Biological and molecular characterization of a begomovirus associated with yellow mosaic vein mosaic disease of pumpkin from Northern India

Abstract

The biological and molecular properties of Squash leaf curl China virus from Varanasi, India (SLCCNV-IN[IN:Var:Pum]) were characterized. SLCCNV-IN[IN:Var:Pum] could be transmitted by grafting and through whitefly transmission. The complete DNA-A and DNA-B components were amplified through PCR using specific DNA-A and DNA-B primers. The DNA-A of the isolate was comprised of 2,738 nucleotides, encoding typical six open reading frames, and DNA-B of 2,704 nucleotides, encoding two ORFs. Genome organization of the isolate was typical of an old world bipartite begomovirus. Comparisons showed that DNA-A and its intergenic region have the highest sequence identity (97.6 and 97.4%, respectively) with the SLCCNV-IN[IN:Luc:Pum]; (DQ026296). This data suggested that the isolate is a same begomovirus species for which the name Squash leaf curl China virus-India[India:Varanasi:Pumpkin] is proposed. DNA-B showed maximum sequence identity (89.2%) with SLCCNV-IN[IN:Coi:Pum] (AY184488). Phylogenetic analysis of the present isolate showed close relationship to other cucurbit-infecting geminiviruses. This is the first evidence of occurrence of the bipartite Squash leaf curl China virus associated with severe yellow mosaic disease of pumpkin in northern India.

Phytochemicals and antioxidative enzymes defence mechanism on occurrence of yellow vein mosaic disease of pumpkin (Cucurbita moschata)

“…Characterization of pumpkin-infecting begomoviruses from India indicates the existence of at least two major viruses namely, SLCCNV in the South India and ToLCNDV in North India (Singh et al 2009; Maruthi et al 2007) associated with yellow vein mosaic disease of pumpkins. In the present study, another new strain of begomoviruses namely Tomato leaf curl Palampur virus (ToLCPaV) associated with yellow vein mosaic disease of pumpkin was identified.…”

A Novel Strain of Tomato Leaf Curl New Delhi Virus Has Spread to the Mediterranean Basin

“…and cucumber ( Cucumis sativus L.) [10,11,12,13,14]. In tomato, the virus causes severe stunting of plants, which exhibit upward and downward rolling and crinkling of the leaves, interveinal yellowing and vein clearing, with infected plants presenting partial or complete yield loss depending on the growth stage at which infection occurs [15].…”

A Major QTL Located in Chromosome 8 of Cucurbita moschata Is Responsible for Resistance to Tomato Leaf Curl New Delhi Virus

“…], cucumber (Cucumis sativus L.), watermelon (Citrullus lanatus L), melon (C. melo L.), and different types of squashes (Cucurbita spp.) (Sohrab et al, 2003;Ito et al, 2008;Singh et al, 2009;Chang et al, 2010;Roy et al, 2013). Recently, it has been reported to be affecting species of other plant families, such as opium poppy (Papaver somniferum L., Papaveraceae) (Srivastava et al, 2016), cotton (Gossypium hirsutum L., Malvaceae) (Zaidi et al, 2016), soybean (Glycine max, Fabaceae L.…”