Biological characteristics of maize dwarf mosaic potyvirus from Spain

Abstract

Two isolates of maize dwarf mosaic virus (MDMV-Sp and MDMV-Spl) obtained from maize in the northeast of Spain were studied. Both isolates induced similar reactions on 6 sorghum cultivars, johnsongrass and oat (cv. Clintland), with the exception of MDMV-Sp which produced a different reaction on one sorghum cultivar. Thirtythree grass species were tested as possible hosts (16 previously untested) and 18 were found to be susceptible. Among those, eight were previously unidentified hosts for MDMV: Aegilops ovata, A. ventricosa, Avena barbata, Bromus alopecurus, B. diandrus, B. fasciculatus, Echinaria capitata and Lolium rigidum. Both isolates were transmitted from maize to maize nonpersistently by Rhopalosiphum maidis, R. padi, Schizaphis graminum and Sitobium avenae. The virus was not seed-transmitted in Mo17 and B73 maize inbred lines. Pinwheels, scrolls and short curved laminated aggregates were observed in the cytoplasm of maize cells infected by MDMV-Sp or MDMV-Spl. In addition, laminated aggregates were observed in cells infected by MDMV-Spl.

Impact of 9 years of Bt-maize cultivation on the distribution of maize viruses

“…In this sense, it is remarkable that Sitobion avenae was the aphid species with the highest densities on Bt-maize compared to non-Bt-maize. This aphid transmits SCMV (42%) (Achon et al 2003) more efficiently than MDMV (6%) (Achon et al 1996). It will be necessary to study the relation between S. avenae populations and SCMV incidence over a longer period in order to confirm the positive effect of this aphid on the increasing presence of SCMV in Spain.…”

“…All samples were analyzed with polyclonal antisera against MDMV-Sp. (Achon et al 1996), SCMV (Loewe GmbH) and MRDV (Bio-Rad, Hercules, CA). Immunoglobulin (Ig)Gs from commercial antisera were used at the dilution and in the buffers recommended by the manufacturer, while in the same buffers, those isolated from antisera obtained in our laboratory were used at 0.1 lg/ml and alkaline phosphatase-conjugated IgGs were used at a dilution of 1:1,000.…”

Maize dwarf mosaic virus diversity in the Johnsongrass native reservoir and in maize: evidence of geographical, host and temporal differentiation

“…To assess the putative bottleneck of aphid transmission in the MDMV population, haplotypes E and Y, which had both been obtained from Johnsongrass, were mechanically inoculated onto maize plants as described in Achon et al. (1996), and the resulting infected plants used to feed aphids (Table 8).…”

“…To assess the putative bottleneck of aphid transmission in the MDMV population, haplotypes E and Y, which had both been obtained from Johnsongrass, were mechanically inoculated onto maize plants as described in Achon et al (1996), and the resulting infected plants used to feed aphids (Table 8). Transmission was carried out in a nonpersistent manner and transmission rates were determined serologically as indicated in Achon et al (2003).…”

Untersuchungen zum wirtskreis des sugarcane mosaicpotyvirus(scmv) und des maize dwarf mosaicpotyvirus(MDMV) in Deutschland*

“…Möglicherweise stellt der Nachweis von Waldgerste (H. europaeus) und Hundsquecke (R. canina) als perennierende Wirte für SCMV einen Hinweis in diese Richtung dar. Die zuerst genannte Art wurde bisher noch nie als Wirtspflanze beschrieben, die letztere bei experimenteller Prüfung als Nichtwirt aufgeführt (Huth und Lesemann, 1991;Achon, 1996). Waldgerste ist eine ausgesprochen basenholde Art, die zerstreut in Laubmischwäldern und an Waldsäumen vorkommt.…”