Biological characteristics and metabolic profile of canine mesenchymal stem cells isolated from adipose tissue and umbilical cord matrix

Marcoccia, Romina; Nesci, Salvatore; Merlo, Barbara; Ballotta, Giulia; Algieri, Cristina; Pagliarani, Alessandra; Iacono, Eleonora

doi:10.1371/journal.pone.0247567

Cited by 9 publications

(7 citation statements)

References 51 publications

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“…MSCs were successfully isolated from canine WJ for the first time by Seo and collaborators in 2012 [ 65 ]. These cells showed a typical mesenchymal immunophenotype and the ability to differentiate in vitro [ 65 ], as confirmed lately by other research groups [ 45 , 48 , 66 , 67 ] ( Table 1 ). Recently, Souza et al [ 66 ] demonstrated that the optimum conditions for canine WJMSCs osteogenic differentiation is co-culture with PRP and DBM, associated with optimum ALP levels and high levels of osteocalcin gene, a late marker of osteogenesis, and osteopontin gene, an important factor in bone remodeling [ 68 , 69 ].…”

Section: Canine Mesenchymal Stem Cells From Foetal Adnexasupporting

confidence: 72%

“…Recently, the metabolic profile of canine WJMSCs was investigated and compared with that of canine ATMSCs cultured under the basal conditions [ 67 ]. In order to detect the total ATP production rates in living MSCs, serial additions of oligomycin, a specific inhibitor of the mitochondrial ATP synthase, and of rotenone plus antimycin A, inhibitors of mitochondrial complex I and III, respectively, were performed automatically and stepwise.…”

Section: Canine Mesenchymal Stem Cells From Foetal Adnexamentioning

confidence: 99%

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Current Status on Canine Foetal Fluid and Adnexa Derived Mesenchymal Stem Cells

Iacono

Marcoccia

Merlo

2021

Animals

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: Effective standards of care treatment guidelines have been developed for many canine diseases. However, a subpopulation of patients is partially or completely refractory to these protocols, so their owners seek novel therapies such as treatments with MSCs. Although in dogs, as with human medicine, the most studied MSCs sources have been bone marrow and adipose tissue, in recent years, many researchers have drawn attention towards alternative sources, such as foetal adnexa and fluid, since they possess many advantages over bone marrow and adipose tissue. Foetal adnexa and fluid could be considered as discarded material; therefore, sampling is non-invasive, inexpensive and free from ethical considerations. Furthermore, MSCs derived from foetal adnexa and fluid preserve some of the characteristics of the primitive embryonic layers from which they originate and seem to present immune-modulatory properties that make them a good candidate for allo- and xenotransplantation. The aim of the present review is to offer an update on the state of the art on canine MSCs derived from foetal adnexa and fluid focusing on the findings in their clinical setting.

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Section: Canine Mesenchymal Stem Cells From Foetal Adnexasupporting

confidence: 72%

Section: Canine Mesenchymal Stem Cells From Foetal Adnexamentioning

confidence: 99%

Current Status on Canine Foetal Fluid and Adnexa Derived Mesenchymal Stem Cells

Iacono

Marcoccia

Merlo

2021

Animals

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“…The final concentration used for ATP Rate Assay were 1.5 μM oligomycin (port A) and 0.5 μM rotenone (Rot) plus antimycin A (AA) (port B). For the Cell Mito Stress Test, the final concentrations were 1.5 μM oligomycin (olig) (port A), 2.0 μM carbonyl-cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP (port B) with fibroblasts, 3.0 μM FCCP with preadipocytes, and 0.5 μM rotenone plus antimycin A (port C) [ 32 ]. In the Glycolytic Rate Assay, we used 0.5 μM rotenone plus antimycin A (rot + AA) (port a) and 50 mM 2-deoxy-D-glucose (2-DG), while for the Cell Energy Phenotype Test, the final concentrations were 1.5 μM oligomycin plus 2.0 μM, or 3.0 μM FCCP (port A) in presence of fibroblasts, preadipocytes, or adipocytes.…”

Section: Methodsmentioning

confidence: 99%

Cellular Metabolism and Bioenergetic Function in Human Fibroblasts and Preadipocytes of Type 2 Familial Partial Lipodystrophy

