Biological anolyte regeneration system for redox flow batteries

Simoska, Olja; Rhodes, Zayn; Carroll, Emily; Petrosky, Katia N; Minteer, Shelley D.

doi:10.1039/d2cc06011f

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…The general procedural protocol for immobilizing bacteria on the anode surfaces follows previously established experimental steps by our research group, reported elsewhere. ,− To characterize the anode half-cells, AvCarb carbon paper (AvCarb MGL 190, Fuel Cell Store) was cut to obtain working electrodes with an area of 1 cm 2 . For MFC setup and operation, carbon cloth electrodes (Carbon Cloth CC4 Plain, Fuel Cell Store) with an area of 6.5 cm 2 were used as the anode material.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…These SWV results show distinct redox potential signatures at −0.21 and −0.39 V versus SCE, which correspond to PYO and PCA, respectively, and agree with the redox potential values for these phenazines reported by previous research studies. 49,66 For these experiments, we took voltammetric measurements every hour for the initial 1−6 h, followed by every 2 h, followed by 13 h, and finally followed by 3 h of the 30-h bacterial growth period on the conductive carbon paper electrodes. As phenazine production by the genetically engineered E. coli cells increases, the peak currents for PCA and PYO in the CV and SWV data increase accordingly.…”

Section: Genetically Engineering Phenazine Biosynthesismentioning

confidence: 99%

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Enhancing the Performance of Microbial Fuel Cells via Metabolic Engineering of Escherichia coli for Phenazine Production

Simoska,

Cummings,

Gaffney

et al. 2023

ACS Sustainable Chem. Eng.

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One of the central roles in the design, development, and application advances in mediator-based microbial electrochemical systems, such as microbial fuel cells (MFCs), is the establishment of efficient and successful communication between conductive electrode surfaces and microorganisms via modes of extracellular electron transfer (EET). Most microbial-based systems require the use of artificial electroactive mediators in order to facilitate and/or enhance electron transfer. Our previous work established an exogenous phenazine-based library as a mediator system to enable EET from the model microorganism Escherichia coli as a promising biotechnological host. However, the addition of exogenous mediators to a microbial electrochemical system has certain limiting downsides, specifically with regard to mediator toxicity to cells and increased operational expenses. Herein, we demonstrate the metabolic and genetic engineering of E. coli to self-generate phenazine metabolites endogenously by introducing the phenazine biosynthetic pathway from P. aeruginosa into E. coli. This biosynthetic pathway contains a phenazine cluster of seven genes, namely, phzABCDEFG (phzA-G), responsible for the synthetic conversion of phenazine-1-carboxylic acid (PCA) from chorismic acid, and two additional phenazine accessory genes phzM and phzS to catalyze the transformation of PCA to pyocyanin (PYO). We present the characterization of the engineered E. coli cells that were collected via electrochemical measurements, RNA sequencing, and microscopy imaging. Finally, the engineered E. coli cells were used for the design of a microbial fuel cell with enhanced performances, demonstrating a maximum power density increase from 127 ± 5 mW m −2 with nonengineered E. coli cells to 806 ± 7 mW m −2 with genetically engineered, phenazine-producing E. coli. Our results indicate that the introduction of a heterologous electron shuttle into E. coli is not only an efficient, but also a promising strategy toward establishing efficacious electron mediation in living bioelectrochemical systems and enhancing the overall MFC performance related to the MFC current generation and power output.

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Section: Experimental Methodsmentioning

confidence: 99%

Section: Genetically Engineering Phenazine Biosynthesismentioning

confidence: 99%

Enhancing the Performance of Microbial Fuel Cells via Metabolic Engineering of Escherichia coli for Phenazine Production

Simoska,

Cummings,

Gaffney

et al. 2023

ACS Sustainable Chem. Eng.

