Biological characteristics of newly described HIV-1 BG recombinants in Spanish individuals

Pérez-Álvarez, Lucı́a; Delgado, Elena; Villahermosa, Marı́a Luisa; Cuevas, Maria Teresa; Garcia, Valentina E.; Parga, Elena Vázquez de; Thomson, Michael M.; Prieto, Á.; Cuevas, Laureano; Medrano, Leandro; Taboada, José Antonio Franco; Nájera, Rafael

doi:10.1097/00002030-200203080-00021

Cited by 13 publications

(18 citation statements)

References 18 publications

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“…The phenotype of the newly defined CRF14_BG has been described as syncytium inducing, with usage of CXCR4, CCR5, CCR3, and CCR2b coreceptors. 21 In this study we characterized the full-length genome of the mosaic HIV-1 isolate NARI-FLS_09-387. The recombinant isolate shows insertion of subtype A1 at four positions in the backbone of subtype C. Clustering of the subtype C portion with Indian HIV-1 isolates suggests its Indian origin.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Unique Intersubtype HIV Type 1 A1/C Recombinant Strain Circulating in Pune, India

Pandey

Tripathy²,

Paranjape³

2013

AIDS Research and Human Retroviruses

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An increasing number of circulating recombinant forms (CRFs) and unique recombinant forms (URFs) all over the world has necessitated being vigilant about new recombinants. Since the first report of a recombinant virus with an A1/C mosaic in 1998 more and more B/C and A/C recombinant viruses are being reported from India. Here we report the identification and characterization of a unique HIV-1 A1/C recombinant circulating in Western India. Analysis of the full-length genome using RIP, SimPlot, and jpHMM@Gobics has confirmed its mosaic structure with insertion of subtype A1 in the backbone of subtype C at three positions: gag-pol (1973 -15-2617 -47), pol-vif (4879 -37-5582 -32), and gp41 (8437 -106-8811 -8); however, RIP and SimPlot showed one more small insertion in integrase (4343-4519). Phylogenetic analysis confirmed that the recombinant virus has an insertion of clade A1 in the backbone of subtype C, which has come from Indian subtype C.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Unique Intersubtype HIV Type 1 A1/C Recombinant Strain Circulating in Pune, India

Pandey

Tripathy²,

Paranjape³

2013

AIDS Research and Human Retroviruses

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“…The results of phylogenetic analysis of some of these samples were described previously [Thomson et al, 2001;Pérez-Alvarez et al, 2003]. Twelve viruses were characterized with full-length sequences from plasma RNA [Delgado et al, 2002;Casado et al, 2003;Sierra et al, 2005].…”

Section: Methodsmentioning

confidence: 99%

Isolation and biological characterization of HIV-1 BG intersubtype recombinants and other genetic forms circulating in Galicia, Spain

et al. 2006

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The biological characteristics of HIV-1 primary isolates of different recombinant forms (RFs) and non-B subtypes from Galicia, Spain, were investigated and the relationships between biological phenotype and evolution of infection were determined. Peripheral blood mononuclear cells (PBMCs) were obtained during the follow-up of 32 patients infected with HIV-1 non-subtype B genetic forms, characterized in partial sequences of pol (protease-reverse transcriptase) and env V3 region: 12 (37.5%) circulating RFs (CRFs), 9 (28.1%) unique RFs (URFs), and 11(34.4%) non-B subtypes. Primary isolates were obtained by coculture with donor PBMCs. Syncytium-inducing (SI) phenotype was examined in MT2 cell line and coreceptor use in GHOST and U87.CD4 cells. Fifty percent of tissue culture infective dose (TCID(50)) and viral phenotype based on V3 net charge and Geno2pheno(coreceptor) bioinformatic method were determined. Fifty-four HIV-1 primary isolates were obtained. CRF14_BG and BG URFs represented the largest group, being all SI/X4, independently of the CD4+ cell count, viral load, or the duration of infection. By contrast, 10 of 11 CRF02_AG viruses were NSI/R5. The prediction of co-receptor use was concordant with biological characterization in all NSI/R5 and in 23 of 26 SI/X4 isolates. The presence of SI/X4 or SI/X4,R5 isolates at early stages of the infection in addition to a decrease in CD4+ counts below 500 cells/microl between 2 and 6 years since diagnosis was observed in all patients infected with CRF14_BG and BG URFs. These data contrast with the usual progression in B subtype infections, in which SI/X4 viruses rarely predominate in the early years of HIV-1 infection.

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“…In contrast to subtype C, investigation of the biological characteristics of CRF14_BG from Galicia, Spain revealed an SI phenotype for these strains, even though they were obtained from a patient in the early A1 clinical stage; in addition, utilization of CCR2b, CCR3, CCR5 or CXCR4 as coreceptors as well as a characteristic pattern of mutations in V3 were also discovered [328]. On the contrary, unique recombinant forms co-circulating in the area have been found to display a NSI/CCR5 phenotype that may provide these strains with a selective advantage for heterosexual transmission [328].…”

Section: Subtype Diversity and Biological Properties Of Hiv: Viral Phmentioning

confidence: 99%

“…On the contrary, unique recombinant forms co-circulating in the area have been found to display a NSI/CCR5 phenotype that may provide these strains with a selective advantage for heterosexual transmission [328]. According to Spira et al subtype A viruses also tend to favor utilization of CCR5, even at later stages of the disease course [405].…”

Section: Subtype Diversity and Biological Properties Of Hiv: Viral Phmentioning

confidence: 99%

“…Non-B HIV-1 subtypes that prevail in Africa and Asia bear more significance than subtype B, the predominant clade in Western and Central Europe, North America, and Australia, in the molecular epidemiology of the pandemic {reviewed in [6,113,328,405]}. In the year 2000, the largest proportion of all HIV-1 infections worldwide was due to subtype C strains (47.2%), while subtype A/CRF02_AG constituted the second leading cause of all infections (27%), followed by subtype B strains (12.3%) [319].…”

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confidence: 99%

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Viral Correlates of HIV-1 Disease

Anastassopoulou

Kostrikis²

2005

CHR

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The transmission of HIV and the progression of HIV disease are influenced not only by a large number of human host factors, but also by certain correlates of the ever fluctuating virus quasispecies. The present review article aims at providing the current state of knowledge as well as an in-depth critical discussion of recent developments on the potential effects of HIV subtype, phenotype and attenuation on HIV disease. Despite the extensive research, several questions regarding the precise role that each of these correlates plays in human AIDS pathogenesis remain unanswered. Unraveling these roles is expected to aid the continued quest for truly effective antiretroviral drug regimens and preventive vaccines.

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Biological characteristics of newly described HIV-1 BG recombinants in Spanish individuals

Cited by 13 publications

References 18 publications

Molecular Characterization of Unique Intersubtype HIV Type 1 A1/C Recombinant Strain Circulating in Pune, India

Molecular Characterization of Unique Intersubtype HIV Type 1 A1/C Recombinant Strain Circulating in Pune, India

Isolation and biological characterization of HIV-1 BG intersubtype recombinants and other genetic forms circulating in Galicia, Spain

Viral Correlates of HIV-1 Disease

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