Biological Characteristics and Viral Susceptibility of an African Green Monkey Kidney Cell Line (Vero)

Rhim, Johng S.; Schell, Klaus; Creasy, B.; Case, William

doi:10.3181/00379727-132-34285

Cited by 65 publications

(36 citation statements)

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“…HaCat is an immortalized human keratinocyte cell line that bears mutated alleles of p53 (2,22), HEp-2 is a human laryngeal carcinoma cell line (ATCC), and HeLa is a human cervix adenocarcinoma cell line (ATCC). Vero is an African green monkey kidney cell line (36), and BHK cells are baby hamster kidney cells (18). All cell lines, with the exception of HeLa cells, were grown in RPMI 1640 medium supplemented with 10% fetal calf serum.…”

Section: Methodsmentioning

confidence: 99%

Infectious Epstein-Barr Virus Lacking Major Glycoprotein BLLF1 (gp350/220) Demonstrates the Existence of Additional Viral Ligands

Janz

Oezel

Kurzeder³

et al. 2000

J Virol

151

134

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The binding of the viral major glycoprotein BLLF1 (gp350/220) to the CD21 cellular receptor is thought to play an essential role during infection of B lymphocytes by the Epstein-Barr virus (EBV). However, since CD21-negative cells have been reported to be infectible with EBV, additional interactions between viral and cellular molecules seem to be probable. Based on a recombinant genomic EBV plasmid, we deleted the gene that encodes the viral glycoprotein BLLF1. We tested the ability of the viral mutant to infect different lymphoid and epithelial cell lines. Primary human B cells, lymphoid cell lines, and nearly all of the epithelial cell lines that are susceptible to wild-type EBV infection could also be successfully infected with the viral mutant in vitro, although the efficiency of infection with BLLF1-negative virus was clearly lower than the one observed with wild-type EBV. Our studies show that the interaction between BLLF1 and CD21 is not absolutely required for the infection of lymphocytes and epithelial cells, indicating that viral molecules other than BLLF1 can mediate the binding of EBV to its target cells. In this context, our results further suggest the hypothesis that additional cellular molecules, apart from CD21, allow virus entry into these cells.

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Section: Methodsmentioning

confidence: 99%

Infectious Epstein-Barr Virus Lacking Major Glycoprotein BLLF1 (gp350/220) Demonstrates the Existence of Additional Viral Ligands

Janz

Oezel

Kurzeder³

et al. 2000

J Virol

151

134

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“…Vero cells (18) obtained from the American Type Culture Collection were cultured in 199 Earle's medium supplemented with 5% fetal bovine serum and 40 µg/ml gentamicin.…”

Section: Cells and Virusesmentioning

confidence: 99%

Comparative sequence analysis of the P-, M- and L-coding region of the measles virus CAM-70 live attenuated vaccine strain

Santos

Azevedo

Borges

et al. 2003

Braz J Med Biol Res

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Measles virus is a highly contagious agent which causes a major health problem in developing countries. The viral genomic RNA is singlestranded, nonsegmented and of negative polarity. Many live attenuated vaccines for measles virus have been developed using either the prototype Edmonston strain or other locally isolated measles strains. Despite the diverse geographic origins of the vaccine viruses and the different attenuation methods used, there was remarkable sequence similarity of H, F and N genes among all vaccine strains. CAM-70 is a Japanese measles attenuated vaccine strain widely used in Brazilian children and produced by Bio-Manguinhos since 1982. Previous studies have characterized this vaccine biologically and genomically. Nevertheless, only the F, H and N genes have been sequenced. In the present study we have sequenced the remaining P, M and L genes (approximately 1.6, 1.4 and 6.5 kb, respectively) to complete the genomic characterization of CAM-70 and to assess the extent of genetic relationship between CAM-70 and other current vaccines. These genes were amplified using long-range or standard RT-PCR techniques, and the cDNA was cloned and automatically sequenced using the dideoxy chain-termination method. The sequence analysis comparing previously sequenced genotype A strains with the CAM-70 Bio-Manguinhos strain showed a low divergence among them. However, the CAM-70 strains (CAM-70 Bio-Manguinhos and a recently sequenced CAM-70 submaster seed strain) were assigned to a specific group by phylogenetic analysis using the neighbor-joining method. Information about our product at the genomic level is important for monitoring vaccination campaigns and for future studies of measles virus attenuation.

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“…A qualified WHO Vero cell bank was established for the production of biological products 40 and methods for large-scale vaccine production are well established. 41,42 Furthermore, Vero cells are highly permissive to viral infection, 43 which is likely caused by a defect in type I IFN production. 44 Previous attempts have been made to adapt JEV SA14-14-2 to more suitable cell substrates and have resulted in mutations in the virus that reduced vaccine immunogenicity.…”

Section: Introductionmentioning

confidence: 99%

Genetic and Phenotypic Properties of Vero Cell-Adapted Japanese Encephalitis Virus SA14-14-2 Vaccine Strain Variants and a Recombinant Clone, Which Demonstrates Attenuation and Immunogenicity in Mice

Gromowski

Firestone

Bustos-Arriaga

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. The live-attenuated Japanese encephalitis virus (JEV) SA14-14-2 vaccine, produced in primary hamster kidney cells, is safe and effective. Past attempts to adapt this virus to replicate in cells that are more favorable for vaccine production resulted in mutations that significantly reduced immunogenicity. In this study, 10 genetically distinct Vero cell-adapted JEV SA14-14-2 variants were isolated and a recombinant wild-type JEV clone, modified to contain the JEV SA14-14-2 polyprotein amino acid sequence, was recovered in Vero cells. A single capsid protein mutation (S66L) was important for Vero cell-adaptation. Mutations were also identified that modulated virus sensitivity to type I interferonstimulation in Vero cells. A subset of JEV SA14-14-2 variants and the recombinant clone were evaluated in vivo and exhibited levels of attenuation that varied significantly in suckling mice, but were avirulent and highly immunogenic in weanling mice and are promising candidates for the development of a second-generation, recombinant vaccine.

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Biological Characteristics and Viral Susceptibility of an African Green Monkey Kidney Cell Line (Vero)

Cited by 65 publications

References 0 publications

Infectious Epstein-Barr Virus Lacking Major Glycoprotein BLLF1 (gp350/220) Demonstrates the Existence of Additional Viral Ligands

Infectious Epstein-Barr Virus Lacking Major Glycoprotein BLLF1 (gp350/220) Demonstrates the Existence of Additional Viral Ligands

Comparative sequence analysis of the P-, M- and L-coding region of the measles virus CAM-70 live attenuated vaccine strain

Genetic and Phenotypic Properties of Vero Cell-Adapted Japanese Encephalitis Virus SA14-14-2 Vaccine Strain Variants and a Recombinant Clone, Which Demonstrates Attenuation and Immunogenicity in Mice

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