Biological control of Oryctes Rhinoceros (L.) using an indian isolate of Oryctes baculovirus

Mohan, K. S.; Pillai, G. B.

doi:10.1017/s1742758400017926

Cited by 5 publications

(1 citation statement)

References 19 publications

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“…This may not be surprising, considering how few baculoviruses are used for microbial control, but such studies can be fruitful in cases where the virus also plays a large role in the natural population dynamics of a pest insect and may persist through dissemination and transmission (27,(61)(62)(63)(64)(65). In the case of LdMNPV, field transmission experiments can help to predict the total impact of a biopesticide over the field season.…”

Section: Discussionmentioning

confidence: 99%

Deletion ofv-chiAfrom a Baculovirus Reduces Horizontal Transmission in the Field

D’Amico

Slavicek

Podgwaite

et al. 2013

Appl Environ Microbiol

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f Nucleopolyhedroviruses (NPVs) can initiate devastating disease outbreaks in populations of defoliating Lepidoptera, a fact that has been exploited for the purposes of biological control of some pest insects. A key part of the horizontal transmission process of NPVs is the degradation of the larval integument by virus-coded proteins called chitinases, such as V-CHIA produced by the v-chiA genes. We used recombinant and naturally occurring strains of the Lymantria dispar NPV (LdMNPV) to test horizontal transmission in the field, release of virus from dead larvae under laboratory conditions, and cell lysis and virus release in cell culture. In the field, strains of LdMNPV lacking functional v-chiA genes showed reduced horizontal transmission compared to wild-type or repaired strains. These findings were mirrored by a marked reduction in released virus in laboratory tests and cell culture when the same strains were used to infect larvae or cells. Thus, this study tests the pivotal role of liquefaction and the v-chiA gene in field transmission for the first time and uses complementary laboratory data to provide a likely explanation for our findings.

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