Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines

Bonfim-Silva, Ricardo; Salomão, Karina Bezerra; Pimentel, Thais Valéria Costa de Andrade; Menezes, Camila Cristina Branquinho de Oliveira; Palma, Patrícia Vianna Bonini; Fontes, Aparecida Maria

doi:10.1007/s10616-019-00332-3

Cited by 8 publications

(6 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conversely, Daoy MB cell proliferation and migration were significantly compromised with RBM5 overexpression. The Daoy cell line was reported previously to show a high migration potential [18], and in the present study, we found that the migration ability of Daoy cells increased after RBN5 knockdown. These findings are consistent with those observed in glioma tissues [13].…”

Section: Discussionsupporting

confidence: 80%

RBM5 Acts as Tumor Suppressor in Medulloblastoma through Regulating Wnt/β-Catenin Signaling

Wei

et al. 2020

Eur Neurol

View full text Add to dashboard Cite

Introduction: RBM5 acts as a tumor suppressor gene in lung and breast cancers; however, its role in the pathogenesis of medulloblastoma (MB) remains unclear. We previously identified 4 RBM5 mutations in whole exome sequencing analysis of 40 MB patients. This study examined the role of RBM5 in MB progression. Methods: The expression patterns of RBM5 in tissues of 40 MB patients were analyzed using immunohistochemistry. Associations between RBM5 expression and overall survival (OS) were evaluated using Kaplan-Meier analysis. The RBM5 role in Daoy cells' proliferation, migration, and Wnt/β-catenin signaling was analyzed after RBM5 knockdown and overexpression. Results: The expression level of RBM5 mRNA and protein was significantly lower in MB than that in adjacent normal control tissues, and low RBM5 expression was significantly associated with reduced OS (p = 0.034). RBM5 knockdown induced Daoy and ONS-76 cells proliferation, while RBM5 overexpression repressed cell proliferation and migration in vitro (all p < 0.05). β-Catenin, LEF1, and cyclin D1 mRNA levels were upregulated, while DKK1 expression was downregulated in Daoy cells following RBM5 knockdown. Conclusion: RBM5 may function as a tumor suppressor in MB by regulating Wnt/β-catenin signaling, and its reduced expression is associated with lower OS.

show abstract

Section: Discussionsupporting

confidence: 80%

RBM5 Acts as Tumor Suppressor in Medulloblastoma through Regulating Wnt/β-Catenin Signaling

Wei

et al. 2020

Eur Neurol

View full text Add to dashboard Cite

show abstract

“…The other cell lines present distinct integrin and cadherin expression from U251, which corroborates with the need of the collagen addition for spheroid formation. 50,51 The spheroid size and growth rate of the different cell lines may be explained by analyzing several factors. We have showed in this work that collagen may induce cell proliferation and is a factor that explains the spheroids growth, but it does not explain the difference between cell lines.…”

Section: Resultssupporting

confidence: 50%

Type-I Collagen/Collagenase Modulates the 3D Structure and Behavior of Glioblastoma Spheroid Models

Calori

Alves

Hong

et al. 2022

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Multicellular tumor spheroids have emerged as well-structured, three-dimensional culture models that resemble and mimic the complexity of the dense and hypoxic cancer microenvironment. However, in brain tumor studies, a variety of glioblastoma multiforme (GBM) cell lines only self-assemble into loose cellular aggregates, lacking the properties of actual glioma tumors in humans. In this study, we used type-I collagen as an extracellular matrix component to promote the compaction of GBM aggregates forming tight spheroids to understand how collagen influences the properties of tumors, such as their growth, proliferation, and invasion, and collagenase to promote collagen degradation. The GBM cell lines U87MG, T98G, and A172, as well as the medulloblastoma cell line UW473, were used as standard cell lines that do not spontaneously self-assemble into spheroids, and GBM U251 was used as a self-assembling cell line. According to the findings, all cell lines formed tight spheroids at collagen concentrations higher than 15.0 μg mL–1. Collagen was distributed along the spheroid, similarly to that observed in invasive GBM tumors, and decreased cell migration with no effect on the cellular uptake of small active molecules, as demonstrated by uptake studies using the photosensitizer verteporfin. The enzymatic cleavage of collagen affected spheroid morphology and increased cell migration while maintaining cell viability. Such behaviors are relevant to the physiological models of GBM tumors and are useful for better understanding cell migration and the in vivo infiltration path, drug screening, and kinetics of progression of GBM tumors.

show abstract

“…To date, several studies confirmed the subgroup classification of four cell lines, which Daoy, UW402, and UW473 cells were classified as SHH subgroup with TP53 mutant while ONS‐76 cells were classified as SHH subgroup with TP53 wild‐type. [ 52,53 ] Herein, we found that geniposide treatment notably inhibited human medulloblastoma Daoy, UW402, UW473, and ONS‐76 cell proliferation, but promoted cell apoptosis, which evidenced that geniposide exhibited antitumor effects on medulloblastoma by repressing medulloblastoma cell growth. A previous investigation by Huang et al [ 21 ] reported that geniposide could be used as a chemotherapy sensitizer for doxorubicin‐treated osteosarcoma in vitro and in vivo by inhibiting the efflux function and expression of P‐glycoprotein.…”

Section: Discussionmentioning

confidence: 90%

Retracted: Geniposide exhibits anticancer activity to medulloblastoma cells by downregulating microRNA‐373

2020

J Biochem & Molecular Tox

View full text Add to dashboard Cite

Background: Medulloblastoma is a common tumor originates from central nervous system in children with metastatic potential. Geniposide is the major active ingredient separated from the fruit of Gardenia jasminoides Ellis. Herein, we tested the possible anticancer activity of geniposide on human medulloblastoma cells, as well as the potential underlying molecular mechanisms.Methods: Firstly, followed by geniposide incubation, cell viability, proliferation, apoptosis, migration, and invasion of medulloblastoma Daoy cells, along with microRNA-373 (miR-373) expression were tested, respectively. Then, the influences of miR-373 overexpression in the reduction of medulloblastoma cell proliferation, migration, and invasion and the elevation of apoptosis, triggered by geniposide treatment, were reinvestigated. Finally, the Ras/Raf/MEK/ERK pathway activity was analyzed. Results: Geniposide treatment inhibited medulloblastoma cell viability, proliferation, migration, and invasion, but promoted cell apoptosis. Surprisingly, miR-373 expression in medulloblastoma cells was obviously downregulated by geniposide treatment. miR-373 overexpression reversed the effects of geniposide on Daoy cells. Furthermore, geniposide hindered the Ras/Raf/MEK/ERK pathway by downregulating miR-373 expression. Conclusion: Geniposide exhibited anticancer activity on human medulloblastoma cells and blocked Ras/Raf/MEK/ERK pathway by downregulating miR-373 expression. K E Y W O R D S cell metastasis, cell proliferation, geniposide, medulloblastoma, microRNA-373, Ras/Raf/MEK/ ERK pathway

show abstract

Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines

Cited by 8 publications

References 51 publications

RBM5 Acts as Tumor Suppressor in Medulloblastoma through Regulating Wnt/β-Catenin Signaling

RBM5 Acts as Tumor Suppressor in Medulloblastoma through Regulating Wnt/β-Catenin Signaling

Type-I Collagen/Collagenase Modulates the 3D Structure and Behavior of Glioblastoma Spheroid Models

Retracted: Geniposide exhibits anticancer activity to medulloblastoma cells by downregulating microRNA‐373

Contact Info

Product

Resources

About