Biological and molecular studies of a cypovirus from the black fly Simulium ubiquitum (Diptera: Simuliidae)

Green, Terry B.; White, Susan; Rao, S. Venkat; Mertens, Peter P. C.; Adler, Peter H.; Becnel, James J.

doi:10.1016/j.jip.2006.10.006

Cited by 27 publications

(11 citation statements)

References 18 publications

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“…To recover the purified virus, the second instar larvae of H. armigera were infected with a suspension of NPV by spraying and the larvae were allowed to feed on artificial diet (Wakil et al, 2011) for 7 d. Then the midguts of the infected cadavers were homogenized in deionized water, filtered through muslin cloth and centrifuged at 16 000 rpm for 45 min (Shapiro et al, 2005;Green et al, 2006). The purified virus was given washes three times in distilled water, and held in 0.1 mM NaOH at 5 °C.…”

Section: Nuclear Polyhedrosis Virus (Npv)mentioning

confidence: 99%

Insecticidal Efficacy of Azadirachta indica, Nucleopolyhedrovirus and Chlorantraniliprole Singly or Combined against Field Populations fo Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Wakil

Ghazanfar

Nasir

et al. 2012

Chilean J. Agric. Res.

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The development of resistance in cosmopolitan insect Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) forced the researchers for alternative control measures. In the present study, insecticidal efficacy of formulations of Azadirachta indica, a Nucleopolyhedrovirus (NPV), and new anthranilic diamide insecticide (chlorantraniliprole) formulations was determined against 2 nd , through 5 th larval instars of H. armigera collected from diverse geographical locations in the Punjab province, Pakistan. Azadirachta indica was applied at 5 µL L -1 ; NPV at 2.1 × 10 5 polyhedral occlusion bodies (POB) mL -1 and chlorantraniliprole at 0.01 µL L -1 , either alone or in combinations with each other. The bioassays were conducted at 27 ± 1 °C and 65 ± 5% relative humidity. The mortality varied greatly among treatments, larval instars, and locations. The combinations of NPV with A. indica and chlorantraniliprole caused higher mortality, pupation and produced an additive effect compared to their application singly in all the tested populations. The population from Rawalpindi was always susceptible while the Gujranwala was the resistant. The results herein suggest that the effectiveness of NPV and A. indica can be improved by the presence of chlorantraniliprole against the larvae of H. armigera.

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Section: Nuclear Polyhedrosis Virus (Npv)mentioning

confidence: 99%

Insecticidal Efficacy of Azadirachta indica, Nucleopolyhedrovirus and Chlorantraniliprole Singly or Combined against Field Populations fo Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Wakil

Ghazanfar

Nasir

et al. 2012

Chilean J. Agric. Res.

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“…At 7th day after infection, midguts were homogenized in deionized water and strained through 35-lm-pore-size mesh nylon cloth to remove large debris. The filtrate was layered on top of HS-40 Ludox continues gradient and centrifuged at 16,000g for 45 min (Green et al, 2006;Shapiro et al, 2005). The resulting band containing purified virus was recovered, washed in sterile distilled water three times, and held in 0.1 mM NaOH at 5°C.…”

Section: Cytoplasmic Polyhedrosis Virus Preparationmentioning

confidence: 99%

“…The genomic dsRNA of HaCPV was extracted form purified polyhedra, using QIAamp Viral Mini Kit from Qiagen (Green et al, 2006;Shapiro et al, 2005;Hagiwara et al, 2002). After precipitating, washing, drying, and resuspending the RNA in RNase free water, the genomic RNA was analyzed by 1% agarose gel electrophoresis (AGE).…”

Section: Electropherotype Analysis Of Hacpvmentioning

confidence: 99%

Effects of Bacillus thuringiensis toxin Cry1Ac and cytoplasmic polyhedrosis virus of Helicoverpa armigera (Hübner) (HaCPV) on cotton bollworm (Lepidoptera: Noctuidae)

Marzban

Liu

et al. 2009

Journal of Invertebrate Pathology

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“…It also shares 27%–32% amino acid identity with the polyhedrin proteins of Simulium ubiquitum cypovirus 20 [5] and CoCPV-16 (GenBank accession number: ABW87644). Because the polyhedrin proteins of all CPVs are highly conserved, a neighbor-joining phylogenetic tree was constructed with the amino acid sequences of the polyhedrin proteins of 19 CPV species (Figure 4).…”

Section: Resultsmentioning

confidence: 99%

“…The CPVs have so far been classified into 21 types based on the electrophoretic migration patterns of their dsRNA genome segments [5]. Several CPVs have been completely or partially sequenced, including Bombyx mori cypovirus 1 (BmCPV-1) [6], [7], [8], [9], [10], [11], Dendrolimus punctatus cypovirus 1 (DpCPV-1) [12], [13], and Antheraea mylitta cypovirus 4 (AmCPV-4) [14], [15], [16], [17], [18], [19], [20].…”

Section: Introductionmentioning

confidence: 99%

Genomic and Biological Characterization of a New Cypovirus Isolated from Dendrolimus punctatus

et al. 2014

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A novel cypovirus (designated DpCPV-MC) was isolated from the pine moth Dendrolimus punctatus using serial in vivo cloning procedures. DpCPV-MC occurs in typical polyhedral occlusion bodies, containing a number of spherical virions. Laboratory bioassays indicated that the infectivity of DpCPV-MC against second-instar Spodoptera exigua larvae does not differ significantly from that of Dendrolimus punctatus cypovirus 1. Full-length amplification of the DpCPV-MC cDNAs identified 16 dsRNA genome segments. Each segment encodes one open reading frame with unique conserved terminal sequences at the 5′ and 3′ ends, which differ from those of all previously reported cypoviruses. On a phylogenetic tree based on the amino acid sequences of the polyhedrin of 19 cypovirus species, DpCPV-MC was closest to the type-4 cypoviruses. Homology searches showed that ten segments of DpCPV-MC (S1, S2, S3, S4, S5, S7, S8, S9, S12, and S13) encode putative CPV structural and nonstructural proteins, three segments (S6, S10 and S14) encode putative insect proteins or other viral proteins, and the other three segments (S11, S15, and S16) encode proteins that have no obvious sequence similarity to any known protein. Based on RNA secondary structures analysis, two segments of them (S11 and S16) were predicted to possibly transcript less efficiently than the other segments. We speculate that DpCPV-MC is composed of several genotypes. The ten CPV-related segments constantly exist in all genotypes, and one or two of the six CPV-unrelated segments co-exist with the ten CPV-related segments in one DpCPV-MC genotype, thus each virion contains no more than 12 segments. Based on our results and the literature, DpCPV-MC is a new cypovirus (Cypovirus 22, strain DpCPV-22).

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Biological and molecular studies of a cypovirus from the black fly Simulium ubiquitum (Diptera: Simuliidae)

Cited by 27 publications

References 18 publications

Insecticidal Efficacy of Azadirachta indica, Nucleopolyhedrovirus and Chlorantraniliprole Singly or Combined against Field Populations fo Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Insecticidal Efficacy of Azadirachta indica, Nucleopolyhedrovirus and Chlorantraniliprole Singly or Combined against Field Populations fo Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Effects of Bacillus thuringiensis toxin Cry1Ac and cytoplasmic polyhedrosis virus of Helicoverpa armigera (Hübner) (HaCPV) on cotton bollworm (Lepidoptera: Noctuidae)

Genomic and Biological Characterization of a New Cypovirus Isolated from Dendrolimus punctatus

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