Biological control of fusarium head blight of wheat withClonostachys roseastrain ACM941

Abstract

Fusarium head blight (FHB), caused by Gibberella zeae, is a devastating disease of wheat. A strain of Clonostachys rosea, ACM941 (American Type Culture Collection ATCC 74447), was evaluated for antibiosis against G. zeae in vitro and for control of FHB under greenhouse and field conditions in comparison to the registered fungicide Folicur (tebuconazole). Strain ACM941 reduced mycelial growth of the pathogen by 52.6% in dual-culture after 6 days and completely suppressed spore germination for 6 h when cocultured with a macroconidial suspension of G. zeae. Strain ACM941 reduced G. zeae perithecial production by more than 99% in a leaf disk assay, 60%-77% on infected corn kernels, and 32%-57% on spikelet debris in the field. These effects were significant (P < 0.05) and not statistically different from those produced by tebuconazole. When strain ACM941 was sprayed onto wheat heads 2 days prior to inoculation with G. zeae, it significantly reduced infected spikelets (IS) by 64% and Fusarium-damaged kernels (FDK) by 65% in greenhouse experiments. Under simulated disease epidemic conditions during 2005-2007, strain ACM941 reduced the FHB index by 58%, IS by 46%, FDK by 49%, and deoxynivalenol (DON) in kernels by 21%. These effects were significant but lesser in magnitude than those achieved by tebuconazole, which reduced FHB index by 97%, IS by 82%, FDK by 73%, and DON by 62%. Results from this research suggest that strain ACM941 of C. rosea is a promising biocontrol agent against G. zeae and may be used as a control measure in an integrated FHB management program. Key words: biological control, Gibberella zeae, fusarium head blight, Clonostachys rosea, wheat. Xue et al.: fusarium head blight / biological control / Clonostachys rosea 179 Résumé : La fusariose de l'épi (FHB), causée par Gibberella zeae, est une maladie dévastatrice qui s'attaque au blé. La souche ACM941 (ATCC 74447) de Clonostachys rosea a été évaluée, in vitro, sur le plan de l'antibiose pour lutter contre G. zeae et, en serre et au champ, pour lutter contre la FHB afin d'en comparer les effets à ceux du traitement fongicide homologué Folicur (tébuconazole). Au bout de six jours, lors de tests en culture duale, la souche ACM941 a réduit de 52,6 % la croissance mycélienne de l'agent pathogène et en a entièrement inhibé la germination des spores pendant six heures lorsqu'on l'a fait croître en présence d'une suspension macroconidiale de G. zeae. Lors d'un test biologique sur disque foliaire, la souche ACM941 a réduit la production périthéciale de plus de 99 %, de 60 % à 77 % lors d'un test sur des grains de maïs et de 32 % à 57 %, au champ, lors d'un test sur des débris d'épillets. Ces résultats étaient significatifs (P < 0,05) et semblables statistiquement à ceux obtenus avec le tébuconazole. Lorsque, au cours d'expériences menées en serres, la souche ACM941 a été pulvérisée sur des épis de blé deux jours avant de les inoculer avec G. zeae, les taux d'infection des épillets (IE) et les dommages subits par les grains affectés par Fusarium (DGF) ont été s...

Microbiota in Wheat Roots, Rhizosphere and Soil in Crops Grown in Organic and Other Production Systems

“…; Hue et al. ). Many others would be secondary invaders or participants in synergistic enhancement of pathogenicity.…”

“…The fungi detected and quantified in each system were, where possible, classified as potential pathogens (Nguyen et al 1973;Hosford 1975;Desjardins et al 1993;Stiles and Murray 1996;Amarlou et al 2010;Bockus et al 2010) or potential antagonists of wheat pathogens (Mangan 1967;Fokkema and van der Meulen 1976;McGee et al 1991;Harman and Kubicek 1998;Xue 2002;Aggarwal et al 2004;Hue et al 2009). Many others would be secondary invaders or participants in synergistic enhancement of pathogenicity.…”

The mycoparasitic fungus Clonostachys rosea responds with both common and specific gene expression during interspecific interactions with fungal prey

“…nov. (Schroers, Samuels, Seifert, & Gams, 1999) is an ascomycete fungus that is reported to control diseases caused by a wide range of plant pathogenic fungi, including Alternaria spp. (Jensen, Knudsen, Madsen, & Jensen, 2004), Bipolaris sorokiniana (Jensen, Knudsen, & Jensen, 2002), Botrytis cinerea , Fusarium culmorum (Jensen, Knudsen, & Jensen, 2000), Fusarium graminearum (Xue et al, 2009) and Sclerotinia sclerotiorum (Rodriguez, Cabrera, Gozzo, Eberlin, & Godeas, 2011). Several biocontrol mechanisms are reported in C. rosea, including direct parasitism of pathogenic fungi (Li, Huang, Kokko, & Acharya, 2002;, antibiosis (Pachenari & Dix, 1980;Rodriguez et al, 2011), production of fungal cell wall degrading enzymes (Chatterton & Punja, 2009;Mamarabadi, Jensen, & Lubeck, 2008), induction of plant defence reactions (Lahlali & Peng, 2014;Roberti et al, 2008) and plant growth promotion (Roberti et al, 2008).…”

Integration of proteome and transcriptome data reveals the mechanism involved in controlling of Fusarium graminearum by Saccharomyces cerevisiae

“…The metabolic and reproductive needs of the bacteria themselves slow down electron transfer and energy metabolism to achieve the inhibitory effect. 29 Hue et al 30 isolated a strain of Clonostachys rosea which was found to have a strong inhibitory effect on the growth and spore germination of F. graminearum. Our experimental results showed that S. cerevisiae Y-912 had a strong inhibitory effect on the growth of F. graminearum Fg1 and could control its germination rate and germ tube length.…”