Biological control of Fusarium wilt of tomato with hypovirulent binucleate Rhizoctonia in greenhouse conditions

Muslim, A.; Horinouchi, Hayato; Hyakumachi, Mitsuro

doi:10.1007/s10267-002-0084-x

Cited by 36 publications

(26 citation statements)

References 37 publications

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“…Intensive use of chemical fungicides has led to fungicide resistance and increased soil contamination; furthermore, these compounds may be toxic to microbial communities [2, 3]. Biocontrol with beneficial microorganisms has been shown to be an environmentally sound option to increase crop yields and prevent outbreaks of pathogenic bacteria [4, 5]. Plant growth-promoting rhizobacteria (PGPR), soil bacteria that colonize plant roots, interact with plant roots and can influence plant growth by scavenging reactive oxygen species, hydrogen peroxide, and volatile compounds [6, 7].…”

Section: Introductionmentioning

confidence: 99%

Bacillus methylotrophicus Strain NKG-1, Isolated from Changbai Mountain, China, Has Potential Applications as a Biofertilizer or Biocontrol Agent

Liu

Nwet

et al. 2016

PLoS ONE

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Chemical pesticides are widely used in agriculture, which endangers both environmental health and food safety. Biocontrol is an environmentally-friendly and cost-effective green technique in environmental protection and agricultural production; it generally uses selected bioresources, including beneficial microorganisms. We isolated a novel bacterial strain (NKG-1) from the rare dormant volcanic soils of Changbai Mountain in China’s Jilin Province. The strain was identified as Bacillus methylotrophicus using morphological, biochemical, physiological, and phylogenetic 16S rDNA sequencing data. This strain was able to suppress mycelial growth and conidial germination of numerous plant pathogenic fungi on solid media. A greenhouse experiment showed that application of NKG-1 fermentation broth prior to inoculation of Botrytis cinerea, the cause of gray tomato mold, inhibited growth of the mold by 60%. Furthermore, application of a 100× dilution of NKG-1 fermentation broth to tomato seedlings yielded a significant increase in seedling fresh weight (27.4%), seedling length (12.5%), and root length (57.7%) compared to the control. When the same dosage was applied in the field, we observed increases in tomato plant height (14.7%), stem diameter (12.7%), crown width (16.3%), and maximum fruit diameter (11.5%). These results suggest that NKG-1 has potential commercial application as a biofertilizer or biocontrol agent.

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Section: Introductionmentioning

confidence: 99%

Bacillus methylotrophicus Strain NKG-1, Isolated from Changbai Mountain, China, Has Potential Applications as a Biofertilizer or Biocontrol Agent

Liu

Nwet

et al. 2016

PLoS ONE

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“…Various bioassays for screening biocontrol agents use soil systems [9,11,24], and other bioassays for induced resistance in tomato plants have been reported, such as split root, benomyl, cutting, and layering [25]. However, these systems, like most other biocontrol assay, often require more than one month to complete.…”

Section: Discussionmentioning

confidence: 99%

“…Hypovirulent Binucleate Rhizoctonia (HBNR) were investigated as effective biocontrol agents for a number of important diseases caused by Rhizoctonia solani [7] and Phytium [8]. Our previous research showed that HBNR effectively controls Fusarium wilt of tomato [9], Fusarium wilt of spinach [10], and Fusarium crown and root rot of tomato [11]. These studies indicated that one of the mechanisms of biocontrol of fusarium diseases with HBNR isolates might be induced resistance.…”

Section: Introductionmentioning

confidence: 99%

A Rapid Bioassay to Evaluate Efficacy of Hypovirulent Binucleate Rhizoctonia in Reducing Fusarium Crown and Root Rot of Tomato

