Biological Control Against Four-Stored Product Beetles Pests by Using Cytoplasmic Polyhedrosis Virus (Cypovirus1)

Abstract

The rice weevils, tobacco beetle, drug beetle, and Darkling beetle were collected from different locations in Mecca regions, KSA. The four different larvae attack stored products cause economic losses by incredible damage and post-harvest. This investigation was Carrie out to identify characters these insects include. In addition, Cypovirus1 was isolated and collected from living and dead stem and cob borer larvae and pupae from Maize in farmers' fields at Mecca regions, KSA. Samples have chronic diseases were tested by indirect ELISA (The enzyme-linked immunosorbent assay) and confirmed by TEM (Transmission electron microscopy) showing inclusion bodies demonstrated that the occlusion bodies were of irregular shape and ranged from 2.2 to 4.9 µm in diameter. On the other hand, we are studying the effects of different concentrations of a new Biocomponent of Cypovirus1 infection on the rice weevil adults, tobacco beetle, bread beetle, and darkling beetle larvae study their impact on the rates and severity of the infection. Also, the SDS-PAGE (Sodium dodecyl sulfatepolyacrylamide gel electrophoresis) test for showing various distinguishable sole bands in infected adults comparing with non-infected.