Biological control of gastrointestinal nematodes in horses fed with grass in association with nematophagus fungi Duddingtonia flagrans and Pochonia chlamydosporia

Carmo, Tábata Alves Do; Mena, Mateus Oliveira; Cipriano, Isabela de Almeida; Favare, Giordani Mascoli de; Guelpa, G; Pinto, Sara da Costa; Amarante, Alessandro Francisco Talamini do; Araújo, Jackson Víctor de; Soutello, Ricardo Velludo Gomes de

doi:10.1016/j.biocontrol.2023.105219

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…Biological control is one of the options to complement helminth control actions. Helminthophagous fungi have been shown to be effective in eliminating the free-living stages of helminths in pastures, decontaminating the environment, and reducing consequently the reinfection of animals [ 11 , 12 , 13 ]. These fungi act during the free-living stages of helminths [ 14 ] and exert their predatory action through different mechanisms of action, capturing and destroying the parasites [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Efficiency of Experimental Formulation Containing Duddingtonia flagrans and Pochonia chlamydosporia against Moniezia expansa Eggs

Ribeiro,

de Almeida Moura,

e Silva

et al. 2023

Pathogens

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This study aimed to evaluate the effectiveness of the experimental formulation containing chlamydospores of Duddingtonia flagrans and Pochonia chlamydosporia fungi, against Moniezia expansa. Two experiments were carried out. The first experiment evaluated the in vitro efficacy using 1 g of the experimental formulation (V1) added to 100 M. expansa eggs and the control (V2) (without the fungal formulation). Intact eggs or eggs with alterations were counted in order to evaluate their effectiveness. The second experiment evaluated the action of the fungal formulation on M. expansa eggs after passing through the gastrointestinal tract of goats. Three groups were identified as B1, B2, and B3, which received 1.0, 1.5 g of experimental fungal formulation, and placebo, respectively. In experiment 1, all the eggs in V1 were subjected to the predatory action of fungi, while in V2, the eggs remained intact. In experiment 2, the reduction of eggs in groups B1 and B2 were 49% and 57% 24 h after ingestion, 60% and 63% 48 h after, and 48% and 58% 72 h after. The predatory capacity against M. expansa eggs shown in the tests demonstrated that experimental fungal formulation has the potential to be used on integrated helminth control programs.

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Section: Introductionmentioning

confidence: 99%

Efficiency of Experimental Formulation Containing Duddingtonia flagrans and Pochonia chlamydosporia against Moniezia expansa Eggs

Ribeiro,

de Almeida Moura,

e Silva

et al. 2023

Pathogens

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show abstract

Nematophagous fungus Pochonia chlamydosporia to control parasitic diseases in animals

Fonseca

Castro

Carvalho

et al. 2023

Appl Microbiol Biotechnol

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Biological control of the native endophytic fungus Pochonia chlamydosporia from the root nodule of Dolichos lablab on Fusarium wilt of banana TR4

Zhou,

Yang,

et al. 2024

Front. Microbiol.

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Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense, Tropical Race 4 (TR4) is a soil-borne disease, and it is devastating. At present, the biological control using antagonistic microorganisms to mitigate TR4 is one of the best strategies as a safe and green way. Yunnan has abundant and diverse microbial resources. Using the dual-culture method, the antagonistic endophytic fungi against TR4 were isolated and screened from the root nodule of Dolichos lablab. The effect of the highest antagonistic activity strain on the morphology of the TR4 mycelium was observed using the scanning electron microscope. According to morphological characteristics and sequence analysis, the strain was identified. The biocontrol effect and plant growth promotion were investigated by greenhouse pot experiment. Using the confocal laser scanning microscope and the real-time fluorescence quantitative PCR, the dynamics of TR4 infestation and the TR4 content in banana plant roots and corms would also be detected. In this study, 18 native endophytic fungi were isolated from a root nodule sample of Dolichos lablab in the mulch for banana fields in Yuxi, Yunnan Province, China. The YNF2217 strain showed a high antagonistic activity against TR4 in plate confrontation experiments, and the inhibition rate of YNF2217 is 77.63%. After TR4 culture with YNF2217 for 7 days in plate confrontation experiments, the morphology of the TR4 mycelium appeared deformed and swollen when observed under a scanning electron microscope. According to morphological characteristics and sequence analysis, the strain YNF2217 was identified as Pochonia chlamydosporia. In the greenhouse pot experiment, the biocontrol effect of YNF2217 fermentation solution on TR4 was 70.97% and 96.87% on banana plant leaves and corms, respectively. Furthermore, YNF2217 significantly promoted the growth of banana plants, such as plant height, leaf length, leaf width, leaf number, pseudostem girth, and both the aboveground and underground fresh weight. Observations of TR4 infestation dynamics in banana roots and corms, along with real-time fluorescence quantitative PCR, verified that YNF2217 inoculation could significantly reduce the TR4 content. Therefore, YNF2217 as P. chlamydosporia, which was found first time in China and reported here, is expected to be an important new fungal resource for the green control of Fusarium wilt of banana in the future.

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Biological control of gastrointestinal nematodes in horses fed with grass in association with nematophagus fungi Duddingtonia flagrans and Pochonia chlamydosporia

Cited by 3 publications

References 32 publications

Efficiency of Experimental Formulation Containing Duddingtonia flagrans and Pochonia chlamydosporia against Moniezia expansa Eggs

Efficiency of Experimental Formulation Containing Duddingtonia flagrans and Pochonia chlamydosporia against Moniezia expansa Eggs

Nematophagous fungus Pochonia chlamydosporia to control parasitic diseases in animals

Biological control of the native endophytic fungus Pochonia chlamydosporia from the root nodule of Dolichos lablab on Fusarium wilt of banana TR4

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