Biological characterization of mesenchymal stem cells from bovine umbilical cord

Abstract

Mesenchymal stem cells (MSCs) are multi-potential cells that are able to proliferate and differentiate into other cell types. Much research has been done on the MSCs from the umbilical cord (UCMSCs) in human, mice, and avian, but little literature has been published about these cells in big livestock. Here, we choose Luxi cattle as the experimental animal, we describe an external culture of the UCMSCs from it and summarize the biological characteristics of these cells, e.g., morphologic appearance, surface antigens, colony-forming ability, gene expression, and differentiation potential were detected via using immunofluorescence and reverse transcription polymerase chain reaction (RT-PCR). The induced cells, osteoblast, lipoblast, hepatocyte, islet cells, and neurocyte were identified by Alizarin red staining, Oil-red-O staining, Periodic acid-schiff staining, and Dithizone staining and RT-PCR detection for specific genes. Results suggest that biological characteristics of the UCMSCs were similar to those of MSCs previously analyzed. The primary UCMSCs were sub-cultured to passage 32, the UCMSCs expressed gene CD29, CD44, CD73, CD90, and CD166, induced cells illustrated typical staining, and expressed specific genes, which indicate that the UCMSCs could be a novel alternative source of MSCs for experimental and clinical applications.

Shotgun proteomic analysis of the secretome of bovine endometrial mesenchymal progenitor/stem cells challenged or not with bacterial lipopolysaccharide

“…Immunophenotypic evaluation by FC revealed high expression for the markers vimentin (94.35% ± 2.19), CD-29 (99.85% ± 0.07) and CD-44 (96.9% ± 2.40). Similar to previous studies (Xiong et al, 2014), there was low expression of the CD-34 (4.25% ± 1.06) marker, and no expression of the MHC-II marker (1.05% ± 0.78) (Fig. 2).…”

Heat Shock Alters Mesenchymal Stem Cell Identity and Induces Premature Senescence

“…The objectives of this study are to characterize the immunomodulatory properties of bovine umbilical cord (bUC) MSCs and to examine the effect of high temperature [e.g., heat shock (HS)] on their function. MSCs of bovine origin have been previously isolated and characterized from different tissues (Bosnakovski et al, 2005;Cabezas et al, 2014;Mançanares et al, 2015;Xiong et al, 2016). In utero thermoregulation is dependent on the mother's core temperature, and maternal heat stress can impact the fetal temperature through the fetal-placental circulation (Dado-Senn et al, 2020).…”

Bovine endometrial cells: a source of mesenchymal stem/progenitor cells

“…Additionally, CE revealed great capacity of clonicity from bovine eMSCs and the formation of delimitated colonies ( Figure 4A) without difference (P ¼ 0.750) between phases. This CE rate is excellent and comparable to samples from umbilical cords or adipose tissue Xiong et al, 2014). Therefore, creating cell biobanks for future use is quite feasible once eMSCs are stable and suitable for therapeutic use.…”

“…The significant marking in both immunophenotypic and immunocytochemistry evaluation for CD44 and vimentin seen in our study were previously described in bovine amniotic fluid‐derived MSCs (Rossi et al, ). The CD44 and CD29 expression was reported in porcine eMSC (Miernik and Karasinski, ), bovine umbilical cord (Xiong et al, ), and human eMSCs (Gargett et al, ; Verdi et al, ). The absence of marking for MHC‐II here was maybe due to low immunogenicity of the endometrial cells, which is of great interest for therapeutic use.…”