Biological and immunological roles of proteins in the sperm of domestic animals (review)

Matoušek, J.

doi:10.1016/0378-4320(85)90071-5

Cited by 12 publications

(7 citation statements)

References 147 publications

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“…Their significance in both male and female reproduction seems to be indisputable. It is necessary to isolate and chemically determine these proteins and to study their biological functions (Matousek 1985).…”

Section: Discussionmentioning

confidence: 99%

Isolation of Ovulation-inducing Factors in the Seminal Plasma of Bactrian Camel (Camelus bactrianus) by DEAE-cellulose Chromatography

Zhao

Chen

2001

Reprod Domest Anim

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Ovulation in the Bactrian camel (Camelus bactrianus) depends upon the ovulation-inducing factor in the seminal plasma; however, little research has been conducted to isolate and identify the factor. The current study attempts to isolate and identify the bioactive fractions from the seminal plasma of Bactrian camel. The seminal plasma was fractionated by diethylamino-ethylcellulose (DEAE)-cellulose chromatography and five protein fractions were obtained. The bioactive of each fraction was estimated by rat pituitary tissue culture in vitro and by the intramuscular injection of the bioactive fraction to the female camels in vivo. The concentrations of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in the pituitary culture media before and 6 h after the addition of each fraction and in the peripheral blood plasma collected from the camel immediately before and hourly after the injection of the active fraction were measured by radioimmunoassay. The results demonstrated that the third fraction (L3) had the bioactive potential to stimulate the release of LH in vitro from 11.82 +/- 1.77 to 25.63 +/- 3.84 mIU/ml after the addition of L3 to the culture media. The in vivo concentrations of LH in the blood plasma of the camel increased from 6.43 +/- 0.14 before to 15.50 +/- 2.64 ng/ml 6 h after injection of L3. However, the concentrations of FSH did not show any significant changes either in vitro or in vivo. The results clearly demonstrated the existence of LH-releasing associated fractions in the seminal plasma that appears to be separated by DEAE-cellulose matrix and the isolated L3 fraction might be the ovulation-inducing factor or one of its components.

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Section: Discussionmentioning

confidence: 99%

Isolation of Ovulation-inducing Factors in the Seminal Plasma of Bactrian Camel (Camelus bactrianus) by DEAE-cellulose Chromatography

Zhao

Chen

2001

Reprod Domest Anim

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“…Additionally, SP plays an immunoregulatory role that is beneficial to survival of spermatozoa in the female reproductive tract. Several factors in SP, including proteins, cytokines, sex hormones, and prostaglandins, accompany the migration of spermatozoa to the female reproductive tract (Matoušek, 1985) and possess potential biological capabilities to protect spermatozoa from different pathogens, both in the male and female reproductive tract (Maegawa et al, 2002). Potential components of SP and their specific functions are briefly discussed in the upcoming text ( Figure 2).…”

mentioning

confidence: 99%

Seminal Plasma: An Essential Attribute to Spermatozoa

Juyena¹,

Stelletta²

2012

Journal of Andrology

224

178

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The biochemical composition of seminal plasma (SP) is very complex and variable among species. Advances in reproductive technologies reveal SP as a nutritive-protective medium for sperm cells suspended in it, and some SP components are very important for sperm metabolism, as well as sperm function, survival, and transport in the female reproductive tract. Biochemistry of SP is a relatively modern but rapidly expanding field of research, particularly concerning the biological significance of the various biochemical constituents of SP. However, identification of active factors in SP and mechanisms by which they act are not sufficiently understood in different species. Especially in camelids, the origin, composition, and function of the viscous component of SP remain a mystery, and only some biochemical and morphological characteristics of SP have been described. In this review, we focus on the current understanding of physiological and biochemical properties of SP in ruminants and camelids.

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“…The immunosuppressive effects of seminal plasma factors are well known (Matousek, 1985;Alexander & Anderson, 1987), and may aid sperm survival in the female genital tract. While having a possible advantage to sperm survival, seminal plasma factors may also impair microflora control.…”

Section: Introductionmentioning

confidence: 99%

Effect of bovine seminal plasma on neutrophil phagocytosis of bull spermatozoa

Strzemienski¹

1989

Reproduction

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Seminal plasma was obtained from bulls of known fertility and was assessed for its effect on serum-induced phagocytosis of bull spermatozoa. A non-dialysable component was found to inhibit neutrophil phagocytic uptake of spermatozoa. The component was not destroyed by heating (56 degrees C for 30 min) or removed by ether. Use of a bactericidal assay confirmed the inhibition and suggested that inhibition does not permanently impair neutrophil function. Immunoperoxidase staining demonstrated the presence of bovine IgM, IgG1 and IgG2 on spermatozoa incubated in serum. Affinity of spermatozoa for the immunoglobulins was reduced when seminal plasma was added to the serum. These results suggest that bull seminal plasma can regulate phagocytic ingestion of spermatozoa. While the mechanism of this regulation remains obscure, it may be important in providing protection to spermatozoa immediately after ejaculation.

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Biological and immunological roles of proteins in the sperm of domestic animals (review)

Cited by 12 publications

References 147 publications

Isolation of Ovulation-inducing Factors in the Seminal Plasma of Bactrian Camel (Camelus bactrianus) by DEAE-cellulose Chromatography

Isolation of Ovulation-inducing Factors in the Seminal Plasma of Bactrian Camel (Camelus bactrianus) by DEAE-cellulose Chromatography

Seminal Plasma: An Essential Attribute to Spermatozoa

Effect of bovine seminal plasma on neutrophil phagocytosis of bull spermatozoa

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