Biological and Rheological Properties of Collagen Cross-Linked with Glutaraldehyde

Nashchekina, Yu. A.; Lukonina, O. A.; Darvish, Diana; Нащекин, А. В.; Elokhovskii, V. Yu.; Yudin, V. E.; Mikhailova, Natalia

doi:10.1134/s1063784220090224

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…As mentioned above, the first band has been related to N-H stretching vibration from amine functionalities (-NH 2 ) existing in CLG structure of BP whereas the last band is due to asymmetric and symmetric C-H stretching vibrations of methylene groups. The decrease in intensity of the band at 3296 cm −1 after the crosslinking process is due that these groups reacts with GA to yield a Schiff base [46][47][48][49]. Similarly, the increase in the intensities of the bands of methylene groups was observed because a greater number of methylene groups belonging to the GA are presented in the GC-BP sample.…”

Section: Discussionmentioning

confidence: 90%

In vitro evaluation of crosslinked bovine pericardium as potential scaffold for the oral cavity

Ordoñez-Chavez

Rodríguez-Fuentes

Peñaloza-Cuevas

et al. 2023

BME

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BACKGROUND: Bovine pericardium (BP) is a scaffold widely used in soft tissues regeneration; however, its calcification in contact with glutaraldehyde, represent an opportunity for its application in hard tissues, such as bone in the oral cavity. OBJECTIVE: To develop and to characterize decellularized and glutaraldehyde-crosslinked bovine pericardium (GC-BP) as a potential scaffold for guided bone regeneration GBR. METHODS: BP samples from healthy animals of the bovine zebu breed were decellularized and crosslinked by digestion with detergents and glutaraldehyde respectively. The resulting cell-free scaffold was physical, chemical, mechanical, and biologically characterized thought hematoxylin and eosin staining, DNA quantification, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), uniaxial tensile test, cell viability and live and dead assay in cultures of dental pulp stem cells (DPSCs). RESULTS: The decellularization and crosslinking of BP appeared to induce conformational changes of the CLG molecules, which led to lower mechanical properties at the GC-BP scaffold, at the same time that promoted cell adhesion and viability of DPSCs. CONCLUSION: This study suggests that the decellularized and GC-BP is a scaffold with the potential to be used promoting DPSCs recruitment, which has a great impact on the dental area.

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Section: Discussionmentioning

confidence: 90%

In vitro evaluation of crosslinked bovine pericardium as potential scaffold for the oral cavity

Ordoñez-Chavez

Rodríguez-Fuentes

Peñaloza-Cuevas

et al. 2023

BME

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“…Glutaraldehyde is a straight-chain saturated dialdehyde with five carbons [ 28 ], whilst dialdehyde starch is a polysaccharide derived from modified starch [ 36 ]. Both Glutaraldehyde and dialdehyde starch function by two highly reactive aldehydic groups forming covalent bonds with free amine groups on adjacent collagen peptide chains [ 28 , 37 ]. Glutaraldehyde is noted to be one of the most effective crosslinkers; however, unreacted molecules result in cytotoxic responses [ 38 ] when compared to dialdehyde starch, which has good biocompatibility [ 39 ].…”

Section: Collagenmentioning

confidence: 99%

“… Collagen chemical crosslinker mechanisms glutaraldehyde, dialdehyde starch, genipin, and EDC/NHS [ 37 , 41 , 46 , 47 ]. …”

Section: Figurementioning

confidence: 99%

Collagen Alignment via Electro-Compaction for Biofabrication Applications: A Review

Carr

Chen²,

Chung³

et al. 2022

Polymers

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As the most prevalent structural protein in the extracellular matrix, collagen has been extensively investigated for biofabrication-based applications. However, its utilisation has been impeded due to a lack of sufficient mechanical toughness and the inability of the scaffold to mimic complex natural tissues. The anisotropic alignment of collagen fibres has been proven to be an effective method to enhance its overall mechanical properties and produce biomimetic scaffolds. This review introduces the complicated scenario of collagen structure, fibril arrangement, type, function, and in addition, distribution within the body for the enhancement of collagen-based scaffolds. We describe and compare existing approaches for the alignment of collagen with a sharper focus on electro-compaction. Additionally, various effective processes to further enhance electro-compacted collagen, such as crosslinking, the addition of filler materials, and post-alignment fabrication techniques, are discussed. Finally, current challenges and future directions for the electro-compaction of collagen are presented, providing guidance for the further development of collagenous scaffolds for bioengineering and nanotechnology.

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“…Glutaraldehyde is widely used crosslinking agent because it is cheap and very effective crosslinker. Glutaraldehyde, a linear 5-carbon dialdehyde, is transparent and colorless [19]. It is an oily liquid with a pungent odor that can be dissolved in water, alcohol and organic solvents in all proportions.…”

Section: Cross-linkers Used For Chitosan Cross-linkingmentioning

confidence: 99%

Modified Chitosan Forms for Cr (VI) Removal

Parlayıcı¹,

Pehlivan²

2021

Chitin and Chitosan - Physicochemical Properties and Industrial Applications [Working Title]

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The forms of utility in the wastewater treatment of chitosan-based adsorbents acquired from natural substances have attracted numerous attentions in recent years. The use of chitosan modified adsorbents for removal of the chromium has aroused great interest. When chitosan-based modified adsorbents are considered, they have got large amount of an amino (-NH2) and hydroxyl (-OH) functional groups. Such adsorbents display that they have high activity and therefore they may be extensively utilized in wastewater treatment plants to cast off chromium. In this chapter, the utility outcomes of chitosan-based substances will be explained after applying different parameters to remove Cr (VI) from the aqueous surrounding with the information obtained the use of batch adsorption systems. Application of various chitosan-based adsorbents for Cr (VI) removal application will be demonstrated in a detailed way and they will be discussed within the textual content.

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Biological and Rheological Properties of Collagen Cross-Linked with Glutaraldehyde

Cited by 10 publications

References 12 publications

In vitro evaluation of crosslinked bovine pericardium as potential scaffold for the oral cavity

In vitro evaluation of crosslinked bovine pericardium as potential scaffold for the oral cavity

Collagen Alignment via Electro-Compaction for Biofabrication Applications: A Review

Modified Chitosan Forms for Cr (VI) Removal

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