Biological control of Culex (Culex) saltanensis Dyar, (Diptera, Culicidae) through Bacillus thuringiensis israelensis in laboratory and field conditions

Zequi, João Antonio Cyrino; Lopes, José

doi:10.1590/s0101-81752007000100020

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…The utilisation of these products, however, has been limited because they are non-specific, pollute the environment, and their target insects have high rates of resistance. This situation has led to the development of biological insecticides using bacteria, fungi, viruses, and other alternative organisms (Consoli et al, 1997;Regis et al, 2001;Arantes et al, 2002;Tauil, 2002;Zequi and Lopes, 2007;Russell and Kay, 2008). The emphasis of this study is on the use of entomopathogenic bacteria of the genus Bacillus because of its high degree of specificity and environmental safety, thus particularly suitable and environmentally friendly for use in the control of insect pests.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a new formulation of Bacillus thuringiensis israelensis

2010

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The aim of this study was to determine the potency (ITU) and efficacy of a liquid formulation of Bacillus thuringiensis israelensis developed by the State University of Londrina named BioUel, against early fourth instar larvae of Aedes aegypti and Culex quinquefasciatus. The ITU/mg of BioUel was 960, the LC50 was of 0.271 (± 0.39) ppm, and the LC95 was 0.634 (± 0.099) ppm, in larvae of C. quinquefasciatus. In A. aegypti larvae, LC50 was 0.332 (± 0.042) ppm and LC95 was 0.694 (± 0.073) ppm. The ITU level of BioUel and its control results were similar to most commercial products tested. Stability was of approximately 90 days, which allows for local production.

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Section: Introductionmentioning

confidence: 99%

Evaluation of a new formulation of Bacillus thuringiensis israelensis

2010

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“…Becker et al (1992), que encontraram diferença na eficácia de B. thuringiensis israelensis especialmente entre 5º e 8ºC em 2º e 4º instares de Aedes vexans (Meigen 1830), provavelmente pela redução da filtração das larvas em baixas temperaturas, sugerindo que se deve aplicar grande quantidade de produto abaixo de 8ºC para esta espécie. Culex saltanensis, exposto a B. thuringiensis israelensis na temperatura de 12 ± 1ºC, e fotoperíodo 14L:10E, teve sua suscetibilidade diminuída em 1,5 vezes em relação a CL 50 , quando comparado com a temperatura ambiente (Zequi & lopes 2007).…”

Section: Resultsunclassified

Controle de Aedes (Stegomyia) aegypti (Linnaeus, 1762) e Culex (Culex) quinquefasciatus Say, 1823 através de formulados contendo Bacillus thuringiensis israelensis em temperaturas controladas