Muslim¹,

Hyakumachi²,

Kageyama³

et al. 2019

TOASJ

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Background: Fusarium Oxysporum f.sp. Radicis-Lycopersici (FORL) caused Fusarium Crown and Root Rot of tomato (FCRR), it’s a serious constraint on tomato production and contributing to yield losses. Aims/Method: Using a rapid bioassay, Hypovirulent Binucleate Rhizoctonia (HBNR) was tested for their ability to reduce Fusarium Crown and Root Rot (FCRR) of tomato, caused by Fusarium oxysporum f.sp. radicis lycopersici (FORL). Roots of tomato seedlings growing on 2% water agar in plastic boxes were inoculated with living or dead mycelial disks of HBNR. After 24 h, the pathogen was applied at 0, 3, 6, and 9 cm away from the position of the HBNR. Results: When living HBNR was used, the treatments provided significant protection to tomato seedlings from FCRR infection at all distances tested. Tomato plants pre-inoculated with living HBNR at different times (12 h and 24 h before inoculation with the pathogen) and challenged with FORL showed significant reduction of FCRR lesion development. A significant reduction was still observed even when HBNR was inoculated simultaneously with or 12 h after inoculation of a pathogen. Seedlings treated with dead HBNR and culture filtrates also showed significantly reduced FCRR lesion development. When living HBNR were enveloped by a polycarbonate membrane filter, a significant reduction of FCRR lesion development was still observed. Conclusion: In all experiments, reduction of FCRR lesion development in seedlings treated with HBNR tended to decrease with longer distance from the inoculation point of FORL and HBNR. We developed a simple, rapid, and miniaturized bioassay for evaluating the efficacy of HBNR against FORL. The bioassays require only 12 - 18 days, which is at least 12 days less than the soil system employed by previous researchers.

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“…Masing-masing biakan diinkubasi selama 10-14 hari pada suhu ruangan. Biakan digoyang setiap hari supaya kolonisasi cendawan pada subsrat merata, kemudian substrat yang terkolonisasi oleh masingmasing cendawan dikeringanginkan selama 7 hari dan disimpan pada suhu 4 °C sebelum digunakan (Muslim et al 2003).…”

Section: Bahan Dan Metode Inokulum Fusarium Dan R Solaniunclassified

Fusarium Nonpatogen sebagai Agens Hayati Penyakit Rebah Kecambah pada Tanaman Terung

Muslim

2015

jfi

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Rhizoctonia solani merupakan patogen tular tanah yang sangat penting yang dapat menyebabkan rebah kecambah, busuk batang, dan busuk akar. Penelitian ini bertujuan menentukan kemampuan isolat Fusarium nonpatogen yang diaplikasikan pada awal pembibitan dalam mengendalikan penyakit rebah kecambah yang disebabkan R. solani pada tanaman terung. Perlakuan Fusarium nonpatogen memberikan pengaruh nyata dan sangat efektif dalam menghambat keparahan penyakit rebah kecambah dengan persentase penekanan keparahan penyakit berkisar antara 59.02 dan 96.72%. Perlakuan Fusarium nonpatogen pada pembibitan juga mampu meningkatkan tinggi bibit dan bobot basah berturut-turut berkisar 40.00-60.98%, dan 21.80-66.53%. Hasil penelitian menunjukkan bahwa Fusarium nonpatogen merupakan agens pengendalian biologi yang potensial untuk menanggulangi penyakit rebah kecambah yang disebabkan R. solani.

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Biological control of Fusarium wilt of tomato with hypovirulent binucleate Rhizoctonia in greenhouse conditions

Cited by 36 publications

References 37 publications

Bacillus methylotrophicus Strain NKG-1, Isolated from Changbai Mountain, China, Has Potential Applications as a Biofertilizer or Biocontrol Agent

Bacillus methylotrophicus Strain NKG-1, Isolated from Changbai Mountain, China, Has Potential Applications as a Biofertilizer or Biocontrol Agent

A Rapid Bioassay to Evaluate Efficacy of Hypovirulent Binucleate Rhizoctonia in Reducing Fusarium Crown and Root Rot of Tomato

Fusarium Nonpatogen sebagai Agens Hayati Penyakit Rebah Kecambah pada Tanaman Terung

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