Biological and sequence analysis of a novel European isolate of Barley mild mosaic virus that overcomes the barley rym5 resistance gene

Kanyuka, K.; McGrann, Graham R. D.; Alhudaib, K.; Hariri, Djabbar; Adams, Michael J.

doi:10.1007/s00705-004-0318-7

Cited by 54 publications

(45 citation statements)

References 32 publications

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“…However, rym4/rym5 resistance-breaking isolates have recently been observed (16,17,38). rym11 is effective against all European strains of BaMMV/BaYMV (16)(17)(18). We have shown here that resistance is conferred by loss-offunction mutations in HvPDIL5-1.…”

Section: Loss-of-function Alleles Are Prevalent In Domesticated Barleymentioning

confidence: 63%

“…In European barley breeding this stimulated the extensive use of rym4/rym5, a single natural recessive resistance conferred by alleles of the EUKARYOTIC TRANSLATION INITIATION FACTOR 4E (37). However, rym4/rym5 resistance-breaking isolates have recently been observed (16,17,38). rym11 is effective against all European strains of BaMMV/BaYMV (16)(17)(18).…”

Section: Loss-of-function Alleles Are Prevalent In Domesticated Barleymentioning

confidence: 99%

“…Soil-borne transmission via the plasmodiophorid Polymyxa graminis prohibits plant protection by chemical measures, and breeding for resistant cultivars is therefore the only practicable way to prevent yield loss. The naturally occurring recessive resistance locus rym11 confers complete broadspectrum resistance to all known European isolates of BaMMV and BaYMV (16)(17)(18)(19). In the present study, we used a positional cloning strategy to identify the gene underlying rym11-based resistance.…”

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confidence: 99%

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PROTEIN DISULFIDE ISOMERASE LIKE 5-1is a susceptibility factor to plant viruses

Yang

Lüpken

Habekuß

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Protein disulfide isomerases (PDIs) catalyze the correct folding of proteins and prevent the aggregation of unfolded or partially folded precursors. Whereas suppression of members of the PDI gene family can delay replication of several human and animal viruses (e.g., HIV), their role in interactions with plant viruses is largely unknown. Here, using a positional cloning strategy we identified variants of PROTEIN DISULFIDE ISOMERASE LIKE 5-1 (HvPDIL5-1) as the cause of naturally occurring resistance to multiple strains of Bymoviruses. The role of wild-type HvPDIL5-1 in conferring susceptibility was confirmed by targeting induced local lesions in genomes for induced mutant alleles, transgene-induced complementation, and allelism tests using different natural resistance alleles. The geographical distribution of natural genetic variants of HvPDIL5-1 revealed the origin of resistance conferring alleles in domesticated barley in Eastern Asia. Higher sequence diversity was correlated with areas with increased pathogen diversity suggesting adaptive selection for bymovirus resistance. HvPDIL5-1 homologs are highly conserved across species of the plant and animal kingdoms implying that orthologs of HvPDIL5-1 or other closely related members of the PDI gene family may be potential susceptibility factors to viruses in other eukaryotic species.allele mining | resistance breeding | soil-borne virus disease | chaperone

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Section: Loss-of-function Alleles Are Prevalent In Domesticated Barleymentioning

confidence: 63%

Section: Loss-of-function Alleles Are Prevalent In Domesticated Barleymentioning

confidence: 99%

mentioning

confidence: 99%

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PROTEIN DISULFIDE ISOMERASE LIKE 5-1is a susceptibility factor to plant viruses

Yang

Lüpken

Habekuß

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…This combination of pathogens is commonly referred to as the barley yellow mosaic virus complex (BYMV). Carriers of rym4 are resistant to BaMMV, BaMMV-Sil, and BaYMV-1, but are susceptible to BaYMV-2, while those carrying rym5 are resistant to BaYMV-1, BaYMV-2, and BaMMV, but not to BaMMV-Sil (Kanyuka et al 2004). Although at least seven independent loci conferring resistance to BYMV have been identified in barley to date (Ordon et al 2005), European breeding has relied heavily on rym4 and rym5, both of which originate from single germplasm accessions.…”

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confidence: 99%

Effects of Introgression and Recombination on Haplotype Structure and Linkage Disequilibrium Surrounding a Locus EncodingBymovirusResistance in Barley