2011

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Aedes (Stegomyia) aegypti (Linnaeus) e Culex (Culex) quinquefasciatus Say são importantes vetores de patógenos em áreas urbanas. Este trabalho teve como objetivo avaliar diferentes formulados comerciais contendo Bacillus thuringiensis israelensis de Barjac, no controle de Ae. aegypti e Cx. quinquefasciatus, verificando sua eficiência perante a potencia declarada. Testou-se os produtos Aquabac® XT 1200 UTI/mg, Teknar® 3000AAU/mg e Vectobac® AS 1200 UTI/mg, formulados líquidos, e os sólidos Vectobac® WDG 3000 UTI/mg, Vectobac® T 2200 UTI/mg e o formulado experimental Biouel 500 UTI/mg. Todos os produtos foram testados a temperatura de 25±2°C e os líquidos também a 15±2°C, 35±2°C e temperatura ambiente (25,37 a 28,73ºC). Os testes foram realizados utilizando-se 25 larvas no início do 4º estádio, em 150 mL de água destilada, com leitura de mortalidade 24 horas após exposição. Resultados foram analisados através de Probit calculando-se a CL50. A 25±2°C, os produtos Vectobac WDG e Vectobac T foram os mais eficientes para Ae. aegypti, com CL50 respectivamente de 0,10 (0,08 – 0,11) mg/L e 0,10 (0,09 – 0,11 mg/L). Para Cx. quinquefasciatus, os mais eficientes foram Vectobac WDG, Vectobac AS, Biouel e Vectobac T. Tomando como referência a potencia declarada dos produtos, o Biouel teve, proporcionalmente, melhor desempenho para as duas espécies. Nas temperaturas de 15±2°C, 35±2°C e na temperatura ambiente (25,37 a 28,37°C), Vectobac AS foi o mais eficiente para as duas espécies de Culicidae testadas. Na temperatura ambiente a 35±2°C, necessitou-se de menor concentração dos produtos para controle de Cx. quinquefasciatus em relação à Ae. aegypti. Control of Aedes (Stegomyia) aegypti and Culex (Culex) quinquefasciatus Through Formulated Containing Bacillus thuringiensis israelensis at Controlled Temperatures Abstract. Aedes (Stegomyia) aegypti (Linnaeus) and Culex (Culex) quinquefasciatus Say are important pathogen vectors in urban environments. This study was designed to evaluate commercial formulations containing Bacillus thuringiensis israelensis de Barjac for the control of Ae. aegypti and Cx. quinquefasciatus and to assess their efficiency as compared to manufacturers´ claims. The tested products were the liquid formulation of Aquabac® XT 1200 ITU/mg, Teknar® 3000AAU/mg, and Vectobac® AS 1200 ITU/mg, and the solid products Vectobac® WDG 3000 ITU/mg, Vectobac® Tablet 2200 ITU/mg, and the trial formulation of Biouel 500 ITU/mg. All products were tested at 25±2°C temperature and the liquid formulations were also tested at 15±2°C, 35±2°C, and at room temperature (25.37 to 28.73ºC). The experiments were conducted with 25 larvae at the early 4th stage, in 150 mL of distilled water; the dead larvae were counted 24 hours after product application. Results were analyzed using Probit to calculate CL50. The 25±2°C temperature, Vectobac WDG, and Vectobac Tablet were the most efficient in controlling Ae. aegypti, with CL50 of 0.10 (0.08 – 0.11) mg/L and 0.10 (0.09 – 0.11 mg/L), respectively. The most efficient products for Cx. quinquefasciatus were Vectobac WDG, Vectobac AS, Biouel, and Vectobac T. When the potency claimed by manufacturers was compared to our laboratory results, Biouel had the best performance for both species. Vectobac AS was the most efficient for both species of Culicidae tested at 15±2°C, 35±2°C and at room temperature (25.37 to 28.37°C). Lower product concentrations were required at 35±2°C room temperature to control Cx. quinquefasciatus than for Ae. aegypti.

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“…The totality of individuals inhabiting artificial breeding sites and the majority being found in the urban environment reveal the high adaptability of this species to modified environments, in accordance with the synanthropic index. Despite this, this species can also be found colonizing natural breeding grounds (Zequi & Lopes, 2007; Zequi & Lopes, 2012).…”

Section: Discussionmentioning

confidence: 99%

Synanthropic Culicidae (Diptera) in three fragments of Atlantic Forest in northern Parana, Brazil

Grossi,

da Silva,

Novaes

et al. 2023

Preprint

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Synanthropic Culicidae were sampled using natural and artificial traps in three forest fragments (Daher Wood, Botanical Garden, and Mata dos Godoy State Park) in Londrina, Paraná, Brazil. To collect Culicidae, six traps were distributed in two separate transects of 70 m each, parallel, with a distance between them of 30 m. Three traps were natural (bamboo) and the other three were artificial (tires). In total, we collected 15,048 specimens distributed in eight species. The peri-urban fragments showed the highest richness. Tires were preferred as breeding sites. The seasons of highest and lowest sampling were summer and winter. The Daher Wood and Botanical Garden showed high similarity, and the Godoy Wood, an intact environment, showed the greatest dominance. The mosquitoes demonstrated varying degrees of synanthropy. Correlations were positive between temperature (0.53) and precipitation (0.40) with Culicid abundance. The Principal Component Analysis indicated that tires were most influenced by temperature, relative humidity, and bamboos by Total Dissolved Solids. Four of the collected species showed potential to be vectors of etiological agents. Abiotic factors directly influence the biology of mosquitoes, which is reflected in higher diversity in warmer and rainy periods. Urban and peri-urban areas showed more synanthropic mosquitoes due to their preference for modified sites. Vector species in these areas are of concern because they can modify disease cycles, and for this reason, it is essential to monitor these areas.

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Biological control of Culex (Culex) saltanensis Dyar, (Diptera, Culicidae) through Bacillus thuringiensis israelensis in laboratory and field conditions

Cited by 5 publications

References 15 publications

Evaluation of a new formulation of Bacillus thuringiensis israelensis

Evaluation of a new formulation of Bacillus thuringiensis israelensis

Controle de Aedes (Stegomyia) aegypti (Linnaeus, 1762) e Culex (Culex) quinquefasciatus Say, 1823 através de formulados contendo Bacillus thuringiensis israelensis em temperaturas controladas

Synanthropic Culicidae (Diptera) in three fragments of Atlantic Forest in northern Parana, Brazil

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