Biological and molecular characterization of a putative new sobemovirus infecting Imperata cylindrica and maize in Africa

Sérémé, Drissa; Lacombe, Séverine; Konaté, Moumouni; Pinel‐Galzi, Agnès; Traoré, Valentin Stanislas Edgar; Hébrard, Eugénie; Traoré, Oumar; Brugidou, Christophe; Fargette, Denis; Konaté, G.

doi:10.1007/s00705-008-0190-y

Cited by 22 publications

(11 citation statements)

References 21 publications

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“…Up to now, 11 sobemovirus genomes have been fully sequenced. All sequenced sobemoviruses exhibit common genomic organization: the RdRp is encoded by ORF2b by the −1 PRF mechanism (McGavin and Macfarlane, 2009;Meier and Truve, 2007;Sérémé et al, 2008). The analysis of their cis-acting −1 PRF signals indicate the presence of an identical UUUAAAC slippery sequence, a seven nucleotide spacer region 1 (exception is Ryegrass mottle virus where the spacer is 8 nucleotides long), and a putative stem-loop secondary structure (Fig.…”

Section: Discussionmentioning

confidence: 99%

Stem-loop structure of Cocksfoot mottle virus RNA is indispensable for programmed −1 ribosomal frameshifting

et al. 2009

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The -1 programmed ribosomal frameshifting (-1 PRF) mechanism utilized by many viruses is dependent on a heptanucleotide slippery sequence and a downstream secondary structure element. In the current study, the RNA structure downstream from the slippery site of cocksfoot mottle sobemovirus (CfMV) was proven to be a 12bp stem-loop with a single bulge and a tetranucleotide loop. Several deletion and insertion mutants with altered stem-loop structures were tested in wheat germ extract (WGE) for frameshifting efficiency. The impact of the same mutations on virus infectivity was tested in oat plants. Mutations shortening or destabilizing the stem region reduced significantly but did not abolish -1 PRF in WGE. The same mutations proved to be deleterious for virus infection. However, extending the loop region to seven nucleotides had no significant effect on frameshifting efficiency in WGE and did not hamper virus replication in infected leaves. This is the first report about the experimentally proven RNA secondary structure directing -1 PRF of sobemoviruses.

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Section: Discussionmentioning

confidence: 99%

Stem-loop structure of Cocksfoot mottle virus RNA is indispensable for programmed −1 ribosomal frameshifting

et al. 2009

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“…The symptoms observed on Imperata cylindrical were more similar to those of rice yellow mottle disease. The non-detection of RYMV in this species suggested that these symptoms may be probably due to Imperata yellow mottle virus, a Sobemovirus closely related to RYMV [19] which were found in West Africa [20].…”

Section: Discussionmentioning

confidence: 86%

Detection and Serological Characterization of Rice Yellow Mottle Virus in Central African Republic

Longué¹,

Zinga²,

Semballa³

et al. 2016

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Serological and biological detection of Rice Yellow Mottle Virus (RYMV) in leaf samples belonging from cultivated rice species and wild grasses revealed 201 positive detections. All these isolates occurred exclusively on cultivated rice species (Oryza sativa L. and O. glaberrima Steud). A relationship was found between cultural practices and presence of RYMV in fields (χ² = 108.83, df = 1, P < 0.001). The serological characterization using Monoclonal antibodies (Mabs A and D), showed homogenous reaction with Mabs A alone. These results indicated that Ser1 serotype is present in the south of Central African Republic.

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“…SMAMV clustered with RYMV and IYMV but the bootstrap support was low due to the short sequences used and high divergence between viruses. However, it is noteworthy that SMAMV clustered with RYMV and IYMV, two sobemoviruses also reported only from Africa (8).…”

Section: Resultsmentioning

confidence: 94%

Snake melon asteroid mosaic virus, a Tentative New Member of the GenusSobemovirusInfecting Cucurbits

Lecoq¹,

Dafalla

Delécolle

et al. 2011

Plant Disease

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A virus isolate (Su-95-67) was obtained from a snake melon (Cucumis melo var. flexuosus) plant presenting severe chlorotic spots, mosaic, stunting, and leaf deformations collected in Eastern Sudan in 1995. Su-95-67 was easily mechanically transmissible and had a host range limited to a few cucurbit species. Isometric virus particles approximately 30 nm in diameter were observed in leaf dip preparations. A cytopathological study did not reveal alterations specific for a virus genus or family. A polyclonal antiserum was obtained and used in double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Su-95-67 was transmitted by seed at a low rate, by the red melon beetle (Aulacophora foveicollis), but not by the melon aphid (Aphis gossypii). Because Su-95-67 shared several properties with sobemoviruses, generic Sobemovirus reverse-transcription polymerase chain reaction primers were developed. They allowed amplification of a 384-bp fragment from extracts of plants infected by two sobemoviruses or by Su-95-67 but not from healthy plants extracts. Sequence comparison confirmed that Su-95-67 belongs to a new tentative Sobemovirus species for which we propose the name Snake melon asteroid mosaic virus (SMAMV). DAS-ELISA tests conducted on extracts of virus-infected cucurbit plants collected from 1992 to 2003 revealed the presence of SMAMV in 10.2% of 600 samples originating from different regions of Sudan.

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Biological and molecular characterization of a putative new sobemovirus infecting Imperata cylindrica and maize in Africa

Cited by 22 publications

References 21 publications

Stem-loop structure of Cocksfoot mottle virus RNA is indispensable for programmed −1 ribosomal frameshifting

Stem-loop structure of Cocksfoot mottle virus RNA is indispensable for programmed −1 ribosomal frameshifting

Detection and Serological Characterization of Rice Yellow Mottle Virus in Central African Republic

Snake melon asteroid mosaic virus, a Tentative New Member of the GenusSobemovirusInfecting Cucurbits

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