Stracke

Presterl²,

Stein

et al. 2007

Genetics

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We present a detailed analysis of linkage disequilibrium (LD) in the physical and genetic context of the barley gene Hv-eIF4E, which confers resistance to the barley yellow mosaic virus (BYMV) complex. Eightythree SNPs distributed over 132 kb of Hv-eIF4E and six additional fragments genetically mapped to its flanking region were used to derive haplotypes from 131 accessions. Three haplogroups were recognized, discriminating between the alleles rym4 and rym5, which each encode for a spectrum of resistance to BYMV. With increasing map distance, haplotypes of susceptible genotypes displayed diverse patterns driven mainly by recombination, whereas haplotype diversity within the subgroups of resistant genotypes was limited. We conclude that the breakdown of LD within 1 cM of the resistance gene was generated mainly by susceptible genotypes. Despite the LD decay, a significant association between haplotype and resistance to BYMV was detected up to a distance of 5.5 cM from the resistance gene. The LD pattern and the haplotype structure of the target chromosomal region are the result of interplay between low recombination and recent breeding history.T HE ever-increasing availability of nucleotide sequence, and the concomitant improvement that this brings to our understanding of the organization of complex crop plant genomes, has created the opportunity to identify trait-related genes and to analyze their allelic diversity. The association between phenotype and genotype in diverse populations represents a powerful approach, but the applicability and design of such analyses depend critically on the extent and pattern of linkage disequilibrium (LD) present in the study population. Cultivated barley (Hordeum vulgare ssp. vulgare) has many of the hallmarks known to be associated with a high level of LD. Its effective recombination rate is dramatically reduced by its predominantly inbreeding habit, with an estimated outcrossing rate of 5% in winter barley and ,0.5% in spring barley (Giles et al. 1974;Doll 1987;Abdel-Ghani et al. 2005). Domestication and intensive selection have introduced major bottlenecks in genetic variation, and these are thought to be largely responsible for the perceived narrowness of the modern gene pool (Badr et al. 2000;Russell et al. 2000;Matus and Hayes 2002). LD in modern spring barley, as estimated from a whole-genome survey, extends over distances of at least 10 cM, indicative of an extensive conservation of the genetic identity of barley chromosomes (Kraakman et al. 2004). However, estimates of genomewide LD conceal localized variation, which, as has been shown for a number of species, can be substantial and independent of the mating system (Gupta et al. 2005). In the self-pollinating species Arabidopsis thaliana LD varies from ,10 kb in global (Tian et al. 2002) to 50-250 kb in local populations (Nordborg et al. , 2005Aranzana et al. 2005). Within a given set of maize (an outbreeding species) accessions, the extent of LD has been documented to vary widely around different genes and chr...

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“…However, pathotypes of the soil-borne barley mosaic virus complex have been identified which have overcome rym4 (BaYMV-2, Huth 1989) and rym5 (BaMMV-Sil, Hariri et al 2003;Kanyuka et al 2004 andBaMMV-Teik, Habekuß et al 2008). Consequently, it is important to look for new, effective resistance genes from other sources including the secondary gene pool of barley.…”

Section: Discussionmentioning

confidence: 99%

Marker assisted separation of resistance genes Rph22 and Rym16 Hb from an associated yield penalty in a barley: Hordeum bulbosum introgression line

et al. 2015

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The resistance genes Rph22 and Rym16 (Hb) transferred into barley from Hordeum bulbosum have been separated from a large yield penalty locus that was present in the original introgression line '182Q20'. The Hordeum bulbosum introgression line '182Q20' possesses resistance to barley leaf rust (Rph22) and Barley mild mosaic virus (Rym16 (Hb) ) located on chromosome 2HL. Unfortunately, this line also carries a considerable yield penalty compared with its barley genetic background 'Golden Promise'. Quantitative trait locus (QTL) mapping of the components of yield (total yield, thousand grain weight, hectolitre weight, percentage screenings and screened yield) was performed using 75 recombinant lines derived from the original '182Q20' introgression line. A QTL for the yield penalty was located in the proximal region of the introgressed segment. Marker assisted selection targeting intraspecific recombination events between overlapping H. bulbosum introgression segments was used to develop the lines '372E' and '372H' which feature genetically small introgressions around Rph22. Further yield trials validated the separation of both Rph22 and Rym16 (Hb) from the proximal yield penalty. These results, combined with molecular markers closely linked to Rph22 and Rym16 (Hb) , make these resistance genes more attractive for barley breeding.

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Biological and sequence analysis of a novel European isolate of Barley mild mosaic virus that overcomes the barley rym5 resistance gene

Cited by 54 publications

References 32 publications

PROTEIN DISULFIDE ISOMERASE LIKE 5-1is a susceptibility factor to plant viruses

PROTEIN DISULFIDE ISOMERASE LIKE 5-1is a susceptibility factor to plant viruses

Effects of Introgression and Recombination on Haplotype Structure and Linkage Disequilibrium Surrounding a Locus EncodingBymovirusResistance in Barley

Marker assisted separation of resistance genes Rph22 and Rym16 Hb from an associated yield penalty in a barley: Hordeum bulbosum introgression line

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