Biological and molecular characterization of chicken anaemia virus isolates of Indian origin

Natesan, Senthilkumar; Kataria, J.M.; Dhama, Kuldeep; Rahul, S.; Baradhwaj, N.

doi:10.1016/j.virusres.2005.11.017

Cited by 65 publications

(55 citation statements)

References 22 publications

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“…Normally, the virus does not grow in any of the commonly used primary cells and cell lines. Marek's disease virus or avian leucosis virus transformed lymphoblastoid cell lines are susceptible to this virus and the virus usually multiplies with low titre (Natesan et al, 2006). For this reason, different molecular diagnostic methods have been developed for CAV diagnosis, out of which polymerase chain reaction (PCR) seems to be the fastest and most sensitive method for the detection of virus nucleic acid ( Jeurissen et al, 1992).…”

Section: Advances In Animal and Veterinary Sciences May 2016 | Volumementioning

confidence: 99%

Molecular and Pathological Analysis of Chicken Anemia Virus Isolated from Field Infection in Three Egyptian Provinces

Hussein¹,

Arafa²,

Anwar³

et al. 2016

Adv. Anim. Vet. Sci.

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Section: Advances In Animal and Veterinary Sciences May 2016 | Volumementioning

confidence: 99%

Molecular and Pathological Analysis of Chicken Anemia Virus Isolated from Field Infection in Three Egyptian Provinces

Hussein¹,

Arafa²,

Anwar³

et al. 2016

Adv. Anim. Vet. Sci.

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“…The disease has been reported from poultry flocks of some states of the country, and included in the list of emerging and important viruses that are a severe threat to the Indian poultry industry (Venugopalan et al, 1994;Kataria et al, 1999;Verma et al, 2005;Natesan et al, 2006;Praveen et al, 2008;Bhatt et al, 2011;Wani et al, 2013). Although a few sequences of CAV from India are available in GenBank (NCBI), no systematic analysis of Indian CAV strains has been performed to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Chicken Infectious Anemia Virus Isolated From Commercial Poultry with Respiratory Disease Complex in India

Gowthaman¹

2014

Adv. Anim. Vet. Sci.

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“…The viral genome consists of a circular, non-enveloped, negative-sense, single stranded DNA of 2.3 kb that contains three partially overlapping major open reading frames (ORFs) encoding three proteins; namely, VP1, VP2 and VP3 (Todd 2000;Miller et al 2005;Natesan et al 2006a). The VP1 (51 kD) acts as a major capsid protein, VP2 (24 kD) acts as a scaffold protein and is essential for the virus assembly, while VP3 (13 kD), also known as apoptin, is important for the disease pathogenesis and apoptosis (Noteborn 2004;Miller et al 2005;Natesan et al 2006b).…”

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confidence: 99%

“…Chicks (n = 32), at the age of six weeks were randomly and equally divided into two groups, Infected and Control. All the chicks of the Infected group were inoculated intramuscularly with a 10 4.5 median tissue culture infective dose (TCID 50 ) of the MDCC-MSB1 passaged CIAV-E isolate, while the control chicks received uninfected cell lysate as described previously (Natesan et al 2006a). All the chicks were regularly monitored for the development of clinical signs of the disease and weighed at five day intervals.…”

mentioning

confidence: 99%

Correlation between cytokine profile,antibody titre and viral load during sub-clinical chicken anaemia virus infection

Wani¹,

Dhama²,

Latheef³

et al. 2014

Vet. Med. - Czech

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Th e present work was carried out to investigate the correlation between viral load and the cytokine profi le and virus specifi c antibody levels at fi ve day intervals up to 25 days post infection (dpi) in various tissues of chicks infected with chicken infectious anaemia virus (CIAV) at six weeks of age (sub-clinical infection). For determining the viral load in the various tissues, recombinant plasmid VP2-pET32b was prepared and serially diluted tenfold for the generation of a standard curve using real time polymerase chain reaction (PCR). Cytokine fold changes in mRNA levels of IFN-γ, IL-1, IL-2, IL-12 in the spleen and IFN-γ, IL-12 in freshly collected whole blood were determined. Th e results showed that peak viral load occurs between 10-20 days post infection in the lymphoid tissues viz., thymus, spleen, liver, bone marrow, bursa and also blood, being highest in blood followed by thymus. A varied response in the expression levels of individual cytokines was observed during all the intervals post infection. Th e IFN-γ increased two to fi ve fold in blood and spleen while IL-2 decreased in the splenic tissue during the same period. Peak IFN-γ coincided with peak viral load in the spleen at 10 dpi while in blood it peaked earlier at fi ve days post infection and remained high during the peak viral load at 10-20 dpi. Virus specifi c antibodies were signifi cantly higher at 15 dpi and were thereafter found to be strongly associated with viral load regression although higher concentrations of virus remained in the blood and thymic tissues until 25 dpi, indicating a role of other immune components in virus clearance. In conclusion, this study reports a negative correlation of viral load and the progression of the antibody response and IFN-γ cytokine expression in chicks infected with CIAV during their sub-clinical infectious stage. However, cytokine expression from the various individual immune cells like monocytes, heterophils, CD4 + T, CD8 + T cells and others during the pathogenesis of this immunosuppressive poultry pathogen remains to be elucidated.

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Biological and molecular characterization of chicken anaemia virus isolates of Indian origin

Cited by 65 publications

References 22 publications

Molecular and Pathological Analysis of Chicken Anemia Virus Isolated from Field Infection in Three Egyptian Provinces

Molecular and Pathological Analysis of Chicken Anemia Virus Isolated from Field Infection in Three Egyptian Provinces

Molecular Characterization of Chicken Infectious Anemia Virus Isolated From Commercial Poultry with Respiratory Disease Complex in India

Correlation between cytokine profile,antibody titre and viral load during sub-clinical chicken anaemia virus infection

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