Biological control of engorged female Haemaphysalis qinghaiensis (Acari: Ixodidae) ticks with different Chinese isolates of Beauveria bassiana

Ren, Qiaoyun; Sun, Ming; Guan, Guiquan; Li, Youquan; Liu, Zhijie; Liu, Aihong; Ma, Miling; Niu, Qingli; Liu, Junlong; Yin, Hong; Luo, Jianxun

doi:10.1007/s00436-011-2346-z

Cited by 7 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All tested isolates showed pathogenicity toward R. microplus unfed larvae. Other isolates of I. farinosa, I. fumosorosea, and P. lilacinum should be tested against ticks once different levels of virulence have been detected among isolates of the same species of entomopathogenic fungus to many arthropod species, including ticks (Alves 1998;Lomer et al 2001;Fernandes et al 2006Fernandes et al , 2009Fernandes et al , 2010Pirali-Kheirabadi et al 2007;Ren et al 2011). As far as we know, this is the first report of virulence of I. farinosa, I. fumosorosea, and P. lilacinum to R. microplus ticks.…”

Section: Discussionmentioning

confidence: 84%

“…Many pathogenic fungi are known to be associated with ticks, including Beauveria bassiana, Isaria fumosorosea, I. farinosa, and Lecanicillium spp. Many studies have experimentally confirmed the pathogenicity of fungi, such as B. bassiana and Metarhizium anisopliae, to different tick developmental stages (Fernandes et al 2006;Pirali-Kheirabadi et al 2007;Fernandes and Bittencourt 2008;Ren et al 2011). Fungal pesticides have been proved to be efficient against a large variety of pests.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Virulence of Isaria sp. and Purpureocillium lilacinum to Rhipicephalus microplus tick under laboratory conditions

et al. 2012

View full text Add to dashboard Cite

Rhipicephalus microplus (Canestrini) is an ectoparasite accountable for great economic losses. The use of entomopathogenic fungi to control arthropods has shown promising responses. The present study evaluated the virulence of Isaria farinosa (Holmsk.) Fr., Isaria fumosorosea (Wize) Brown and Smith, and Purpureocillium lilacinum (=Paecilomyces lilacinus) (Thom.) Samson to engorged females, eggs, and larvae of R. microplus. There were four treatment groups (10(5), 10(6), 10(7), and 10(8) conidia ml(-1)) and the control group (water and Tween 80, 0.1% v/v). The treatment was based on immersion of the specimen in 1 ml of the suspension or control solution. The study observed changes in egg viability and larval mortality after treatment. The results showed that I. farinosa, P. lilacinum, and I. fumosorosea caused alterations in the biological parameters of R. microplus ticks. I. fumosorosea presented the greatest potential to control R. microplus engorged females in vitro, causing a 49% decrease in nutritional index. All fungal isolates presented significant reduction in the egg production index. I. farinosa reduced the hatching percentage if the eggs were treated with the two highest conidial concentrations. All conidial concentrations of I. fumosorosea were able to reduce the hatching percentage significantly. All tested isolates showed pathogenicity toward unfed R. microplus larvae. As far as we know, this is the first study reporting the effect in vitro of I. farinosa, I. fumosorosea, and P. lilacinum to different developmental stages of R. microplus ticks.

show abstract

Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Virulence of Isaria sp. and Purpureocillium lilacinum to Rhipicephalus microplus tick under laboratory conditions

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Therefore, for sustainable control of ticks, adoption of Integrated Pest Management involving different eco-friendly control methods such as immunological, biological, exploration of host resistance to ticks, herbal, etc. de la Fuente et al 2007;Ren et al 2011;Pirali-Kheirabadi et al 2007;Monteiro et al 2010;Rees 2004;Wambura et al 1998;MartinezVelazquez et al 2011;Ghosh et al 2011) is the only option.…”

