Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care

Moore, Marc C.; Bonvallet, Paul P.; Damaraju, Sita M.; Modi, Heli N; Gandhi, Ankur; McFetridge, Peter S.

doi:10.1002/jbm.b.34635

Cited by 13 publications

(9 citation statements)

References 42 publications

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“…Amnion is often used solely or together with chorion to increase the thickness and the bioactive contents of the graft ( Koob et al, 2015 ). The possible inside mechanisms were indicated to be the bioactive factors contained within amnion and chorion tissues, including growth factors, cytokines, chemokines, and matrix metalloproteinases (MMPs) ( Choi et al, 2009 ; Rodriguez-Ares et al, 2009 ; Koob et al, 2013 ; Koob et al, 2014 ; Koob et al, 2015 ; Moore et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

A minimally manipulated preservation and virus inactivation method for amnion/chorion

Zhang¹,

Gao²,

Wang³

et al. 2022

Front. Bioeng. Biotechnol.

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Allogeneic amnion tissues have been widely used in tissue repair and regeneration, especially a remarkable trend of clinical uses in chronic wound repair. The virus inactivation procedures are necessary and required to be verified for the clinical use and approval of biological products. Cobalt-60 (Co-60) or electron-beam (e-beam) is the common procedure for virus and bacterial reduction, but the excessive dose of irradiation was reported to be harmful to biological products. Herein, we present a riboflavin (RB)-ultraviolet light (UV) method for virus inactivation of amnion and chorion tissues. We used the standard in vitro limiting dilution assay to test the viral reduction capacity of the RB-UV method on amnion or chorion tissues loaded with four types of model viruses. We found RB-UV was a very effective procedure for inactivating viruses of amnion and chorion tissues, which could be used as a complementary method to Co-60 irradiation. In addition, we also screened the washing solutions and drying methods for the retention of growth factors.

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Section: Introductionmentioning

confidence: 99%

A minimally manipulated preservation and virus inactivation method for amnion/chorion

Zhang¹,

Gao²,

Wang³

et al. 2022

Front. Bioeng. Biotechnol.

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“…In this study, the PCL-amnion nanofibrous membrane consists of PCL in the outer layer and amniotic membrane in the inner layer. The amniotic membrane can slowly release TGF-β1, bFGF, PDGF, and NGF cytokines (52). These cytokines continuously diffuses through the pore structure of PCL to the nerve repair site and promotes the proliferation of Schwann cells and axon regeneration.…”

Section: Discussionmentioning

confidence: 99%

Electrospun Polycaprolactone (PCL)-Amnion Nanofibrous Membrane Promotes Nerve Regeneration and Prevents Fibrosis in a Rat Sciatic Nerve Transection Model

et al. 2022

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Functional recovery after peripheral nerve injury repair is typically unsatisfactory. An anastomotically poor microenvironment and scarring at the repair site are important factors impeding nerve regeneration. In this study, an electrospun poly-e-caprolactone (PCL)-amnion nanofibrous membrane comprising an amnion membrane and nonwoven electrospun PCL was used to wrap the sciatic nerve repair site in the rat model of a sciatic nerve transection. The effect of the PCL-amnion nanofibrous membrane on improving nerve regeneration and preventing scarring at the repair site was evaluated by expression of the inflammatory cytokine, sciatic functional index (SFI), electrophysiology, and histological analyses. Four weeks after repair, the degree of nerve adhesion, collagen deposition, and intraneural macrophage invasion of the PCL-amnion nanofibrous membrane group were significantly decreased compared with those of the Control group. Moreover, the PCL-amnion nanofibrous membrane decreased the expression of pro-inflammatory cytokines such as interleukin(IL)-6, Tumor Necrosis Factor(TNF)-a and the number of pro-inflammatory M1 macrophages, and increased the expression of anti-inflammatory cytokine such as IL-10, IL-13 and anti-inflammatory M2 macrophages. At 16 weeks, the PCL-amnion nanofibrous membrane improved functional recovery, including promoting nerve Schwann cell proliferation, axon regeneration, and reducing the time of muscle denervation. In summary, the PCL-amnion nanofibrous membrane effectively improved nerve regeneration and prevent fibrosis after nerve repair, which has good clinical application prospect for tissue repair.

