Biological characterization of adipose‐derived regenerative cells used for the treatment of stress urinary incontinence

Abstract

ObjectiveTo assess the characteristics of adipose‐derived regenerative cells, and provide supportive data explaining the mechanism of efficacy observed for the use of these cells in the treatment of stress urinary incontinence.MethodsAdipose tissues were harvested by abdominal liposuction from healthy donors and patients with stress urinary incontinence. Adipose‐derived regenerative cells were isolated from tissues using the Celution system, and assessed for their characteristics and ability to differentiate into smooth muscle cells.ResultsAdipose‐derived regenerative cells isolated by the Celution system developed into fibroblastic colonies. Flow cytometric analysis of adipose‐derived stem cell markers showed that adipose‐derived regenerative cells were positive for CD34 and CD44, and negative for CD31. Immunofluorescence staining after differentiation showed that colony‐forming cells were positive for alpha‐smooth muscle actin, calponin and desmin, which are smooth muscle cell markers. A cytokine release assay showed that adherent cells secreted cytokines associated with angiogenesis, including vascular endothelial growth factor‐A, angiopoietin‐2 and placental growth factor.ConclusionsAdipose‐derived regenerative cells collected by the Celution system might have clonogenic capacity and an angiogenetic function. These properties might contribute to the mechanisms through which regenerative cell therapy by periurethral injection of autologous adipose‐derived regenerative cells ameliorates stress urinary incontinence.

Comparison of urethral sling surgery and non-ablative vaginal Erbium:YAG laser treatment in 327 patients with stress urinary incontinence: a case-matching analysis

“…Recently, the important role of blood flow in the urethra and bladder has been noted [37]. Stem cells have been used in treatment of stress urinary incontinence [38]; in a study using adipose-derived regenerative cells, a cytokine release assay showed that adherent cells secreted cytokines associated with angiogenesis, including vascular endothelial growth factor-A, angiopoietin-2, and placental growth factor. Using a stem cell Fig.…”

“…Recently, the important role of blood flow in the urethra and bladder has been noted [ 37 ]. Stem cells have been used in treatment of stress urinary incontinence [ 38 ]; in a study using adipose-derived regenerative cells, a cytokine release assay showed that adherent cells secreted cytokines associated with angiogenesis, including vascular endothelial growth factor-A, angiopoietin-2, and placental growth factor. Using a stem cell therapeutic approach [ 39 ] for pelvic floor disorders (PFD), studies using different autologous stem cells have achieved promising results by improving the pelvic ligament and muscle regeneration and conferring tissue elasticity and strength to the damaged tissue in PFD, as well as reducing inflammatory reactions, collagen deposition, and foreign body reaction [ 39 ].…”

Tryptanthrin inhibits tumor angiogenesis via Notch/Dll4 signaling pathway in zebrafish

“…Desmin is a smooth muscle intermediate filament protein expressed in fibrotic tissue associated with tumor “adhesion hyperplasia” wound healing and matrix. Additionally, desmin was described as a pericyte marker, which was associated with blood vessels from early stages of capillary germination throughout the entire angiogenesis process ( 27 , 28 ). Therefore, desmin may represent a biomarker for predicting the efficacy of anti-angiogenic therapy for cancer in our future study.…”

Periodontal tissue regeneration by transplantation of autologous adipose tissue-derived multi-lineage progenitor cells

“…Data were collected with a FACSCalibur (BD Biosciences). A colony forming assay was performed as previously described 34 . Briefly, 250 or 500 ADMPCs were seeded on a 6-well plate and cultured for 2 weeks.…”