Biological characterization of emerging fungal pathogen Colletotrichum associated with mango (Mangifera indica L.) postharvest anthracnose from Vietnam

Abstract

Aims: To elucidate the taxonomic status and biological characteristics of the pathogens causing anthracnose of postharvest mango fruit (cultivar Okrong) from Vietnam. Methods and Results：Based on the morphological features, along with the phylogenetic tree of ITS and ApMat combined sequences, two strains DM-1 and DM-2 isolated from the fruit of Okrong from Vietnam were identified as Colletotrichum fructicola and C. asianum respectively, which were the pathogens of postharvest fruit anthracnose of mango from Vietnam. The growth status of different Colletotrichum strains under different conditions were analyzed to reveal the biological characteristics of different pathogens. The optimum growth temperature of DM-1 and DM-2 was 28 °C with mycelia grow fastest in the dark. Both strains could grow when pH was 4-11, and the optimum pH value was 6. Maltose and soluble starch were the most suitable carbon source for DM-1 and DM-2 respectively, and the peptone was the most suitable nitrogen source for both strains. The lethal temperatures were recorded as 55 °C 5 min for DM-1, and 50 °C 10 min for DM-2. Conclusions: To the best of our knowledge, It is the first time to confirmed that the pathogens of postharvest fruit anthracnose of mango from Vietnam were C. fructicola and C. asianum with different biological characteristics.