Algieri

Bernardini

Trombetti

et al. 2022

IJMS

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LMNA mutation is associated with type-2 familial partial lipodystrophy (FPLD2). The disease causes a disorder characterized by anomalous accumulation of body fat in humans. The dysfunction at the molecular level is triggered by a lamin A/C mutation, impairing the cell metabolism. In human fibroblasts and preadipocytes, a trend for ATP production, mainly supported by mitochondrial oxidative metabolism, is detected. Moreover, primary cell lines with FPLD2 mutation decrease the mitochondrial ATP production if compared with the control, even if no differences are observed in the oxygen consumption rate of bioenergetic parameters (i.e., basal and maximal respiration, spare respiratory capacity, and ATP turnover). Conversely, glycolysis is only inhibited in FPLD2 fibroblast cell lines. We notice that the amount of ATP produced in the fibroblasts is higher than in the preadipocytes, and likewise in the control, with respect to FPLD2, due to a more active oxidative phosphorylation (OXPHOS) and glycolysis. Moreover, the proton leak parameter, which characterizes the transformation of white adipose tissue to brown/beige adipose tissue, is unaffected by FPLD2 mutation. The metabolic profile of fibroblasts and preadipocytes is confirmed by the ability of these cell lines to increase the metabolic potential of both OXPHOS and glycolysis under energy required independently by the FPLD2 mutation.

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“…All parameter values were calculated per well, according to the manufacturer’s instructions. Both ATP Rate Assay, Mito Stress Test, and Cell Energy Phenotype Test were carried out three times in independent experiments ( Marcoccia et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Vitamin K Vitamers Differently Affect Energy Metabolism in IPEC-J2 Cells

Bernardini

Algieri

Mantia

et al. 2021

Front. Mol. Biosci.

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The fat-soluble vitamin K (VK) has long been known as a requirement for blood coagulation, but like other vitamins, has been recently recognized to play further physiological roles, particularly in cell development and homeostasis. Vertebrates cannot de novo synthesize VK, which is essential, and it can only be obtained from the diet or by the activity of the gut microbiota. The IPEC-J2 cell line, obtained from porcine small intestine, which shows strong similarities to the human one, represents an excellent functional model to in vitro study the effect of compounds at the intestinal level. The acute VK treatments on the bioenergetic features of IPEC-J2 cells were evaluated by Seahorse XP Agilent technology. VK exists in different structurally related forms (vitamers), all featured by a naphtoquinone moiety, but with distinct effects on IPEC-J2 energy metabolism. The VK1, which has a long hydrocarbon chain, at both concentrations (5 and 10 μM), increases the cellular ATP production due to oxidative phosphorylation (OXPHOS) by 5% and by 30% through glycolysis. The VK2 at 5 μM only stimulates ATP production by OXPHOS. Conversely, 10 μM VK3, which lacks the long side chain, inhibits OXPHOS by 30% and glycolysis by 45%. However, even if IPEC-J2 cells mainly prefer OXPHOS to glycolysis to produce ATP, the OXPHOS/glycolysis ratio significantly decreases in VK1-treated cells, is unaffected by VK2, and only significantly increased by 10 μM VK3. VK1, at the two concentrations tested, does not affect the mitochondrial bioenergetic parameters, while 5 μM VK2 increases and 5 μM VK3 reduces the mitochondrial respiration (i.e., maximal respiration and spare respiratory capacity). Moreover, 10 μM VK3 impairs OXPHOS, as shown by the increase in the proton leak, namely the proton backward entry to the matrix space, thus pointing out mitochondrial toxicity. Furthermore, in the presence of both VK1 and VK2 concentrations, the glycolytic parameters, namely the glycolytic capacity and the glycolytic reserve, are unaltered. In contrast, the inhibition of glycoATP production by VK3 is linked to the 80% inhibition of glycolysis, resulting in a reduced glycolytic capacity and reserve. These data, which demonstrate the VK ability to differently modulate IPEC-J2 cell energy metabolism according to the different structural features of the vitamers, can mirror VK modulatory effects on the cell membrane features and, as a cascade, on the epithelial cell properties and gut functions: balance of salt and water, macromolecule cleavage, detoxification of harmful compounds, and nitrogen recycling.

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Biological characteristics and metabolic profile of canine mesenchymal stem cells isolated from adipose tissue and umbilical cord matrix

Cited by 9 publications

References 51 publications

Current Status on Canine Foetal Fluid and Adnexa Derived Mesenchymal Stem Cells

Current Status on Canine Foetal Fluid and Adnexa Derived Mesenchymal Stem Cells

Cellular Metabolism and Bioenergetic Function in Human Fibroblasts and Preadipocytes of Type 2 Familial Partial Lipodystrophy

Vitamin K Vitamers Differently Affect Energy Metabolism in IPEC-J2 Cells

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