Section: Introductionmentioning

confidence: 95%

Efficacy of rBm86 against Rhipicephalus (Boophilus) microplus (IVRI-I line) and Hyalomma anatolicum anatolicum (IVRI-II line) infestations on bovine calves

et al. 2012

View full text Add to dashboard Cite

With an aim to evaluate the protective potentiality of rBm86 against Rhipicephalus (Boophilus) microplus Indian Veterinary Research Institute (IVRI)-I line and Hyalomma anatolicum anatolicum IVRI-II line infestations on crossbred (Bos indicus × Bos taurus) calves, 20 animals of 3 months of age were randomly divided in to four equal groups and maintained in tick-proof conditions. Animals of groups 1 and 2 were immunized with 2 ml of rBm86 (100 μg)-based vaccine (procured from Revetmex S.A. de C.V, Mexico City, Mexico) thrice at 30 days interval. Animals of groups 3 and 4 were kept as negative control and inoculated with PBS only. Each animal of group 1 and 3 was challenged with 7-day-old 50 unfed adults of H. anatolicum anatolicum (1:1, male and female), and each animal of groups 2 and 4 was challenged with 6-8-day-old R. (B.) microplus larvae obtained from 50 mg of eggs, on 17th day of the last immunization. The efficacy of rBm86 against tick infestations was determined as percentage reduction in number of adults dropped (DT%), engorged body weight (DR%), egg masses (DO%), and immunogen efficacy (E%). The calculated data were 11.8, 10.8, 15.0, and 25.1 %, respectively, for DT, DR, DO, and E% against H. anatolicum anatolicum infestation, while in the case of R. (B.) microplus infestation, the corresponding data were 6.4, 11.24, 40.7, and 44.5 %, respectively. The results indicated partial effectiveness of rBm86 antigen(s) in imparting protection against homologous and heterologous challenge infestations of Indian ticks. The results indicated identification of more effective antigen(s) for the development of vaccine against economically important tick species in India.

show abstract

Neutral lipid composition changes in the fat bodies of engorged females Rhipicephalus microplus ticks in response to fungal infections

et al. 2012

View full text Add to dashboard Cite

The tick's fat body plays an essential role in energy storage and utilization. This study aimed to analyze the fat body neutral lipid composition in Rhipicephalus microplus engorged females. In the first study (physiological profile of untreated ticks), the lipid analysis took place over the course of 4 days; the engorged females were incubated at optimal conditions and their fat bodies were dissected daily. Fat body lipid analysis after fungal infection with Metarhizium anisopliae sensu lato (s.l.) or Beauveria bassiana s.l. was performed with four groups: one without any treatment, one that was inoculated with a solution of 0.1 % Tween 80 in water, and two groups that were inoculated with M. anisopliae or B. bassiana conidial suspensions. The fat bodies were dissected 24 and 48 h after infection. Lipid analysis was conducted by thin-layer chromatography on a silica plate. The results of the physiological profile showed that the amounts of triacylglycerol (TAG) and free cholesterol (CHO) decreased with time, whereas cholesterol ester (CHOE) increased on the second and fourth days. Following M. anisopliae or B. bassiana infection, there was an increase in the amount of CHO after 24 h, whereas the other lipid classes were not altered. M. anisopliae caused an increase in CHOE and TAG and a reduction in CHO at 48 h after infection; however, B. bassiana infection did not cause significant alterations in the concentrations of these lipids. M. anisopliae and B. bassiana infection changed the fat body metabolism of engorged female R. microplus ticks. This study provides the first report of changes in the neutral lipid composition of the R. microplus fat body.

show abstract

Biological control of engorged female Haemaphysalis qinghaiensis (Acari: Ixodidae) ticks with different Chinese isolates of Beauveria bassiana

Cited by 7 publications

References 31 publications

Virulence of Isaria sp. and Purpureocillium lilacinum to Rhipicephalus microplus tick under laboratory conditions

Virulence of Isaria sp. and Purpureocillium lilacinum to Rhipicephalus microplus tick under laboratory conditions

Efficacy of rBm86 against Rhipicephalus (Boophilus) microplus (IVRI-I line) and Hyalomma anatolicum anatolicum (IVRI-II line) infestations on bovine calves

Neutral lipid composition changes in the fat bodies of engorged females Rhipicephalus microplus ticks in response to fungal infections

Contact Info

Product

Resources

About