valuable data for understanding the epidemiology of tick-borne disease in Tan sheep in Ningxia, China, and lay a theoretical foundation for the prevention and control of piroplasmosis and anaplasmosis in Tan sheep in Ningxia, northwest China.

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Section: Introductionmentioning

confidence: 99%

Epidemiological and Molecular Characteristics of Piroplasmids and Anaplasma spp. in Tan Sheep, Ningxia, Northwest China

Zhou,

Li,

Zhou

et al. 2024

Transboundary and Emerging Diseases

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“…Although research on freshwater viruses is relatively limited compared to their marine counterparts, various cases of the presence of photosynthesis-related genes in the genomes of freshwater cyanophages have already been reported (Gao et al, 2012;Meng et al, 2023b;Nadel et al, 2019;Ou et al, 2015;Yoshida et al, 2008). The strain-specific double-stranded DNA lytic myovirus Ma-LMM01 infecting the toxic, bloom-forming Microcystis aeruginosa NIES-298 is arguably the most comprehensively characterized freshwater cyanophage to date (e.g., Morimoto et al, 2018;Yoshida et al, 2008;Yoshida et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Structural models predict a significantly higher binding affinity between the NblA protein of cyanophage Ma-LMM01 and the phycocyanin ofMicrocystis aeruginosaNIES-298 compared to the host homolog

Meza-Padilla,

McConkey,

Nissimov

2024

Preprint

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Horizontal gene transfer events between viruses and hosts are widespread across the virosphere. In cyanophage-host systems, such events often involve the transfer of genes involved in photosynthetic processes. The genome of the lytic cyanomyovirus Ma-LMM01 infecting the toxic, bloom-forming, freshwaterMicrocystis aeruginosaNIES-298 contains a homolog of thenon-bleaching A(nblA) gene, which was probably acquired from its host. The function of the NblA protein is to disassemble phycobilisomes, cyanobacterial light harvesting complexes that can comprise up to half of the cellular soluble protein content. NblA thus plays an essential dual role in cyanobacteria: it protects the cell from high light intensities and increases the intracellular nitrogen pool under nutrient limitation. NblA has previously been shown to interact with phycocyanin, one of the main components of phycobilisomes. Using structural modeling and protein-protein docking, we show that the NblA dimer of Ma-LMM01 is predicted to have a significantly higher binding affinity forM. aeruginosaNIES-298 phycocyanin (αβ)6hexamers, compared to the host homolog. Protein-protein docking suggests that the viral NblA structural model is able to bind deeper into the phycocyanin groove. The main structural difference between the virus and host NblA appears to be an additional α-helix near the N-terminus of the viral NblA, which could be partly responsible for the deeper binding into phycocyanin. This unique helical region, absent in the cellular NblA, would be expected to constitute a viral evolutionary innovation. We propose that a higher binding affinity of NblA to the host phycocyanin may represent a selective advantage for the virus, whose rapid infection cycle requires an increased phycobilisome degradation rate that is not fulfilled by the NblA of the host.

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Biological and Genomic Characteristics of MaMV-DH01, a Novel Freshwater Myoviridae Cyanophage Strain

Cited by 2 publications

References 58 publications

Epidemiological and Molecular Characteristics of Piroplasmids and Anaplasma spp. in Tan Sheep, Ningxia, Northwest China

Epidemiological and Molecular Characteristics of Piroplasmids and Anaplasma spp. in Tan Sheep, Ningxia, Northwest China

Structural models predict a significantly higher binding affinity between the NblA protein of cyanophage Ma-LMM01 and the phycocyanin ofMicrocystis aeruginosaNIES-298 compared to the host homolog

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