Biological control of foot rot (Phytophthora capsici Leonian) disease in black pepper (Piper nigrum L.) with rhizospheric microorganisms

Nysanth, N.S.; Divya, S.; Nair, Chitra B.; Anju, A.B.; Praveena, R.; Anith, K. N.

doi:10.1016/j.rhisph.2022.100578

Cited by 13 publications

(4 citation statements)

References 42 publications

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“…Further, the study emphasizes the importance of using BCAs for disease control, as antagonistic organisms have proven effective in not only combating plant pathogens but also enhancing agricultural crop production. Various species of antagonistic fungi, such as T. harzianum, Gliocladium, Paecilomyces, Penicillium, and Verticillium, have demonstrated satisfactory control of destructive plant diseases (Panth et al, 2020;Nysanth et al, 2022;Huilgol et al, 2022). The present experiment indicates the effectiveness of specific BCAs in inhibiting F. oxysporum growth, increasing root and shoot growth, and reducing plant mortality.…”

Section: Discussionmentioning

confidence: 52%

Comparative study of bio-control agents against fusarium wilt in okra caused by Fusarium oxysporum pv. vasinfectum

Fatima,

Amjad Ali,

Jabran

2023

Phytopathogenomics and Disease Control

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Okra (Abelmoschus esculentus) is a widely cultivated vegetable crop that is susceptible to various pathogens, including Fusarium oxysporum, which causes severe disease of wilt. Chemical control using fungicides currently poses risks to human and animal health. To find ecofriendly method, the present study aimed to compare the effectiveness of different bio-control agents (BCAs) against Fusarium wilt in Okra for eco-safe management. Okra seeds were taken from Vegetable Research Institute, Ayub Agricultural Research Institute. Disease samples were collected, and the pathogen was isolated, purified, and multiplied by using potato dextrose agar media. Pure culture was used for the isolation of DNA and amplification of the ITS region was accomplished by using ITS-1 and ITS-4 primers. The fragments were visualized by running it on 1% agarose gel. The PCR product of F. oxysporum pv. vasinfectum was successfully confirmed based on size of fragment by comparing with 1 kb marker. It was verified that the selected primer pair specifically amplified the anticipated 18S rDNA fragment, measuring 500 base pairs in length. Moreover, in-vitro tests revealed that BCAs like Bacillus spp. inhibited Fusarium fungus by 41.2%, while Trichoderma inhibited it by 38.5%. Control groups exhibited no inhibition of pathogen growth. Taken together, this effort has important implications for the implementation of biological management strategies in Okra field to mitigate the impact of Fusarium wilt disease.

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Section: Discussionmentioning

confidence: 52%

Comparative study of bio-control agents against fusarium wilt in okra caused by Fusarium oxysporum pv. vasinfectum

Fatima,

Amjad Ali,

Jabran

2023

Phytopathogenomics and Disease Control

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“…Black pepper is affected by various pathogens [11]. Of all these, quick wilt disease is the most damaging one [12]. Studies reported that quick wilt is a severe disease in different pepper growing countries.…”

Section: Quick Wiltmentioning

confidence: 99%

ROLE OF CRISPR/Cas IN CONTROLLING BIOTIC STRESS IN BLACKPEPPER

K S

2023

JASR

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Black pepper (Piper nigrum L.), the king of spices is one of the most important traditional spices cultivated all over world. Over the past 15 years, there has been a noticeable decline in crop production and area due to biotic and abiotic stress. Despite the efforts made to develop and select a number of black pepper varieties with high yield potential and disease tolerance, the situation has not improved in a decade. Quick wilt caused by Phytopthora capsici, one of the major soil-borne fungi can destroy black pepper crops and cause heavy loss in the plantations. All plant parts are vulnerable to infection, which results in significant decrease in gene expression, thereby inducing heavy mortality rate. Different resistant varieties are raised based on different breeding programs to control the disease and helps in maintaining black pepper production. Such labor-intensive, unfocused breeding initiatives that take so much time and effort cannot keep up with the needs for higher crop production. Currently, a novel gene editing technique known as the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas technology, has succeeded in enhancing crop quality that increase yield, quality as well as to improve resistance to biotic and abiotic stresses. The main objective of this review is to identify the role of CRISPR/Cas technology in controlling the quick wilt fungi by genome editing. Recent improvements in CRISPR/Cas genome editing allow for effective targeted modification in the majority of crops, which promises to hasten crop development, especially in commercially important crop like black pepper.

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“…Biocontrol thus becomes vital in the organic production of black pepper. 3 In high-value crops, microorganisms can be employed successfully as a secure alternative to traditional plant health management techniques.…”

Section: Introductionmentioning

confidence: 99%

Bacterization with Endospore-forming Bacillus spp. Promotes Plant Growth and Suppresses Foot Rot Disease in Black Pepper (Piper nigrum L.) in the Nursery

Anju¹,

Natarajan²,

Preetha³

et al. 2023

J. Pure Appl. Microbiol.

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Foot rot disease (Phytophthora capsici) causes severe economic losses to cultivators of black pepper. Fungicides used for managing the disease adversely affect the export potential of the product due to their residual toxicity. Endospore-forming bacterial strains, Bacillus amyloliquefaciens VLY24, Bacillus pumilus VLY17, and Bacillus velezensis PCSE10 were evaluated for growth promotion and disease suppression in black pepper nursery at two locations in Kerala, India. All the isolates could suppress Phytophthora capsici under in vitro conditions. Spraying detached leaves with a cell suspension of the bacterial isolates could delay the development and progression of lesion. In the in vivo trials, bacterized cuttings had a better establishment in the nursery when compared to uninoculated control. Plant growth parameters like the number of leaves, roots, and shoots were more in bacterized cuttings. Cuttings treated with Bacillus pumilus VLY17 when challenge inoculated with the pathogen, showed 84.74 percent reduction in the size of lesions on the leaves, and showed the least disease index (0.27) compared to pathogen inoculated control. It is proposed that endospore forming Bacillus spp. with anti-oomycetic activity could be potential biocontrol agents against foot rot disease of black pepper.

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Biological control of foot rot (Phytophthora capsici Leonian) disease in black pepper (Piper nigrum L.) with rhizospheric microorganisms

Cited by 13 publications

References 42 publications

Comparative study of bio-control agents against fusarium wilt in okra caused by Fusarium oxysporum pv. vasinfectum

Comparative study of bio-control agents against fusarium wilt in okra caused by Fusarium oxysporum pv. vasinfectum

ROLE OF CRISPR/Cas IN CONTROLLING BIOTIC STRESS IN BLACKPEPPER

Bacterization with Endospore-forming Bacillus spp. Promotes Plant Growth and Suppresses Foot Rot Disease in Black Pepper (Piper nigrum L.) in the Nursery

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