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“…The possible chemical decomposition mechanisms of organic redox‐active materials include dimerization, tautomerization, hydrolysis, disproportionation, and nucleophilic addition/substitution 27,37,68,97,98 . In addition, biological electrolyte regeneration systems, such as the use of Escherichia coli to regenerate the degraded/decomposed redox‐active phenazines in the anolyte, may provide a possibility to further enhance the stability of RFBs 99 . So far, several recent reviews have well summarized the possible capacity degradation mechanisms of AORFBs 68–70 .…”

Section: Capacity Degradation Mechanism Studiesmentioning

confidence: 99%

“…27,37,68,97,98 In addition, biological electrolyte regeneration systems, such as the use of Escherichia coli to regenerate the degraded/decomposed redox-active phenazines in the anolyte, may provide a possibility to further enhance the stability of RFBs. 99 So far, several recent reviews have well summarized the possible capacity degradation mechanisms of AORFBs. [68][69][70] Interested readers can access these studies and reviews for more detailed information.…”

Section: Capacity Degradation Mechanism Studiesmentioning

confidence: 99%

Development of organic redox‐active materials in aqueous flow batteries: Current strategies and future perspectives

Pan

Shao

Jin³

2023

SmartMat

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Aqueous redox flow batteries, by using redox‐active molecules dissolved in nonflammable water solutions as electrolytes, are a promising technology for grid‐scale energy storage. Organic redox‐active materials offer a new opportunity for the construction of advanced flow batteries due to their advantages of potentially low cost, extensive structural diversity, tunable electrochemical properties, and high natural abundance. In this review, we present the emergence and development of organic redox‐active materials for aqueous organic redox flow batteries (AORFBs), in particular, molecular engineering concepts and strategies of organic redox‐active molecules. The typical design strategies based on organic redox species for high‐capacity, high‐stability, and high‐voltage AORFBs are outlined and discussed. Molecular engineering of organic redox‐active molecules for high aqueous solubility, high chemical/electrochemical stability, and multiple electron numbers as well as satisfactory redox potential gap between the redox pair is essential to realizing high‐performance AORFBs. Beyond molecular engineering, the redox‐targeting strategy is an effective way to obtain high‐capacity AORFBs. We further discuss and analyze the redox reaction mechanisms of organic redox species based on a series of electrochemical and spectroscopic approaches, and succinctly summarize the capacity degradation mechanisms of AORFBs. Furthermore, the current challenges, opportunities, and future directions of organic redox‐active materials for AORFBs are presented in detail.

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“…A wide body of literature exists on many different types of mediators and mediator modifications to tune their redox potential in accordance with the wide potential range of redox cofactors. Common redox mediators can include metal complexes and polymers (e. g. osmium), [45] metallocene molecules (e. g. ferrocene or cobaltocene), [20,46] quinones,, [47][48] phenazines, [49][50][51][52][53] and viologens [19,54] (Figure 6).…”

Section: Charge Transfer Mechanisms: Direct and Mediated Electron Tra...mentioning

confidence: 99%

Bioelectrocatalytic Synthesis: Concepts and Applications

et al. 2023

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Bioelectrocatalytic synthesis is the conversion of electrical energy into value‐added products using biocatalysts. These methods merge the specificity and selectivity of biocatalysis and energy‐related electrocatalysis to address challenges in the sustainable synthesis of pharmaceuticals, commodity chemicals, fuels, feedstocks and fertilizers. However, the specialized experimental setups and domain knowledge for bioelectrocatalysis pose a significant barrier to adoption. This review introduces key concepts of bioelectrosynthetic systems. We provide a tutorial on the methods of biocatalyst utilization, the setup of bioelectrosynthetic cells, and the analytical methods for assessing bioelectrocatalysts. Key applications of bioelectrosynthesis in ammonia production and small‐molecule synthesis are outlined for both enzymatic and microbial systems. This review serves as a necessary introduction and resource for the non‐specialist interested in bioelectrosynthetic research.

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Biological anolyte regeneration system for redox flow batteries

Abstract: Redox flow battery (RFB) electrolyte degradation is a common failure mechanism in RFBs. We report an RFB using genetically engineered, phenazine-producing Escherichia coli to serve as an anolyte regeneration system...

Cited by 6 publications

References 21 publications

Enhancing the Performance of Microbial Fuel Cells via Metabolic Engineering of Escherichia coli for Phenazine Production

Enhancing the Performance of Microbial Fuel Cells via Metabolic Engineering of Escherichia coli for Phenazine Production

Development of organic redox‐active materials in aqueous flow batteries: Current strategies and future perspectives

Bioelectrocatalytic Synthesis: Concepts and Applications

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