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“…Histological procedures have been described previously in detail. 16 Briefly, three different lots of TPAM were rehydrated in phosphate buffered saline (PBS) before histology processing. TPAM was then embedded in paraffin and sectioned.…”

Section: Methodsmentioning

confidence: 99%

“…[9][10][11][12] Some of the marked features of these growth factors are their ability to influence proliferation, migration, and ECM secretion from cells such as fibroblasts, in addition to support angiogenic, anti-inflammatory, and immunomodulatory functions. [13][14][15][16] Clinical case studies utilizing placental membranes for up to 8 weeks in burns and complex wounds as a biological dressing have demonstrated decreased in-flammation, reduced pain and scarring, and enhanced re-epithelialization. 17,18 The inherent bioactive properties and favorable clinical findings further reinforce the therapeutic potential of placental membranes as a therapy for chronic wound healing and tissue repair.…”

Section: Introductionmentioning

confidence: 99%

“… 24 Other studies have investigated the in vitro biological efficacy of amnion alone and amnion/chorion membranes using different cell lines to demonstrate wound healing applications. 9 , 16 , 25 , 26 However, the biophysical properties of these various membranes can limit their clinical utility. When hydrated, a placental membrane often becomes self-adherent and lacks mechanical rigidity that results in compromised handling properties and is difficult to position on a wound bed.…”

Section: Introductionmentioning

confidence: 99%

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Biophysical Characterization of a Novel Tri-Layer Placental Allograft Membrane

Bonvallet

Damaraju

Modi

et al. 2022

Advances in Wound Care

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Objective: Placental tissues, including membranes composed of amnion and chorion, are promising options for the treatment of chronic wounds. Amnion and chorion contain multiple extracellular matrix (ECM) proteins and a multitude of growth factors and cytokines that, when used clinically, assist in the progression of difficult to heal wounds through restoration of a normal healing process. The objective of this study was to characterize the in vitro physical and biological properties of a dehydrated tri-layer placental allograft membrane (TPAM) consisting of a chorion layer sandwiched between two layers of amnion. Approach: Mechanical properties were evaluated by mechanical strength and enzyme degradation assays. The ECM composition of TPAM membranes was evaluated by histological staining while growth factors and cytokine presence was evaluated by a multiplex enzyme-linked immunosorbent assay. Proliferation, migration, and ECM secretion assays were performed with fibroblasts. Immunomodulatory properties were assessed by a pro-inflammatory cytokine reduction assay while the macrophage phenotype was determined by quantifying the ratio of M1 versus M2 secreted factors. Results: The unique three-layer construction improves mechanical handling properties over single-and bi-layer membranes. Results demonstrate that TPAM is rich in ECM proteins, growth factors, cytokines, and tissue inhibitors of metalloproteinases, and favorably influences fibroblast migration, proliferation, and ECM secretion when compared to negative controls. Furthermore, after processing and preservation, these membranes maintain their intrinsic immunomodulatory properties with the ability to suppress pro-inflammatory processes and modulate the M1 and M2 macrophage phenotype toward a pro-regenerative profile when compared to a negative control. Innovation: This is the first study to characterize both the biophysical and biological properties of a tri-layer placental membrane. Conclusion: This work demonstrates that TPAM has improved handling characteristics over single-and bi-layer membranes, stimulates pro-healing cellular responses, and advantageously modulates inflammatory responses, altogether making this scaffold a promising option for treating wounds, especially those that are complex or difficult to heal.

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Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care

Cited by 13 publications

References 42 publications

A minimally manipulated preservation and virus inactivation method for amnion/chorion

A minimally manipulated preservation and virus inactivation method for amnion/chorion

Electrospun Polycaprolactone (PCL)-Amnion Nanofibrous Membrane Promotes Nerve Regeneration and Prevents Fibrosis in a Rat Sciatic Nerve Transection Model

Biophysical Characterization of a Novel Tri-Layer Placental Allograft Membrane

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